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Blood Grouping,Blood staining and haematocrit ( laboratory report) - Assignment Example

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BLOOD GROUPING, BLOOD STAINING, AND HEMATOCRIT Laboratory Report 06 March 2013 Introduction The ABO blood grouping entails the categorisation of human blood depending on heritable traits of red blood cells as established by the existence or absence of antigens (Rogers, 2011)…
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Blood Grouping,Blood staining and haematocrit ( laboratory report)
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Download file to see previous pages It is essential for healthcare practitioners to determine one’s blood group to know which blood type is fit for the patient in case a blood transfusion or transplant is performed. This is because not all blood types are compatible. If someone is transfused with blood that is different from their blood group, the individual’s antibodies will mount an immune response against the received blood and destroy the red blood cells in the donor’s blood (Rogers, 2011). Blood grouping can also be used to determine paternity because an offspring’s blood group is determined by the individual genotypes in both parents’ blood. Aim This experiment aimed at determining the blood group, Rhesus factor, and hematocrit value of the subject’s blood sample. Hypothesis The experimental hypothesis was that the subject’s hematocrit value was within the normal range. Method Blood Grouping The subject was invited to sit, and it was ensured that they were comfortable. The rationale of the tests was explained to the subject, after which their informed consent was obtained. The subject was instructed to wash their hands using warm water to clean them and increase circulation. The hands were dried using a paper towel. The puncturing device was prepared with a new sterilized automatic lancet. A clean, white tile was marked A, B, AB, and RH while leaving space for blood drops and was placed in proximity with the subject. The fingers were wiped using Mediswab and allowed to dry. Blood was drawn from the lateral pads of the fingers by a quick stab of the automatic lancet and allowed to fall onto each of the four labelled spaces on the white tile. One drop of antibody A (Anti-A) serum was added to the blood drop labelled A, one drop of Anti-B (antibody B) was added to the blood drop labelled B, one drop of antibody AB (Anti-AB) serum was added to the blood drop labelled AB, and one drop of Rh serum was added to the drop labelled Rh. The blood drops were mixed with the sera and the observed for agglutination. The results were read after 2 minutes and recorded. Hematocrit A plain capillary tube was filled with the mixed anti-coagulated blood provided, or a heparinised tube with blood obtained by finger prick. One end of the tube was dipped into the blood. The capillary tube was tilted horizontally to allow the blood to flow up the tube, which was filled up to 1 inch from the end. The unfilled end of the tube was sealed with Cristaseal seal and placed in a numbered slot in the microhematocrit centrifuge. It was ensured that the sealed end was pointing outwards, and that all tubes were touching the outer rim. After centrifugation for 5 minutes, the tube was placed in the microhematocrit reader with the base line of the reader intersecting the base of the red cells. The tube holder was held until the top line intersected with the top of the plasma. The knob was then adjusted so that the white line intersected the top of the red cells. The %PCV was read on the scale and recorded. The capillary tube was disposed in the sharps box. Blood Staining A thin blood smear was prepared using a drop of blood on a glass slide and allowed to dry. 100% methanol was added to the slide and left to dry. The slide was then covered with Wright stain and was left on for 4 minutes. Running water was used to wash the slide gently. The underside of the slide was carefully wiped after which the slide was observed for ...Download file to see next pagesRead More
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