PhIP can accurately identify known and previously unreported autoantigens - Coursework Example

Comments (0) Cite this document
The paper aims to prove that that PhIP can accurately identify known and previously unreported autoantigens. The experimental procedures in figure 3 are shown in the flow chart above. The experiments seek to affirm the hypothesis that “PhIP can accurately identify known…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER91.7% of users find it useful
PhIP can accurately identify known and previously unreported autoantigens
Read TextPreview

Extract of sample "PhIP can accurately identify known and previously unreported autoantigens"

Analysis of the experimental procedures used in figure Analysis of the experimental procedures used in figure 3 The hypothesisof the experiment is “To prove that that PhIP can accurately identify known and previously unreported autoantigens “
Flow chart of experimental program of works
The experimental procedures in figure 3 are shown in the flow chart above. The experiments seek to affirm the hypothesis that “PhIP can accurately identify known and previously unreported autoantigens” figure 3 shows seven figure that represent various experimental processes. This summary shall try to analyze those experimental processes and the key technicalities behind them. Key technical issues in the program of work include the accurate and PhIP sequencing and T7 cloning, verifying and analyzing results from human samples with different consenting PND patients.
Design of T7-Pep, (this is for explanation of fig 3.(c))
Firstly the human protein and cDNA sequences available are downloaded from the database at build 35.1 of the human genome. Then this cDNA and the proteins are used to construct the library.
Cloning of T7-Pep
This procedure involved the proteome wide library and the N/C terminal libraries that were PCR amplified as 23 independent pools with common primer sequences.
This process was conducted in a controlled thermal profile. The polymerase chain reaction was then digested and cloned into the T7FNS2 making numerous copies of each peptide.the cloning process involved the inserting a peptide in the required ORF sequence selected using restriction enzymes.
Patient samples.
This part is an a explanation of the processes involved in fig 3.d, g, f, c, e Patient A, B and C samples are collected from willing patients approved by relevant medical authorities. The samples of cerebrospinal fluids were kept at temperatures of - 80 degrees Celsius before being used. Before the collection of sample the patients have to undergo various neurological evaluations before the samples are collected for confirmation.
Dots blot validation of candidate autoantigens. (Explanation of fig 3 (a) (c)
The complete collection Open reading frames of the genome was generated by the PCR and gateway recombination cloning as a source for testing antoantigen candidates by immunoblot.
Western blotting was used for immunoprecipitation as shown in fig 3(a).
Western blot as fig 3(a) with cerebrospinal fluid (CSF) from all the patients; patient A,B and C was used to validated the candidate autoantigens. There was full staining for the full-length TGIF2LX –GFP that is usually expressed in 293T cells. Individual clones made have to be verified and tittered. In western blot proteins are usually separated by gel electrophoresis, nitrocellulose is the sheet of paper used for transfer of proteins. The blot is usually incubated with a generic protein like milk to absorb to any sticky places on the nitrocellulose before an antibody is then added to the solutions which are protein specific. The antibodies are usually colored to enable observation. The location of autoantigens are then located by incubating it in a substrate that brings out the blot levels that are recorded and analyzed for comparison. The photographed films are then analyzed using computer software such as image J software
From the figure we can conclude that PhIP can accurately identify known and previously unreported autoantigens from the various experimental procedures.
Srivastava, S. (n.d.). A two-parameter generalized Poisson model to improve the analysis of RNA-seq data. Retrieved November 3, 2011, from Read More
Cite this document
  • APA
  • MLA
(“PhIP can accurately identify known and previously unreported Coursework”, n.d.)
PhIP can accurately identify known and previously unreported Coursework. Retrieved from
(PhIP Can Accurately Identify Known and Previously Unreported Coursework)
PhIP Can Accurately Identify Known and Previously Unreported Coursework.
“PhIP Can Accurately Identify Known and Previously Unreported Coursework”, n.d.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document

CHECK THESE SAMPLES OF PhIP can accurately identify known and previously unreported autoantigens

Company analysis of Ooredoo (previously known as Q-tel)

...? Company analysis of Ooredoo (Previously known as Q-tel) Ooredoo: vision, mission ment, goals, and values Ooredoo group is a leading player in the communications industry operating under the parent group Ooredoo Q.S.C. which was formerly Qatar Telecom Company. The parent company is listed in the Qatar stock exchange and the Abu Dhabi stock exchange. The Ooredoo group is a key communication service provider in the region of Middle East, North Africa and South East Asia and caters to around 83 million customers all over the world. The company is headquartered in Doha, Qatar and is growing ambitiously by capturing the opportunities that exist in the emerging market in the world. The Ooredoo Group operates with a wide range of brands... forces,...
12 Pages(3000 words)Research Paper

Nationally Known Leader

...Nationally Known Leader Leadership is a diverse and dynamic concept, and leaders around the world employ various aspects of leadership in the various leadership pursuits they undertake. Effectiveness of leadership is determined individual characteristics and traits that are true to the individual and not any stage managed personalities (Martineau & Hannum 63). An outstanding and world leader in this context is the United States President, Barack Obama. His personality, determination and commitment to the American society serve as a critical representation of his characteristics and traits. Some, if not all his leadership traits can be employed in the fire and emergency services. Obama’s charisma moved...
4 Pages(1000 words)Essay

Unreported conflict : The North Kivu

...acts of sexual violence perpetrated against tens of thousands of girls, women and men, as well. Sexual violence against men is a common tool used as a weapon of war in the North Kivu region. However, since a vast majority of crimes against humanity in North Kivu go unreported, it is difficult to measure the exact extent of the violence. The media continues to play a critical role in hiding the goings on in North Kivu. As mentioned previously, the media fails to report accurately cases of violence in North Kivu, thereby compounding the problem. The media has not provided adequate editorial priority to the violence witnessed in North Kivu (Lemarchand 2009, p. 117). This is primarily...
5 Pages(1250 words)Essay

The Unreported Facts about Building Seven

... impact damage from the collapsing towers. However, the report admits that prior to the 9/11 tragedy, there is minimal record of fire-induced collapse of large fire-protected steel buildings. Apart from this, the report also mentions that the study conducted regarding the structural design and construction features of Building 7 needs to be confirmed by further studies and analysis (Gilsanz, DePaola, Marion & Nelson). Unreported Facts Due to some disconcerting observations with regard to the collapse of Building 7, some have formulated alternative theories. Although some of these have made it through the mainstream news, it can be observed that nagging issues have not been completely covered. For instance, it was mentioned... of the...
2 Pages(500 words)Essay

Identify Books

...Wordless Books Palmer, H. (1961). A Fish Out of Water. Beginner Books. A Fish Out of Water Helen Palmer Illustrator: Helen Palmer Publisher: Beginner Books Publication Date: 1961 Appropriate Age of Grade Level: Grades K-3 Possible Lesson Connection: This book can be used in connection with lectures about caring for animals as well as developing responsibility for others. Summary: A little boy feeds his goldfish, Otto, more than he is supposed to. Otto grows and grows until the pet store owner must use magic to return him to goldfish size. Zion, G. (1956). Harry the Dirty Dog. HarperCollins Title: Harry the Dirty Dog Author: Gene Zion Illustrator: Publisher: HarperCollins Publication Date: 1956 Appropriate Age of Grade...
3 Pages(750 words)Essay

Identify Law

...Identify Law Cellphone and Texting Law Cellphone usage especially while driving has raised concerns among the citizens and the law enforcers because of the potential risk of causing fatal accidents. Due to these concerns, a considerable number of states in the U.S. have enacted laws that limit or entirely ban the usage of cellphone and its associated accessories while driving. The use of a handheld cellphone when driving has been banned in 9 out of the 50 states in the United States. The states that have banned the use of the hand-held cellphone devices include Connecticut, Maryland, New York, Utah, California, Delaware, New Jersey, Oregon, and Washington and the District of Columbia. Of great importance in this study...
4 Pages(1000 words)Research Paper

Known Information about Dolphins Research

... Dolphins What things enter in your mind when you read the word, “Dolphin”? The sea? The aquarium? Theme parks, maybe? Well for some people, dolphins can even mean food. Yes, I was surprised with that information too. However, let’s start with the basics first. The word dolphin came from the Greek word delphus meaning womb. This is understandable because dolphins are cetaceans. Cetaceans are basically whales – and whales are aquatic mammals. So basically, because of its fish-like appearance, dolphins are “fish with wombs”. There are a lot of species of dolphins – almost forty species! They can be large or small and they can be found all over the world. They are carnivores. They eat...
3 Pages(750 words)Essay

Answer 5 questiona thoroughly and accurately used to compare organisms in the current era to those that existed in the past eras. The methods can be used to determine the process of evolution as it involves the study of genetic differences. Non-metric method is more appropriately used to determine ancestry as it maintains the genetic origin of a species. As a result, it accurately gives genetic relation between species separated by long and short periods of evolutions. Question 4 The analysis and dating of cranium is considered one of the best methods of determining the sex of an individual besides the use of the pelvic. Cranium varies in distinctive shape thus making different people to have non-metric features on different bone structures. In...
3 Pages(750 words)Assignment

Identify rock

...Eucrite Meteorite Rock affiliation Eucrite Meteorite Rock A rock is a solid mineral material that forms most of the surfaceof the earth and almost every other planet in the universe. It underlies the soil and oceans. This paper will be looking at the eucrite meteorite rock and outline some of its striking properties. The name Eucrites comes from the Greek work eukritos, which is supposed to mean ‘easily distinguished’. The reason why they are said to be easily recognizable is that they have silicate minerals in them. The minerals are characterized by relatively large grain size (Patchett & Tatsumoto, 1980). The term ‘Eucrite’ has now become obsolete for the igneous rock known as bytownite-gabbro that is formed in the Earth’s crust... . It is...
1 Pages(250 words)Essay

Analysis of previously gathered individual presentation data

...Analysis of previously gathered individual presentation data Submitted by…………………………………………………. Introduction This paper uses 2000 input data set on three presentations which are “any test presentation” international business presentation and presentation on technical literature review. The assessors in the presentation were coded such that 1 denoted male and 2 denoted female. For the three types of presentation, “Any test presentation” was coded as 1, Technical Literature Review was coded as 2 and presentation on International business was coded as 3. The presenters were also coded such that 1 denoted male and 2 denoted female. On that note, this paper reports and discusses various statistical test and comparisons across as...
5 Pages(1250 words)Statistics Project
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Coursework on topic PhIP can accurately identify known and previously unreported autoantigens for FREE!

Contact Us