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Microtubules - Lab Report Example

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This insinuates that Clip accumulation is dependent on EB1 presence, especially with the acidic tail in question. The binding action…
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Extract of sample "Microtubules"

Download file to see previous pages This interaction, revealed by the structure, is branded the EB1-like motif and there is possibility that the binding interaction of CLIP family depends on this. Intuitively, considering that CLIP experiences binding at the acidic tail of tubulin, it is also possible that the acidic tail of EB1 also provides the same condition allowing for binding. 12
One of the important parts of the cell micro skeleton is the microtubules. The dynamics of microtubules is a very significant feature of cell control and cell morphogenesis. According to research carried out recently, microtubules are attributed with the role of maintaining intrinsic cell length control. Meanwhile, even after the development of a model that describes the MT dynamics that are associated with the control of cell length, elucidation of the spatial variability of MT nucleation is yet to occur.
This presentation is a case of an investigation on the dynamics that are associated with nucleation of microtubules. This presentation particularly refers to a Hela cell that has no visible chromosome which was videoed after aligning itself along a track of micro patterned fibronectin. This presentation also wanted to find out the quantitative distribution of catastrophes and velocities inside the cell’s cortical and non-cortical regions. It also sought to investigate the spatial distribution of the microtubule nucleation that had resulted.
According to the results obtained, concentration of microtubule nucleation could not be considered random as it was concentrated in particular regions of the cell. Also, the results showed that the cortical actin was instrumental in triggering the nucleation rate. The rate slowed down after the cortical actin layer was removed. After further examination of the microtubule lifetimes among the case and the control, it was found out that the slowing of nucleation rate could be attributed to the slowing down of the microtubule catastrophe rate, in the ...Download file to see next pagesRead More
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Microtubule Composition and Function in Eukaryotic Systems

...of which are microfilaments, then followed by intermediate filaments, and the largest kind of fiber are the microtubules. Of these three, the microtubules have the most involvement in the cellular processes within the cell, which can be attributed to the composition as well as the mode by which the network expands or retracts within the eukaryotic cell. Microtubules are made up of ?- and ?-tubulin dimers arranged in a lattice to create a series of protofilaments (Figure 1). 13 of these filaments are laid side-by-side to form 25nm tubes. Due to the head-tail arrangement ?- and ?-tubulin dimers, the whole microtubule network is considered to be polarized, with some of the...
4 Pages(1000 words)Essay

Molecular biology of the neuronal cytoskeleton

... of neural and other eukaryotic cells (Lasek, Oblinger and Drake, n.p). The structural elements of the neuronal cytoskeleton are visible via an electron microscope. This paper will analyze each component of the cytoskeleton and its morphological features. Microtubules are comprised of tubuline, a heterodimeric protein consisting of two 50kD subunits. These are known as alpha and beta, and exist in numerous isometric forms. Beta subunits can further be divided into four types which are Beta 1-4. There have been scientific speculations that the structure difference may have an influence on the composition and relative abundance of each form of beta tubulin (Georgiev, Papaioanou and Glazebrook, n.p). In addition, the properties of assembly...
3 Pages(750 words)Article

Immuno-labelling, fluorescence microscopy and live time-lapse imaging

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8 Pages(2000 words)Lab Report

Immunolocalization of the microtubule cytoskeleton

... wide microtubules that we will visualize with tubulin antibodies. The formation and maintenance of microtubules is cold sensitive. At 4C, microtubules are destabilized and they depolymerize. At 37C, human body temperature, they remain polymerized. Photograph 1 shows cells incubated at 4 0C, while photograph 2 shows cells incubated at 37 0C. At this stage of the procedure, microtubules cannot be detected in either of the photographs. Photograph 1 Photograph 2 3 separate plates are used to create the NRK cells culture. 2 of these plates are left to incubate at 37C, while the remaining one is left to incubate at 4C. Therefore 3 coverslips are extracted, 2 coverslips from the 37C plate and 1 coverslip from the 4C plate. These coverslips...
5 Pages(1250 words)Essay


... and depolymerisation, which are controlled by nucleotide GTP. Following synthesis and during activity, there are molecular rearrangements within these microtubules are stabilized with accessory proteins, which are specific for particular tubulin (Alberts et al., 2008). In this assignment from different cellular examples, these interactions between the specific accessory proteins and the microtubule molecular structure will be examined in order to describe the structures and functions of these specific accessory proteins. Background While these filaments abound in the cytosol of the eukaryotic cells, they have been classified into three categories based on size indicated by their diameter, type of the subunit, and subunit arrangement...
6 Pages(1500 words)Essay

A functional DyneinMicrotubule Network Is Required for NGF signaling through the Rap1MAPK pathway

...rtance of NTs on neurons was one thing; finding out how it is transported to the cell body was another. Shedding light on the problem are the works of Sofroniew, Howe and Mobley (2001) and Ginty and Segal (2002) which both found evidence for the retrograde transport hypothesis. According to the hypothesis, the binding of the nerve growth factor (NGF) to the receptor kinase A (TrkA) activates the latter at the axon (AX) terminal. The NGF -TrkA then enters the cell body thru clathrin-mediated and independent pathways. However, much still has to be learned about the retrograde transport process. In 2003, the role of dynein-based transport of NT signals with the use of intact microtubules was identified by Delcroix and his colleagues. The...
3 Pages(750 words)Lab Report


.... This evidence led the author to explore the role of microtubules depolymerization in the disruptions of apical junction complex. Hypothesis of the study Existing evidence strongly support the role of microtubules depolymerization in the disruption of intercellular connections like tight junctions and adherens junction in various tissues and helps in the pathogenesis of various disease processes. In this regard it has been shown that the endothelial intercellular junctions are also disassembled as well as others like in the brain. Building a rationale on the basis of these data, author formulated a hypothesis: 'microtubules are involved in the disruption...
6 Pages(1500 words)Essay


...romere and each one is attached at each chromatid (Snedden, 1-48). Metaphase: the two centrosomes begin pulling the chromosomes along their attached centromeres towards the end of the cells. The chromosomes become tensed from the longitudinal pull. The centromeres of the chromosomes align along the metaphase plates (McAinsh, 100-330). Anaphase: the proteins that bind the sister chromatids separate and are then pulled apart by the shortening kinetochore microtubules. They then move to the centrosomes to which they are attached. The nonkinetochore microtubules lengthen and pull the centrosomes to the opposite ends (Snedden, 1-48). Telophase: the nonkinetochore microtubules will continue to lengthen in the process elongating the cell even...
1 Pages(250 words)Essay


... tube-like monomer chains. These chains are helical like. α-βtubulin subunits make up the helical tube that has 13 protofilaments that are aligned in a parallel way. The subunits enable the tube-like monomers to link with each other using vertical and lateral bonds. The α-tubulin domain bind to the β-tubulin domain forming these bonds. Hence, the mechanical strength of the microtubules is enhanced. The Microtubule Organizing Centre mediates the nucleation of the microtubules. It comprises a ring complex of γ-TURC and γ-tubulin (Atkinson, 2014, 5870). The specific ring complex serve as the template of the 13 subunits of the microtubules. Microtubules grow from the minus to the positive side. The minus side is located at the centrosome...
7 Pages(1750 words)Essay

Darwin's Black Box The Biochemical Challenge to Evolution

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2 Pages(500 words)Book Report/Review
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