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Cell Culture - Essay Example

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The following are the steps of protocol to develop a cell line of the selected cell through cell culture:
4) Check the pH of the…
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Cell Culture
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Download file to see previous pages The number of giant cells should be low and fixed (MSUM, n.d.).
The cell line selected for the study is rat coronary artery adventitial fibroblasts (Jenkins et al., 2007). The advantage of this selection is the fact that these cells have an important role in developing arteriosclerosis and plaque development (Jenkins et al., 2007), they divide rapidly and can differentiate into myofibroblasts (Jenkins et al., 2007) so those cells and their role in cardiovascular diseases can also be studied by the same culture instead of culturing the cells separately. Also, the culture permits the sole role of fibroblasts to be studied without the influence of other cells on them (Jenkins et al., 2007). The limitation of this cell line is that they have not been studied much before (Jenkins et al., 2007), so their response to different stimuli has not yet been fully determined (Jenkins et al., 2007); therefore, they might show unexpected behavior during ...Download file to see next pagesRead More
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