StudentShare
Contact Us
Sign In / Sign Up for FREE
Search
Go to advanced search...

Biotechnology techniques - Assignment Example

Comments (0) Cite this document
Summary
Short Answers (Approximately 3 pages) 1. Why do you first denature a plasmid DNA template or a PCR product being used for sequencing? (10 points)  The plasmid DNA is denatured to convert the double stranded DNA molecule into single stranded DNA. This single stranded DNA molecule acts as a template for the sequencing reaction…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER92.4% of users find it useful
Biotechnology techniques
Read TextPreview

Extract of sample "Biotechnology techniques"

Download file to see previous pages Is this statement TRUE or FALSE, explain? (5 points) Electroporation is used to introduce DNA in prokaryotic and eukaryotic cells. This is the general method of introducing the foreign DNA into the desired cells. This method uses the electrical pulses to create a pore in the cell membrane and through these pores the foreign DNA molecule is inserted. 3. What are the 3 main sources of Stem Cells? (10 points) The 3 main sources of stem cells included Bone marrow, Bloodstream or peripheral blood and finally umbilical cord blood from the newborns. Bone marrow particularly those in the pelvis’ bones have rice supply. Blood normally has few stem cells, and when hormone like substances called growth factors are given to individuals, stems cells grow faster and move from the marrow to the blood. Blood left in the placenta and the umbilical cord of the newborns can be retrieved, store and utilized for future.  4. What is meant by humanization of mouse antibodies and what is the need for "humanization"? (10 points) While using therapeutic antibodies or murine antibodies isolated from mice in humans, immunogencity problem arose due to the differing protein sequence. To alleviate this issue, specific regions within a mouse antibody called complementarity determining regions (CDR) are identified and transferred into human variable regions and this process is called humanization. When this is done, it will confer binding and so the resultant humanized antibodies can function without major immunogencity problem.  5. Which technology cannot be used to determine the function of a gene? Explain. (5 points) Stem cell technology cannot be used to study the gene function because, it is a method to produce the entire cell and study the organs and it is not the direct method of analysis of gene function.   6. cDNA microarray technology specifically evaluates the gene expression levels of ONLY microRNA in cells and tissues. Is this statement TRUE or FALSE? Explain? (5 points) No cDNA microarray technology is used for the analysis of all the genes and its function. cDNA technology is used to analyse the functions of known and unknown gene.   7. Which of the following gene expression strategies increase the number of different proteins available to the cell without increasing the total number of genes encoded by the genome?(5 points) Translation is used to increase the number of different proteins in the cell without increasing the total number of genes in the genome. By varying the mRNA translation, the protein synthesis is modified.   8. In the sequence given below, how many EcoRI recognition sites are present? Please circle them. (10 points)      5’-ATGGAATTCCCAATTGAATTCCGGCTGAATTCAAA-3’     3’-TACCTTAAGGGTTAACTTAAGGCCGACTTAAGTTT-5’ There are three EcoRI recognition sites in the given sequences. They are  5’-ATGGAATTCCCAATTGAATTCCGGCTGAATTCAAA-3’     3’-TACCTTAAGGGTTAACTTAAGGCCGACTTAAGTTT-5’ 9. What is meant by the statement, "Complementation screening of genomic eukaryotic clones will not work in E.coli because of absence of splicing machinery?" (10 points) E.coli is a prokaryote. The bacterial systems cannot remove the introns from the mRNA sequences. Complementation screening is the technique used to identify the mutations in the gene of interest. In this method, the selection is based on the capacity of the gene to get back the phenotype. The splicing mechanism is not present in the ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Biotechnology techniques Assignment Example | Topics and Well Written Essays - 1500 words”, n.d.)
Retrieved from https://studentshare.org/biology/1497917-biotechnology-techniques
(Biotechnology Techniques Assignment Example | Topics and Well Written Essays - 1500 Words)
https://studentshare.org/biology/1497917-biotechnology-techniques.
“Biotechnology Techniques Assignment Example | Topics and Well Written Essays - 1500 Words”, n.d. https://studentshare.org/biology/1497917-biotechnology-techniques.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document

CHECK THESE SAMPLES OF Biotechnology techniques

Biotechnology

...I disagree with the ment that agricultural biotechnology is promising and can solve the problem of world hunger. I argue that the risks associated with biotechnology far outweigh its purported benefits. Biotechnology in its simplest and most straightforward definition is the modification of the genetic make-up of natural organisms to serve human use, consumption and exploitation. A popular example of biotechnology is the “golden rice” which involves genetic modification for the purported purpose of introducing more nutrients and vitamins, particularly Vitamin A. The genetic manipulation facilitates the production of carotenoids in the grain’s endosperm, thus giving it...
3 Pages(750 words)Essay

BIOTECHNOLOGY

...Clinical Trials, Pharmacogenomics and the FDA What is a clinical trial? Why do we do them? [Hint: is it simply to show “safety and efficacy” or perhaps something more?]. Clinical trials is a method of clinical research, in which the researcher or researchers who directly observe a person or people, and/or who collect data to answer a scientific or medical question about the safety or benefit of an invention. The invention could be a medicine, a toll, teaching concept, training method, or behavioral change. Clinical trials are done to determine whether the new biomedical or behavioral interventions are safe and effective, whether the human subject invention like the diet, physical activity meets its definition. The trial is also... Trials,...
5 Pages(1250 words)Essay

BIOTECHNOLOGY

...Biotechnology: Week 8 a. Briefly describe angiogenesis? Do you consider angiogenesis to be a form of tissue engineering? Why or why not? Give two examples of how we could use angiogenesis to treat disease, one by turning angiogenesis “on” and a second by turning angiogenesis “off.” According to The Angiogenesis Foundation (2008, pg. 1), angiogenesis is, “the growth of new blood vessels - is an important natural process occurring in the body, both in health and in disease. Angiogenesis occurs in the healthy body for healing wounds and for restoring blood flow to tissues after injury or insult. In females, angiogenesis also occurs during the monthly reproductive cycle (to rebuild the uterus lining, to mature the egg...
2 Pages(500 words)Essay

Biotechnology

...IDENTIFICATION AND DETERMINATION OF ABSORPTION SPECTRA OF CHLOROPHYLL Photosynthesis is a regulated and a multi-step process. It is the process by which the plants produce energy from the sunlight to produce sugar which in turn is used for cellular respiration to produce ATP, the store of energy of the cells. This conversion of the less used energy into a usable chemical energy is assisted by the green pigment called chlorophyll. The photosynthesis uses the water molecule and releases oxygen molecule. (Tanaka and Makino 2009). The pigment is a substance that absorbs light and the color is produced due to the reflection of the light that is not absorbed. Chlorophyll absorbs all wavelengths of the visible light except the green... AND...
5 Pages(1250 words)Essay

Biotechnology

...POSITIONAL ESSAY ON BIOTECHNOLOGY Biotechnology is a widely used term these days. However it has been in practice for quite a while now. We even observe biotechnology in our very own surroundings. For instance the conversion of milk into yogurt is an application of biotechnology so is the fermentation of fruit juices and the use of yeast in baking. These activities have been in practice from ancient times. So no matter how one puts it, biotechnology has been a lucrative field of biology for mankind. Biotechnology is the branch of biology that deals with the application of living organisms and biological processes in aiding and improving...
9 Pages(2250 words)Essay

BIOTECHNOLOGY:

...Biotechnology Pharmaceuticals and/ or biotechnology companies spend billions and take an average of ten to fifteen years to discover and bring a new drug to the public. The drug discovery and development process ensures that only safe and effective pharmaceutical products are brought to the market. Pharmaceutical companies follow similar pathways for the discovery of drugs. Although each company has its own standards and measures for measuring successful drug discovery, the most appropriate measures are ensured to meet safety and effectiveness standards in patients with little compound attrition. Keywords: Pharmaceuticals, Drugs, Drug discovery, Drug development, Pathways, Standards, Measures, Patients,...
8 Pages(2000 words)Research Paper

Biotechnology

... Rhetorical Strategies Used in the Film Gattaca The three rhetorical strategies that Andrew Niccol used in the film Gattacawere logos, ethos and pathos. Logos One of the rhetorical strategies used by Andrew Niccol for the audience to be sympathetic to Vincent is logos which is the use of logic or reason in conveying an idea. The rhetorical strategy logos was obviously used in the film to draw sympathy for Vincent when society put so much emphasis in predetermining the destiny of individual just by his or her genetic make-up. Gattaca’s over reliance on logic puts the audience off because it failed to consider other factors such as persistence, will and discipline that would make an individual effective and successful... Rhetorical Strategies ...
2 Pages(500 words)Movie Review

Biotechnology

...Genetic Engineering Genetic Engineering Genetic Engineering is the one of the promising fields of Biotechnology and it involves themanipulation of the genetic material of an organism in order to produce desired biomolecules from the organism. Human beings attempt to genetically engineer the animals to produce beneficial products or to develop beneficial attributes in the organisms. The primary literature source written by Peter Dickos with the title The Next Frontier In Food: FDA Regulation Of Genetically Engineered Animals explains the various aspects of genetically engineered animals and also the Food and Drug Administration’s (FDA ) regulatory measures that are needed to be brought out for the safe use of GE animal...
2 Pages(500 words)Essay

BIOTECHNOLOGY

...Biotechnology: Cloning Due The idea of cloning has been at the heart of any number of science fiction stories over the course of the last century. However, it is not a fairytale; it is, in fact, a reality. The potential to clone cells, organs, animals, even whole individuals, is no longer considered impossible and could very well be a huge part of the future of science and research. How is cloning accomplished? Well that is entirely dependent upon what one is intending to clone; reproductive, cellular, or organ cloning. Dolly, for example, was created through a process using mammary cells of a donor sheep. Human cloning in its most basic form is the act of taking one individual and making an exact biological replica of...
2 Pages(500 words)Coursework

Biotechnology

...Overview of the Upstream and Downstream Processes for Biopharmaceuticals Real Time Quality Control Overview of the Upstream and Downstream Processes for Biopharmaceuticals Real Time Quality Control The application of biotechnology has opened new realms that have presented opportunities for the large-scale production of drugs. However, the production involves multiple processes that range from the upstream processing and to the downstream processing that ensures that the product undergoes purification to the required degree and eventually packaging. The focus of this essay will be on the upstream and downstream processing, and then discuss the significance of real time quality control and product analysis technology. In a...
8 Pages(2000 words)Essay
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Assignment on topic Biotechnology techniques for FREE!

Contact Us