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Isolation and identification of unknown bacteria... can suggest that the unknown bacteria is from the Klebsiella spp. but there are other bacteria that are gram negative, oxidaze negative, lactose positive like Escherichia colli or Enterobacter aerogenes. That is why we need to do additional tests in order to confirm our findings. Urea broth test: Our Urea Agar Slants can be used to aid in the Recommended Procedure differentiation between members of the Enterobacteriaceae family based on their ability to hydrolyze the substrate urea . Urease is an important microbial enzyme concerned with the decomposition of organic compounds. Urease can act on the test substrate, urea, contained in the medium resulting in the release of ammonia. The release of this alkaline end product results... produce...
11 Pages(2750 words)Lab Report
Microbiological effects from baby bottle misuse.... Given the overwhelming proof for the distinctions in the health consequences, breast feeding must be appreciated as the biologic standard for child feeding. Different physician advice, office and health centers conduct must be aligned to ascertain that breastfeeding mother-infant dyad leads to the best opportunity for a long lasting experience in breast feeding. Reference Agostoni, C., et al. (2004).Preparation and handling of powdered infant formula: a commentary by the ESPGHAN Committee on Nutrition. Journal of Pediatric Gastroenterology and Nutrition, 39, 320-322 Drudy, D., Mullane, N., Quinn, T., Wall, P., & Fanning, S. (2006). Enterobacter sakazakii: An emerging pathogen in powdered infant formula. Clinical...
4 Pages(1000 words)Research Paper
Differentiation of Microorganisms...aerogenes, Salmonella enterica , Pseudomonas aeruginosa, Staphylococcus aureus , and Enterococcus faecalis. The three media were Glucose, Sucrose, and Lactose. All the microorganism exhibited growth in the three media except Salmonella enterica and Enterococcus faecalis which exhibited no growth in sucrose and lactose respectively. Additionally, the experiment tested the fermentation of Glucose, Sucrose and Lactose between 2 to 4 days and the results were recorded. In Glucose media Acid and gas were produced in Escherichia coli , Enterobacter aerogenes, and Salmonella enterica , no color change in Pseudomonas aeruginosa, while Staphylococcus aureus , and Enterococcus faecalis produced...
5 Pages(1250 words)Lab Report
Lab Report: Nutritional Growth of Bacteria...Lab Report: Nutritional Growth of Bacteria This study focused on detecting and observing the bacterial growth by supplying and providing it the nutrients needed to grow its colony. At the end of this study, the facts that deal with the role of various nutrients in the bacterial growth were presented for analysis and discussion. It comprises the speed of its growth. This also deals with the entire period of time which can hold and manipulate a number of not just a single bacteria but the growth of the whole family of the microorganisms we have. We considered the factors that affects this bacteria's such as the Escherichia Coli, Staphylococcus aureus, Enterobacter aerogenes, Staphylococcus epidermiditis, Salmonella enteriditis... )
Mannitol...
8 Pages(2000 words)Essay
Biochemical identification of unknown bacteria...that the organism can grow aerobically or anaerobically depending on the culture conditions.
Nutrient gelatin:
Gelatin was found to be liquefied after incubation indicating gelatinase activity
Physiological characteristics (fermentation and oxidative tests):
Standard results
CONTROLS
O/F
Durham
Citrate
Mixed acid
V-P
Oxidase
Catalase
NO3
NG
FTM
A/An
Dex
Man
Lac
Pseudomonas aeruginosa
+/-
-/-
-/-
-/-
+
-
-
+
+
-
+
A
Escherichia coli
+/+
+/+
+/+
+/+
-
+
-
-
+
+
-
F
Enterobacter aerogenes
+/+
+/+
+/+
+/+
+
-
+
-
+
+
-
F
Staphylococcus aureus
-/-
+/-
+/-
+/-
-
+
-
-
+
+/-
+
F
- Results obtained for unknown...
4 Pages(1000 words)Lab Report
Microbiology-microorganism identification...Results – Lab #6: The identification of bacteria Bergey’s Manual DIAGNOSTIC TABLE: Please that many characters can vary with strain type. Characters* = Most Strains
Litmus Milk: Acid (A); Clot (C)
All the unknowns used in this laboratory are presented.
Organism
Pigment Production
Cell Shape
Cell Arrangement
Gram Stain
Spore Production
Motility
Opt. Growth Temp.oC
Aerobic Growth
Anaerobic Growth
Catalase
Oxidase
Oxidative
Fermentative
Citrate utilization
Nitrate production
Indole Production
Glucose fermentation
Lactose fermentation
Sucrose fermentation
Methyl Red (MR)
Voges-Proskauer (VP)
Gelatin Hydrolysis
Litmus Milk
Bacillus subtilis
_
R
Seldom in chains
+
+
+
37
+
_
+
*_
+
+
_
+
_
_
_
_
+
*C
Enterobacter...
2 Pages(500 words)Assignment
The role of e.coli ESBL+...of naturally occurring TEM-like enzymes which contains a number of amino acid substitutions and deletions not noted in other TEM enzymes. These naturally occurring TEM-type ESBLs results from fluctuation in selective pressure from several β lactams within a given institutuin and not because of selection by a single agent.
Although,TEM type β lactamases are frequently found in E. coli and K. pneumoniae, they can also be found in increasing numbers in other Gram negative bacteria.They have been reported in Enterobacter aerogenes, Morganella morganii, Proteus rettgeri, Proteus mirabilis and Salmonella spp. Inhibitor resistant β lactamases are derivatives of classical TEM or SHV-type enzymes but are not...
18 Pages(4500 words)Literature review
Citrobacter freundii...Figure showing flowchart to determine the identity of the organism:
Unknown #20
Gram stain
Gram negative rod
Oxidase test (positive)
Positive negative
Citrobacter freundii Proteus vulgaris
Enterobacter aerogenes Proteus mirabilis
Escherichia coli Serratia marcescens
Klebsiella oxytoca
Indole test (positive)
(Positive) (negative)
Escherichia coli Citrobacter freundii
Klebsiella oxytoca Enterobacter aerogenes
Klebsiella pneumonia
Citrate test (negative)
(Positive) (negative)
Citrobacter freundii Klebsiella pneumonia
Enterobacter aerogenes
Motility (negative)
(Positive) (negative)
Citrobacter...
1 Pages(250 words)Lab Report
Ostemyelitis...of different stages of osteomyelitis. Also some predisposed factors were discovered such as malnutrition, debilitating diseases, immunological and genetic factors. Therefore, through a long historical period scientist describe pathology, diagnosis, treatment of the osteomyelitis. (Abulfotooh M. Eid,2003)
Osteomyelitis usually caused by bacteria, but may be provoked by fungi or other germs. Different bacteria can cause osteomyelitis depends on age of the patients. Newborns at age of under 4 months are infected by Staphylococcus aureus, Enterobacter and group A and B Streptococcus species. Children at age 4 months -4 years can be infected by Haemophilus influenza, S. aureus, Enterobacter and group A...
4 Pages(1000 words)Research Paper
MLT1 Task 5...to red (McDevitt, 2009).
Uses of Voges-Proskauer test
The Voges – Proskauer test is appropriate because of the detection capability of acetoin, which is produced by the bacteria that use it as metabolic intermediate by following the butane diol pathway (Buchanan & Cibbons, 1974). This test is used when differentiating Escherichia coli from other Enterobacter species like Enterobacter aerogenes because E. coli is positive in Methyl red yet negative Voges-Proskauer whiles the Enterobacter aerogenes is negative in Methyl red test and positive in Voges-Proskauer (McDevitt, 2009).
Organisms using alternative pathways to metabolise glucose
Availability of main...
1 Pages(250 words)Essay