CHECK THESE SAMPLES OF Evaluation of Extraction Methods for Trypsin Enzymes From Corn Using a Colourimetric Protein Assay
This paper ''Enzyme-linked Immunosorbent assay'' tells that there are several environmental problems today which threaten the normal processes in our entire world.... One of these methods of detection includes the use of Enzyme-Linked Immunosorbent assay or ELISA
... nzyme-linked immunosorbent assay (ELISA) is a biochemical technique that allows rapid screening and quantification of an antigen in a sample.... CBs have a range of toxicity and vary in consistency from thin, light-coloured liquids to yellow or black waxy solids....
11 Pages
(2750 words)
Term Paper
Colourimetry is a hands-on application of the Beer-Lambert law to establish the proportion of light that is transmitted by a solution containing a coloured solute, and using that information to ascertain the concentration of the solute.... This experiment aimed at using colourimetry as a chemical technique to make a calibration curve of absorbance against concentration for known paracetamol concentrations and using it to obtain the concentration of paracetamol in the sample with the unknown concentration....
4 Pages
(1000 words)
Essay
In a colourimetric chemical test the intensity of the color from the reaction must be proportional to the concentration of the substance being tested.... ? In the most basic colourimetric method the reacted test sample is visually compared to a known color standard.... The chief advantage of colourimetric methods is that they provide a simple means for determining minute quantities of substances.... The principle behind any colourimetric technique for paracetamol analysis is that paracetamol undergoes oxidation-reduction reaction with an aqueous solution of iron(III) ion....
4 Pages
(1000 words)
Essay
Each reaction had the following component Ingredient Volume assay buffer 600µL ?... 3mM assay buffer 600?... ?Enzymes are favorably involved in processes such as breaking down of fat molecules, starch and large protein in food during digestion into smaller molecules, the nucleotides joining into DNA strands and phosphate group addition to ADP to make ATP (Schnell, 2002).... ONPG stock solution was 68mM, thus the required amount was calculated for each reaction using the equation c1v1=c2v2....
7 Pages
(1750 words)
Lab Report
The aim of protein assay is to understand how to draw a curve by using prepared solutions to establish the concentrations of unknown solutions of albumin using spectrophotometer.... protein assay is critical in the analysis of agricultural, industrial and biotechnological products.... This essay focuses on the determination of proteins using Biuret and Lowry assay techniques.... Biuret assay, which is the least sensitive assay is among the coulometric methods (Quereshi et al....
5 Pages
(1250 words)
Essay
Quantification of the assay is then made by measuring the amount of labeled second antibody bound to the matrix by the use of a colourimetric substrate (Please see the picture).... HRP-labeled antihuman IgG can be used to bind to target antigen in an assay system to localize even trace amounts of biomolecules.... When used for an assay this will give rise to a reaction like Different types of linked assays and their properties:There are mainly two types of ELISA assays....
3 Pages
(750 words)
Essay
Enzymes are biocatalysts, protein in nature that catalyze and regulate metabolic reactions.... The composition of enzyme is primarily a complex protein molecule and function by catalyzing specific biochemical changes within the bodies of animals and plants such as digestion of food in animals and photosynthesis in plants.... Moreover enzymes act as catalysts because they exhibit three dimensional protein structures.... enzymes acts on substrates and As stated by Onteh et al (2005), most biochemical reactions would be very slow without enzymes since enzymes increase the rate of biochemical reactions by thousands times....
8 Pages
(2000 words)
Lab Report
Proteinaceous waste and the soft fibrin found in inflammatory exudates can be effectively terminated by using proteolytic enzymes as anti-inflammatory agents.... Increased serum enzyme activity is mostly related to enzymes released from injured cells.... The time course of the increase in activity of the enzyme in serum is analogous to the time course of decrease in enzyme activity from a damaged organ (Jennings et al.... The paper describes the types, categories and functions of enzymes have promoted many findings in medical and life science....
11 Pages
(2750 words)
Literature review