5Sec Rule Exp Report - Essay Example

Section: Bio 100 5 Second Rule Experiment Second Week Introduction 5 second rule experiment second week is an experiment carried out to determine the extent of reproduction and consequent level of threat bacteria cause on nutrients agar and blood agar. This experiment aims at testing the hypothesis Materials and methodsNutrient agar right half, nutrient agar left half, blood agar right half, bologna, gummy bear, blood agar left half, nutrients agar control and blood agar control were used in this experiment.

The experiment was done in five seconds intervals and them measurements were done on the samples to ascertain the level of bacteria in them, type of colony formed and how fatal are the threats they pose.ResultsPLATETOTAL COLONIES# OF DIFFERENT COLONY TYPES BASED ON MORPHOLOGYADDITIONAL RESULTS MORPHOLOGICAL TYPES AND GROWTH PATTERNS (I.E HEMOLYSIS)Nutrients agar right halfBear gummy70% of slide is coveredLobate circular punctiformRaised and roughNutrients agar left halfBologna70% of slide is coveredIrregular lobate punctiformRaised and roughBlood agar right halfGummy bear90% of slide is coveredraised circular irregularBeta hemolytic 60% see throughBlood agar left halfBologna95% of slide is coveredRaised lobate circularBeta hemolytic 45% see throughNutrients agar controlGummy bear 0Bologna 2Gummy bear N/ABologna circularRaised SmoothBlood agar controlGummy 0Bologna 3Gummy N/ABologna circular punctiformGamma hemolyticDiscussionIn nutrients agar bacteria multiply and cover 70% of the slide forming lobate circular punctiform with a raised and rough pattern.

In blood agar along gummy bear they multiply and fill 90% of the slide forming a raised circular irregular colony with a complete hemolytic 60% see through unlike in blood agar along bologna where they fill 95% of the slide forming a raised lobate circular colony with a complete hemolytic 45% see .A control experiment is used for standardization . In conclusion bacteria reproduce well in blood agar and produce more toxic substances than in nutrients agar within a given time. ReferencesZeller, Nancy.

Great Experiments in Biology. 2012. University Readers, Inc.