Neuronal and Cellular Signalling: Preparation and partial characterisation of rat brain synaptosomes, myelin and mitochondria - Lab Report Example

Comments (1) Cite this document
For example, for sample h the absorbance was 0.068, and the interpolated amount was 1.104 µg.
Theoretically, the values in test tubes 3 and 4…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER94.1% of users find it useful
Neuronal and Cellular Signalling: Preparation and partial characterisation of rat brain synaptosomes, myelin and mitochondria
Read TextPreview

Extract of sample "Neuronal and Cellular Signalling: Preparation and partial characterisation of rat brain synaptosomes, myelin and mitochondria"

Download file to see previous pages In turn, oligomycin will inhibit the synthesis of ATP of mitochondria as well as glycogenolysis and glycolysis respectively. This resulted to the problems encountered during the rate of choline uptake by the synaptosomes. The composition of this mixture with the choline is identical to the Na+ ion medium, and there was the replacement of NaCl by the choline chloride respectively.
The addition of Normal 1 and normal to the test-tubes will generally lower the inhibition rate as compared to 5, 6(deoxyglucose ) and oligomycin. With the lowered inhibition rate, the will be an increase in choline uptake by the synaptosomes. ATP synthesis will also be inhibited as well leading to the increase in choline uptake.
Similarly, when 6(2-deosyglucose and olygomycin will also induce chemical anoxia in the synaptosomes as well as the mitochondria, however, there will be a slight increase synthesis of ATP inhibition as well as glycogenolysis and glycolysis that will eventually further increase the rate of choline uptake that will slightly increase.
The protein content was highest in the pure mitochondrial fraction and lowest in the p2 fraction. The order of the distribution of proteins from the highest to the lowest was pure mitochondria, myelin, partially pure mitochondria, synaptosomes, homogenate and P2 fraction. This distribution implies that there is little protein in the synaptosomes and that the higher quantity of protein in the pure mitochondrial fraction can be attributed to the presence of various respiratory enzymes. My recovery process was efficient because the sum of the percentages of the different fractions as proportions of P2 pellet protein gave 99.9%.
There exist mechanisms of choline uptake namely a saturable high-affinity and a high capacity low-affinity uptakes (Blusztajn and Wurtmman 617). Hemicholinium 3 inhibits the uptake of high-affinity choline uptake, but poor in ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“Neuronal and Cellular Signalling: Preparation and partial Lab Report”, n.d.)
Retrieved from
(Neuronal and Cellular Signalling: Preparation and Partial Lab Report)
“Neuronal and Cellular Signalling: Preparation and Partial Lab Report”, n.d.
  • Cited: 0 times
Comments (1)
Click to create a comment or rate a document
ameliaboyle added comment 9 months ago
Student rated this paper as
This lab report was always my weak point. I could never finish it on time. Still, after I found this particular paper, I understood how it should be completed. So, I performed my research afterward and completed the lab report in several hours, instead of weeks as it was previously.

CHECK THESE SAMPLES OF Neuronal and Cellular Signalling: Preparation and partial characterisation of rat brain synaptosomes, myelin and mitochondria

Partial-birth abortion

...not recognize that she was pregnant; they had financial difficulty in arranging for an earlier term abortion; they were afraid to tell others of the pregnancy; they needed the time to make the decision; they felt that they should not follow through with it; they did not know they could get an abortion; and they found out later in the pregnancy that the fetus was abnormal. In addition, although extremely rare, there are abortions that take place after 20 weeks, which are most commonly referred to as partial birth abortions. There are three common abortion options, which are medication abortion, aspiration abortion and partial birth abortion. With medication abortions, the mother is prescribed pills that...
8 Pages(2000 words)Essay

Rat trap boat evaluation

...? Rat Trap Boat By Your Your School May 28, Rat Trap Boat Most of the time when a rat trap is pictured it isusually in association with cheese, or peanut butter, being set out to catch a pesky home invader. All though some may also envision the all too famous Tom and Jerry episode where Tom’s tail gets caught in the trap he has “cleverly” laid out for Jerry. In today’s ever changing, always inventive, society, however, the rat trap has come to serve a different, unique purpose than perhaps was ever intended. Many students of engineering and design have found that the powerful force and motion created from the trap can be harnessed and used to power mini vehicles of all...
5 Pages(1250 words)Essay

The Origin of Mitochondria

...?Running head: THE ORIGINS OF MITOCHONDRIA The Origins of Mitochondria The origins of mitochondria remain an important object of professional interest. Dozens of researchers explored the origins of mitochondria and their implications for future research. This paper provides a brief summary of the article Microsporidia, Amitochondrial Protists, Possess a 70-kDa Heat Shock Protein of Mitochondrial Evolutionary Origin. The purpose, methods, and results of the study are discussed. The information from the article is compared to what other articles say. Recommendations for future research are provided. Keywords: mitochondria, origins, microsporidia, evolution....
3 Pages(750 words)Book Report/Review

Aerobic Cellular Respiration in Isolated Mitochondria of Lima Bean Seeds

...? Investigating the Aerobic Cellular Respiration in Isolated Mitochondria of Lima bean (Phaseolus lunatus) seeds during the Conversion of Succinate to Fumarate in Krebs cycle Introduction: A basic concept in cellular respiration is the transfer of chemical energy (electrons) from an electron donor (i.e. carbohydrate, proteins and fat from food molecules) to an electron acceptor. Most of the organisms use oxygen as the final electron acceptor although some organisms (eg. Yeast) use other alternatives. During aerobic respiration, oxygen in the air is used as the final electron receptor which subsequently gets reduced to water. Energy is generated during this process in the form of a high energy molecule, adenosine triphosphate (ATP... , the...
6 Pages(1500 words)Lab Report


...? SHOULD THE U.S. BUILD MORE NUCLEAR POWER PLANTS FOR AN ENERGY SOURCE? The US has over the years been faced with variouschallenges rising from the high demand of energy, unreliable supply of energy, air pollution concerns, climate change and high prices of fossil fuel. A concern over US’s overdependence on the Middle Eastern oil and need for alternative energy have pushed the US government into considering nuclear power. The government has decided to build new nuclear power plants and revive old ones. However, this option by the government has met its fair share of both positive and negative criticism. Those against it argue that there are high risks of accidents and production, maintenance, and nuclear plants operation costs... SHOULD THE...
5 Pages(1250 words)Coursework

Brain neuronal Cb2 cannabinoid receptors in drug abuse and depression

...? Brain Neuronal Cb2 Cannabinoid Receptors in Drug Abuse and Depression: From Mice to Human s Brain Neuronal Cb2 Cannabinoid Receptors in Drug Abuse and Depression: From Mice to Human Subjects Background of the Study The stressful events might cause addiction to substances and major depression within the context of mental health problems. Many humans go through high relapse cases and reoccurrence of the cases even after they undergo treatment. Laboratory detecting procedure does not show the presence of cannabinoid CB2 receptors (CB2-Rs) in a healthy brain. The demonstration in rats indicates the presence of CB2-R in microglia and other...
5 Pages(1250 words)Assignment

The Signalling theory

...? THE SIGNALLING THEORY by + of The Signalling Theory Signalling theory posits the thought of sharing information. In economics, this is where one group plausibly conveys information to another about themselves. The theory originated from the concept of asymmetric information. In this case, business transactions were conducted in an unbalanced manner. The signalling theory thus provides equilibrium in relation to access to transactional information, allowing the parties involved to transact effectively. Similarly, human interactions rely on signals most of the time. The signals enable people to identify some hidden qualities of the other person. The theory focuses on providing comprehension of the varied signals as well as noting... , which...
7 Pages(1750 words)Essay

Rat Temple in India

...that female maternity rats are taken in private to give birth and then returned after the process is complete. A devotee has to prepare Prasad in big cauldrons of iron, whenever their wishes are fulfilled and this is shared among the other devotees. Contrary to the fast-reproducing nature of rats, population of rats at the Temple has remained constant over time. Notably as well, they all have a standard appearance and weight, and this adds on to the mystic feature of the Kabbas or rats at the Karni Mata Temple. Overall, it is evident that religion has greatly influenced architectural design and style in India as illustrated by the Karni Mata Temple. The...
4 Pages(1000 words)Essay

The Rat vs. the Rabbit

...Response to The Tale of One Bad Rat “The tale of One Bad Rat” is the story of a victim of child abuse, who relies upon the imagery of Beatrix Potter’s animal stories in order to preserve her sanity when faced with a series of traumatic incidents. The author of “The Tale of Peter Rabbit” –Beatrix Potter – features prominently in the book, with the protagonist also sharing the same first name as the author. Potter’s story about Peter rabbit is fraught with adventure and the animal protagonist goes through a series of misadventures and unsafe situations before finding safety at the end of the book. Helen’s story in “The Tale of One Bad Rat” parallels the crises that erupt in the rabbit...
3 Pages(750 words)Essay

The Desert Rat-Kangaroo

...Environmental Studies Environmental Studies The Desert rat-kangaroo (Caloprymnus campestris) Discovered in the 1840s and previously common in the desert areas of Central Australia, the desert rat-kangaroo was officially listed as extinct under the 1999 Act of Environmental Protection and Biodiversity (Fisher & Blomberg, 2010). Apart from females with young offspring that lived in nests, it was typically solitary and its native habitat was arid land with sparse cover although it was also found in Australia’s loamy flats and clay pans. However, its extinction is still marred in ambiguity mainly because there were reports of its rediscovery, albeit short-lived, after its 90-year disappearance since the first...
4 Pages(1000 words)Essay
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Lab Report on topic Neuronal and Cellular Signalling: Preparation and partial characterisation of rat brain synaptosomes, myelin and mitochondria for FREE!

Contact Us