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Mass Spectrometry - PowerPoint Presentation Example

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Mass spectrometry is an analytical method of determining the molecular masses of compounds by producing spectra, which are used to determine the isotropic or elemental signature of a sample. The spectrum also is used to find the masses of particles and thereafter, the masses of…
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Mass Spectrometry
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Download file to see previous pages The ions are then separated according to charge to mass ratio. They thus deflect such that those with the same mass to charge ratio experience the same deflection. The results are displayed spectra. The molecules or atoms in the sample are identified by comparing the found masses with the known masses.
Mass spectrometry technology finds a wide range of applications such as in identifying unknown compounds, determining isotropic composition of element in a molecule and elucidating the structure of a compound.
The ion analyser: - fragment ions as well as molecular ions are accelerated by manipulation of the charged particles. The mass analysis uses negative and positive voltages to control the path of the ions. Uncharged molecules and fragments are pumped away. The paths the ions assume are determined by their charge to mass ratio.
Ionisation occurs at the ionizer stage. For soft ionisation, the bombardment which is carried out, impact very little residual energy onto the sample molecule resulting into very little fragmentation. Hard ionisation processes result into high residual energy into the sample material thereby leading t large fragmentation.
The diagram above show the mass spectrum of pentan-3-one. Here the commonest fragment oin(tallest peak) is at m/x = 5 in is important to note that this isn’t produced the same ion. The m/z =5 was produced by [CH3CH2CH2CH2]+ therefore, there is possibility of getting a particular fragment from pentane. The peak with the m/z =29 is produced my the ethyl ...Download file to see next pagesRead More
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