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Identification of Microorganism - Lab Report Example

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The lab report demonstrates the experiments performed to identify the microorganism encompasses investigation of infection and microbial control. Also, explain why plasmids can provide their hosts with useful packages of DNA and what types of plasmids there are…
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Download file to see previous pages The staining procedure as followed by the differentiation step where the pathogenesis of the microbes was tested. In order to perform this, differential and selective media were used. The selective and differential media used in this experiment were Blood Agar (BA), MacConkey Agar (MAC) and Mannitol Salt Agar (MSA).
The Blood Agar (BA) identifies those groups of bacteria capable of secreting hemolytic enzymes lysing the red blood cells in Blood Agar. Blood Agar contains 5% suspension of red blood cells; therefore, these enzymes create a colorless zone around the colonies (Korolik and Beacham, 2008). Furthermore, Blood Agar is an enrichment medium that classifies organisms such as Streptococci (Korolik and Beacham, 2008). The vaginal swab 1, 2 samples and fecal sample were found to be non-hemolytic, therefore no obvious marks of hemolysis around the colonies were observed on the plates. The results indicate that the samples are devoid of Streptococci.
Another selective differential media is MacConkey Agar that comprises lactose, bile salts, crystal violet and neutral red (pH indicator). Crystal violet inhibits the growth of most gram-positive bacteria colony (Korolik and Beacham, 2008). Thus it was anticipated that the vaginal swab (2) specimen would contain no growth (non-lactose fermenting) on this media. This provides the evidence that supports gram-positive reaction which was recorded previously for the vaginal swap (2) in Report Form 1. Conversely, the vaginal swab (1) and fecal specimens produced results coherent with a lactose-fermenting organism. MAC is used for the selection and differentiation of the enteric gram-negative rods associated with the intestinal tract such as Escherichia coli (Tortora et al, 2007). Since the results of the vaginal swab (1) and fecal specimens were negative bacilli, it can be seen from their lactose fermentation that the unknown microorganisms are those belonging to the enteric group Escherichia coli or Salmonella. ...Download file to see next pagesRead More
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