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Enzyme kinetics - Assignment Example

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When the catalytic site becomes empty, more substrate is available to bore and undergo reaction. Vmax is the maximum rate of reaction and denotes the rate of reaction when the…
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Enzyme kinetics
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"Enzyme kinetics"

Download file to see previous pages A competitive inhibitor is a compound that resembles the molecular geometry and the chemical structure of the substance. Such inhibitors compete with the substrate molecule for the same site. We can measure the enzyme inhibitor ki through isothermal titration calorimeter. This involves titrating the inhibitor into a solution of enzyme and then measuring the heat released. Another way of measuring inhibitors is through Michaels Menten equation. Here, we need to observe an enzyme activity under a variety of substrates and concentrations. We can then plot the fitting data on graphs using Line weaver-Burk (Leskovac, ...Download file to see next pagesRead More
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