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The coding gene downstream of the cloned promoter encodes luciferase reporter gene (Luc) - Assignment Example

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The direction in which the gene transcription takes places has an important role to play. The late genes of SV40 are expressed practically on the onset of viral DNA…
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The coding gene downstream of the cloned promoter encodes luciferase reporter gene (Luc)

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"The coding gene downstream of the cloned promoter encodes luciferase reporter gene (Luc)"

Download file to see previous pages When this construct is introduced into cells, luciferase will only be expressed if your gene of interest promoter has been activated. Hence the direction of the SV40 wild type viral promoter construct can be expressed in the following way :
Basically, when it comes to promoter construct , it varies and showcase high or low luciferase activity many a times .Generally ,the resulting high luciferase activity is due to the protein content in the specific promoter and vice ...Download file to see next pagesRead More
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