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Historical and Clinical Importance of the Sevier Munger Stain - Research Paper Example

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Running Head: Health Sciences and Medicine Historical and Clinical Importance of the Sevier-Munger Stain A Descriptive Paper Name Name of Professor Introduction Recently, several techniques for the silver staining of nervous tissues’ paraffin parts have been identified…
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Historical and Clinical Importance of the Sevier Munger Stain
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Download file to see previous pages The importance of carcinoids in rats has achieved significant consideration with the discovery that contact with herbicides lead to heightened pace of these tumors (Bruce-Gregorios, 2006). Contact with methyleugenol, which is commonly obtained in beverages and foods, is linked to heightened tempo of gastric carcinoids in rats as well. In numerous instances, the carcinoids could be minor to enlarged serum gastrin, enlarged gastric PH, and gastric-induced tropic impacts on ECL (Bruce-Gregorios, 2006). Sevier-Munger’s argyrophil silver stain was analyzed in rat thyroid parafollicular cells following Bouin fixation and formalin. The most simply reproducible and greatest staining reaction was acquired in Bouin-fixed and Sevier-Munger-stained tissues (Watts & Koller, 2004). Developments in methods of silver staining have frequently been gained from the chemistry field. The ammoniacal silver solution had been utilized for mirror making, rooted in the features of the intricate ‘silver diamine’ in the mixture that is predisposed to crystallize, after being condensed, at the border between the mixture and the surface of the glass (Hirth et al., 1988). This is referred to as the ‘silver mirror reaction’ and this was originally used in tissue staining by Fajersztajn. Soon after, Max Bielschowsky developed this technique by breeding formalin-fixed solidified areas in the silver nitrate before the ammoniacal silver solution (Hirth et al., 1988). Even though the precise process is yet to be explained, it is possible that this earlier administration of silver nitrate offers functional cores for later silver sedimentation in the ammoniacal silver mixture. In any case, it is logical to assume that the stepwise impregnation enhanced the staining perhaps by boosting the mass and level of silver precipitates (Hirth et al., 1988). Original adjustments of Bielschowsky technique were summed up by Beech and Davenport. The existence of so many adjustments, nevertheless, shows that this technique is very unstable and remains open to enhancements. An adjustment for paraffin-implanted pieces of typical neural tissues from different kinds of animal was originally suggested by Sevier and Munger (Uchihara, 2007). In the course of the pre-immunohistochemical period, silver stains were a crucial portion of the staining repository for classifying specific tissue structures and cell forms in tissue areas. Several of them were helpful in explaining endocrine cells, specifically in the gastrointestinal tract (Grimelius, 2004). Silver stains, until the latter part of the 1950s, were attended by several technical setbacks leading to unrestrained staining variables. A new set of silver stains were created for endocrine cell forms in the 1960s, and these stains produced outcomes that were capable of reproduction (Grimelius, 2004). The Sevier-Munger method shows thyroid’s C-cells and ECL, the Grimelius stain is a wide-ranging endocrine cell indicator, and the Masson stain shows the EC cells. Specifically in the pre-immunohistochemical period, these techniques of staining were frequently applied for histopathological examination (Grimelius, 2004). According to Uchihara (2007), the silver stains were created experimentally, and with a small number of omissions the chemical conditions is unidentified. Staining procedures are added. Sevier-Munger Method for Neural Tissue Staining This method is generally applied to show neurofibrillary tangles and neuritic plaques in the brain of ...Download file to see next pagesRead More
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