Molecular biology - Essay Example

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One of the major global burdens of sexually transmitted diseases is gonorrhea, second only to Chlamydia.106 million gonococcal infections have been reported annually, making it a disease that demands immediate attention and treatment. However, during the recent years frequent…
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Molecular biology
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The developing multi drug resistant Neisseria gonorrhoeae, the severe symptoms of the gonococcal infections, added to the socioeconomic burden and epidemiologically threatening aspects of the disease; drug resistance gonorrhea, its detection and diagnosis has acquired immense significance (Blomquist, et al., 2014). It is important to focus on preventing the spread of resistant forms as part of disease management. An essential requirement for controlling spread is enhancing surveillance through better diagnostic methods for identification and isolation of disease resistant pathogens. This project aims to introduce a novel method of diagnosis of drug resistance in clinical isolates of gonorrhea.
The method presented in this paper involves the use of Tiling array for diagnosis of drug resistance in clinical isolates of Neisseria gonorrhoeae. The protocol is based on similar technology already used for development of protocols for drug resistance in other organisms.
Tiling array is a derivation of microarray technology developed by Kapranov and colleagues (2002) and Shoemaker and colleagues (2001) that facilitates identification of previously unidentified transcripts through genome wide annotations.
The initial euphoria associated with the utility and significance of antibacterial and antimicrobial drugs seems to fade with the rise in the bacterial strains exhibiting resistance to single as well as multiple drugs. Hence drug resistance has become an exponentially rising global health hazard rendering world population once again vulnerable to the threats of common diseases (Levy & Marshall, 2004). Both Gram positive and Gram negative bacteria have been known to exhibit multi-drug resistance leaving health care providers with no antimicrobial therapeutic agents ensuring control and management of ...Download file to see next pagesRead More
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