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Biology (medical botany) - Essay Example

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The reason for performing this combinative experiment was also to show how angiogenesis could be inhibited as a major cancer chemotherapy strategy. To…
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Biology (medical botany)
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Download file to see previous pages Several findings and implications of the same are established in this study. It concluded that an inhibition was exhibited in the migration of the endothelial cells of the human umbilical cord, in vitro, due to the use of both drugs. Subsequently, the researchers noted that the result of the migration as synergistically inhibited was due to the combination of the two drugs; thus, implying that the drugs were synergestically related. In another perspective, the drugs indicated independent action modes given that artesunate, in XTT assay, repressed HUVEC growth, while captopril in XTT array did not in the entire range of the dose.
In establishing the quail egg CAN assay, the study established that a significant reduction in the surface area of the vascular chorioallantoic membranes was noted. The artesunate is shown to have an impact on the growth and structure of the blood cells as witnessed in the veins of the eggs that did not turn red. However, the formation of the blood vessels was inhibited by both drugs, significantly, as compared to the DMSO, which was the negative control.
In another finding that analysed the branching of the blood vessels in quail CAM assay, measurements made revealed that the branches fractions and lengths in quail eggs treated by both artesunate and captopril significantly differed from the DMSO with the P < 0.02 at the student’s t-test. Instead, significantly lower junctions’ fractions were recorded in the case of artesunate and not for the captopril quail eggs (Krusche et al., 2013).
Consequently, in testing for the synergism between the captopril and artesunate in vivo, the IC50 values for both drugs were determined for single doses. The effect of this was that a substance that was not affected by the drug, its obtained vascular area was to be 50% of the negative control. If lower than 50%, then the substances acted antagonistically, while if higher than 50%, then the substances acted synergistically. In ...Download file to see next pagesRead More
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