PCR Determination of Optimum Parameters in the Analysis of Pten and Its Role in Aging and Senescence - Research Paper Example

Traditional PCR is used for amplifying DNA for sequencing, genotyping, and cloning. Traditional PCR as was used in this experiment has several shortcomings namely; poor precision, low sensitivity, the short dynamic range of fewer than 2 logs, low resolution, nonautomated, based on discriminatory size only, and results cannot e expressed as numbers, the staining agent ethidium bromide is not efficient at quantification and finally, post PCR processing is required. This paper looks into the analysis of PTEN using the traditional PCR method.

The process was not successful despite the necessary steps being undertaken. GADPH primer when used on a Hacat cell line showed annealing at 62 degrees. However, the use of PTEN with similar parameters which were later altered for optimization yielded no results. The methodology has been discussed and the gel results showing synthesized images have been included in this report. The discussion that covers the PCR process and its effectiveness has also been included. The fidelity of the PCR process has been discussed to extrapolate on the possible reasons for the failure of this analysis.

Concluding remarks at the end of the report summarize the experiment Phosphatase and Tensin Homolog gene commonly referred to as the PTEN gene provides the necessary instructions for the synthesis of a protein that is present in almost all body tissues (Sulis, 2003). The protein is significant in its function as a tumor suppressor and aids in the regulation of cell division by inhibiting cells from diving and growing too fast or uncontrollably. PTEN is a phosphatase that modifies other fats and proteins through the removal of phosphate groups comprising one phosphorus atom and three oxygen atoms. Further, the PTEN protein forms part of a chemical pathway that signals cells to cease dividing triggering them to self-destruct via apoptosis. The PTEN protein aids in controlling cell migration, adhesion, and angiogenesis.