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Development of Simulated Intestinal Fluids - Research Paper Example

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The paper "Development of Simulated Intestinal Fluids" explores the ideal transport medium containing appropriate nutrients, which could support the Caco-2 cell culture model thereby facilitating the transport of traditionally hydrophobic molecules like estradiol and etoposide…
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Development of Simulated Intestinal Fluids
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Download file to see previous pages The study seems well designed and original. It has built upon previous work on the facilitation of lipophilic drugs solubility on the Caco-2 cell models by incorporation of agents like dimethyl sulfoxide (DMSO), ethanol, cyclodextrins, and surfactants. The reason cited by the authors for this study is the fact that a large number of recently discovered drug molecules and pharmaceuticals are lipophilic. Just by diluting the lipophilic molecules by the agents described above disturbs the normal physiological environ of the in vitro model which has to be as identical to the in vivo conditions as possible, and can lead to misleading interpretations because the normal physiological status in the living cells is entirely different and needs to be emulated precisely to provide accurate analysis in such studies. This factor has been given prime importance by the authors while undertaking this study.
The authors have based the study on past attempts to simulate intestinal fluids artificially by the addition of bile salts, phospholipids, and/or fatty acids. They have analyzed the previous studies well and the paper elaborates upon the various approaches tried and the success or failures thereof in the introduction itself. They have also taken into consideration the altered normal physiology of the digestive tract in the pre and postprandial states. The presence of different levels of digestive enzymes in these two states is an important aspect while developing in vitro models. Simulated Intestinal Fluids (SIFs) with varying compositions were studied for their effect in maintaining cellular integrity, the production of Lactate dehydrogenase in Caco-2 cell cultures, and by measuring the cellular protein content.
The authors have built upon this study based on the study on past attempts to simulate intestinal fluids artificially by the addition of bile salts, phospholipids, and/or fatty acids. They have analyzed the previous studies well and the paper elaborates upon the various approaches tried and the success or failures thereof in the introduction itself. ...Download file to see next pagesRead More
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Development of Simulated Intestinal Fluids Research Paper Example | Topics and Well Written Essays - 1250 words. https://studentshare.org/medical-science/1544368-development-of-simulated-intestinal-fluids-containing-nutrients-as-transport-media-in-the-caco-2-cell-culture-model-assessment-of-cell-viability-monolayer-in
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Development of Simulated Intestinal Fluids Research Paper Example | Topics and Well Written Essays - 1250 Words. https://studentshare.org/medical-science/1544368-development-of-simulated-intestinal-fluids-containing-nutrients-as-transport-media-in-the-caco-2-cell-culture-model-assessment-of-cell-viability-monolayer-in.
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