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Lab Report Practical Fluorescence Microscopy Practical...?Laboratory report on Practical Fluorescence Microscopy Practical a & b Use and imaging of cultured animal cells Submitted By Bearing student no Your student number On Date For the Course (CELB40100) Summary: A laboratory work was done on fluorescence microscopy with an aim to get practical knowledge on the use and imaging of cultured animal cells. For this study, pre-cultured HeLa cells were counted and then plated on coverslips placed in culture dishes. The cells, attached to the coverslips, were washed with phosphate buffered saline (PBS) and fixed using chilled methanol or paraformaldehyde (PFA), depending upon the protein to be stained. The PFA fixed cells were permeabilised using...
7 Pages(1750 words)Essay
PV92 practical...? Practical Techniques in Genomics and Proteomics Respond to Question It is importance to Chelate metal ions from solution in theprocess of boiling step at 100 degrees Celsius because chelating agents are chemicals that often form complex, and soluble, molecules with specific metal ions (Ashmead & DeWayne, 1993). They are essential since they help inactivate the ions thus making them not to be able to react normally with other ions and elements in order to produce scale or precipitates. Given that most enzymes such as the DNases need metal ions for purposes of using it as a cofactor, it implies that lack of InstaGene matrix would lead to a situation where the enzymes are not inactivated. This would, therefore, mean that...
7 Pages(1750 words)Assignment
Practical...? Practical Report on Children and Young People’s Self Identity Children and young people exhibit differences in the way they behave and how they relate with other people, which constitute their personality and individual character traits. This largely involves how children develop a sense of identity in the way they see themselves and how other people view them. This sense of identity develops and changes through a period of time along their childhood. Every person has a unique gradual development of a sense of autonomous and individuality which is relatively different from other people. This concerns the child’s social behavior and his own view about how people relate with him or her depending on the expectations of...
7 Pages(1750 words)Essay
Practical report...hybrids with complementary single strand template DNA strands resulting into short double-strand regions that extend by a DNA polymerase in 5’ to 3’ direction. This results in an exponential buildup of the definite target section, approximately 2n where n is the amount of cycles of magnification performed. Figure 2 below shows a pictorial expression of PCR. Fig 2 3.0 Aim and Hypothesis The aim of this project was to investigate purification of genomic DNA from buccal cells by use of Agarose Gel Electrophoresis. Practical 1: Purification of Genomic DNA from Buccal Cells 4.1 Methods and Materials 4.1.1 Materials 10 ml of 0.9% saline, sterile 50 ml Falcon Tube, centrifuge, stop watch, pipette, Chelex beads,...
8 Pages(2000 words)Essay
Practical...? Practical: Examining 6 to 12 Year Old Children’s Understanding of Why Some Objects Float and Others Sink This study tried to explore how children of different ages explain why some objects float while others sink. Data was collected from two female participants between the ages of 6 - 12 years who studied in the same school via a video-recorded session with a researcher. Each child was shown a number of items and asked to predict whether it would float or sink and to provide explanations. They were also challenged on inconsistencies. It was found that children did indeed try to develop a consistent theory for why some objects float and others sink, and that maturity was associated with more complex explanations. The involvement... ...
10 Pages(2500 words)Essay
DNA Practical...Aim To isolate the DNA from the tissues of kiwi fruit and to study how to visualize DNA using gel electrophoresis. Introduction DNA is the genetic material responsible for the development of all forms of life. It is found in the nucleus, mitochondria and chloroplast (in plant cells). In nucleus, DNA is associated with the histone proteins and is linear in shape whereas in organelles like mitochondria and chloroplasts, DNA is circular and is not associated with histone protein. The DNA from mitochondria and chloroplast is responsible for cytoplasmic inheritance or maternal inheritance.
DNA is a double stranded nucleic acid and both the strands run antiparallel. It is made up of pentose sugar (de-oxy ribose), phosphate group... To isolate the...
4 Pages(1000 words)Lab Report
Pharmacology Practical...PANCREATIC ASSAY INHIBITION ASSAY ID Pharmacology Practical 09 December Pancreatic Lipase Inhibition Assay
Results
Table 1: Absorbance values and final Orlistat concentration after incubation
Tube no.
Final conc. Orlistat
(µg/mL)
Absorbance at 410 nm (A1)
Absorbance at 500 nm (A2)
Absorbance
A1 – A2 = A3
1
100
0
0
0
2
80
0.098
0.010
0.088
3
60
0.078
0.008
0.007
4
40
0.121
0.061
0.006
5
20
0.104
0.031
0.073
6
10
0.123
0.019
0.104
7
5
0.190
0.110
0.08
8
1
0.123
0.040
0.083
9
0.5
0.156
0.060
0.096
10
0
0.361
0.188
0.173
Figure 1: Plot of absorbance values and final Orlistat concentration after incubation
Estimating IC50 value
One of the data points in the...
2 Pages(500 words)Essay
Practical 1... Annealing This process was performed on test pieces of copper at the temperature of 500ºC. The results obtained from the experiment give an approximated behavioral pattern of the various cold worked samples of copper. During the process, it was observed that the specimen have increasing roughened surfaces as the degree of rolling is increased. This is due to dislocation pile up at the grain boundaries. The hardness of the sample during cold rolling increases with increase in reduction in the cross-sectional area or degree of deformation. This is attributed to strain hardening. The maximum value of hardness was obtained at 70% reduction in area.
(a) What property must be exceeded for rolling to successfully change a materials... Annealing...
1 Pages(250 words)Assignment
Practical Life...Montessori: Practical life Insert Insert Montessori became one of the most influential shapers of the school systems. She came up with innovative classroom practices that have since shaped the education of young children. She graduated as a medical doctor from Rome medical school. Her passion in education drove her to help young children with special needs. Through her practices, she concluded that children learn best by doing. In addition, happy self-motivations assisted the children develop better. The discovery led her to write articles and journey across the world establishing schools to serve children. For example, coming up with a Montessori classroom and environment oriented to...
4 Pages(1000 words)Research Paper
Practical...Database Server Management Oracle SQL Table of Content Database Server Management Introduction......................................................................................................................................3
Entity Model....................................................................................................................................5
Tables and the Fields with Test Data...............................................................................................7
Stages in the Software Development Life Cycle.............................................................................8
Task 2... ...
10 Pages(2500 words)Assignment
