Calculation for RBC - Lab Report Example

Calculation for RBC Section A Length of one smallest square = 1/20 mm Width of one smallest square = 1/20 mm Depth of one smallest square = 1/10 mm Volume of one smallest square = 1/20 x 1/20 x 1/10 = 1/4000 mm3 Total number of RBCs counted = 1036 (5 squares, 4 corners + 1 center) No. of RBCs in one smallest square = 1036/5 = 207.2 No. of RBCs in 1mm3 undiluted = 207.2 x 10000 = 2072000 / mm3 The Red Blood cell count according to the improved hemocytometer is 2.07 x 106 mm3. According to this reading the person seems to be anemic which may be due to iron deficiency, folate deficiency, genetic disorders or immune dysfunction.

The difference between two readings could be due to many reasons. The high value in the second test can be attributed to inadequate wiping of pipette, improper mixing or uneven distribution in counting chamber. The low value can be due to dilution of contents in pipette by saliva and use of non standard counting chamber and improper cover slip adjustment. Error calculation can result in both high and low values. Even if the second value is correct the women is still anemic. To confirm the findings of both tests a packed cell volume (PCV) or hematocrit test may be done which will give the correct percentage of erythrocyte volume in the blood.