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Respirstion - Lab Report Example

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Name Institution Instructor Course Date Abstract This experiment aims at investigating aerobic process in the mitochondria. The experiment uses a suspension of mitochondria obtained from ground Lima beans. The experiment is based on the assumption that the mitochondria should continue functioning if they were intact cells…
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Download file to see previous pages The hypothesis for the experiment is that if the substrate concentration of Succinate increases, then the rate of aerobic respiration increases was not confirmed. The conclusion of the experiment was discovered that succinate concentration and enzyme concentration does affect the rate of aerobic respiration. Introduction Aerobic respiration occurs in living things and is a process through which food substances are broken down to release energy in form of ATP. This process takes place in the mitochondria and requires energy. Unlike the process of fermentation where food substrate is broken down in absence of oxygen where only a lesser number of ATP are produced, aerobic respiration results in production more energy in form of ATP. The first part of aerobic respiration (glycolysis) is similar to that of fermentation and takes place in the cytoplasm. Pyruvic acid, a three carbon molecule is formed through a series of reactions on glucose molecules. Two molecules of ATP are released absence of oxygen as anaerobic respiration takes place (Apte et al 37). The pyruvic acid then proceeds to the next stage. C6H12O6 CH3COCOOH (Pyruvic acid) The second phase of aerobic reaction takes place in the matrix of the mitochondria. ...
Objectives To investigate the factors affecting the rate of aerobic respiration Hypothesis If the substrate concentration of Succinate increases, then the rate of aerobic respiration increases. Procedure The materials needed for this experiment includes test tubes, lima beans mitochondrial suspension, succinate, DCPIP, buffer and paraffin, In this experiment, four small test-tubes were obtained and labeled B, 1, 2, 3, 4 test tubes B was blank. In the blank test tube, 4.6 ml of the buffer, 0.1ml of succinate acid and 0.3ml of mitochondria suspension was placed. The tube was then covered with a film of paraffin and inverted to ensure that the contents mixed completely. A micropipette was then used to transfer 1ml of the mixture to a square corvette. The machine was then set to read transmittance. The wavelength of the machine was set at 600nm. The corvette was placed in an open spectrophotometer. The transmitter was set to 100 where the blank was used as the baseline. In tube 1, 2 and 3, DCPIP, mitochondria suspension and the buffer were placed in measurements indicated in the table below. Succinate was not added at first Reagents Tube 1 Tube 2 Tube 3 Buffer 4.4ml 4.3ml 4.2ml DCPIP 0.3ml 0.3ml 0.3ml Mitochondrial suspension 0.3ml 0.3ml 0.3ml Succinate 0ml 0.1ml 0.2ml Succinate was then quickly added to the test tubes which were then covered with paraffin and placed in the corvette. The corvette was wiped on the outside and then placed in the samples holder ensuring that the arrow pointed downwards. The lid of the machine was closed and the readings for each sample recorded at different times. The spectrometer was reset after every five minutes using the blank as the baseline. The content of the ...Download file to see next pagesRead More
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