Toxicity of Compound in the Zebrafish Bioassay - Lab Report Example

The paper "Toxicity of Compound in the Zebrafish Bioassay" is a good example of a lab report on chemistry. For Dr. Pack, our UPenn gastroenterologist collaborator, to test the toxicity of compound 2 in the zebrafish bioassay, we provided Dr. Porter with the compound.

At the end of the chemical reaction session, a certain research grouped challenged that they were in a position to isoflavone using AlCl3in dioxane. In this case, the group did not state any conditions for this reaction to take place. As such, I decided to optimize the reaction conditions starting with the model compound Chromanone. In this case, I mixed chromanone and AlCl3 in dioxane and then refluxed the content for 3 hours. Unfortunately, I did not obtain positive results and hence I was prompted to repeat the procedure. In this attempt, I decreased dioxane’s volume from 50 ml to 20 ml, used 10% of AlCl3, and I prolonged the reaction time from 3 hours to 12 hours. During the reaction, I monitored the proceeding using TLC. However, I did not obtain the intended products after several tests. In the third attempt, I changed most of the conditions and measured used in the first two trials. In this case, I used 0.500 gm of chromanone in 20 ml dioxane and 0.40 mg of AlCl3 which represents 10% of the starting material. I also reduced the frequency of monitoring to 2 hours. After the first two hours, the starting materials had completely disappeared. This indicated that I had optimized the TLC condition, by using different ratios of ethyl acetate and hexane. Then, I ran prep-TLC and I realized 4 different compounds. I then separated these 4 new products and conducted tests by NMR. However, the 4 compounds did not match the expected NMR spectrum of the desired product.
My failure in these experiments prompted me to carry out research in journals and other materials. In this case, I came across some work discussing ring opining reaction on isoflavanones. The source has indicated all the conditions and the details about the experiment. However, the source suggests that I should use LiAlH4 which is a hazardous chemical. We had training with Dr. West on pyrophoric materials and now we are fully prepared to do the reaction. Read More