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Special Stain: Usefulness for Modern Diagnostics Histopathology Laboratory - Essay Example

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HEALTH SCIENCES AND MEDICINE Your Instructor Special Stains Definition Special Stains is a commonly used term in the laboratory settings and is applied in two main areas: diagnostics and research. In research, strains are applied as probes in the identification of selected chemical constituent’s in both abnormal and normal cells…
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Special Stain: Usefulness for Modern Diagnostics Histopathology Laboratory
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Download file to see previous pages Special strains fall under an assorted stains family for general identification and microscopic visualization of tissues, cells and microorganisms. Special stains is an important laboratory tool used by many technologists and pathologists offering a strong complement to flow cytometry, immunohistochemistry, in situ hybridization and diagnostic technologies defining the medical profile of patients. With greater demands from the medical community for quality and standardized control, protocols on special stains have ended up being more automated leading to higher levels of flexibility and productivity. Automation cannot be considered as being a substitute for the basic understanding of the practices and principles of staining that is of high quality. Most people in the medical field anticipate that such technology will keep on growing so as to evolve in the predictable future. This has left many with high expectations that the technology will eventually result in pathologic diagnosis that is integral. Special stains applications fall under histology and biology including; RNA and DNA content determination, determining the mode of actions of hormones, drugs and food addictives that are toxic, metabolic biochemistry, disease process biochemistry, metastatic tumor primary sites, metastatic melanomas identification especially those that are not-pigmented, detection of tumors at its early stages, micro organism identification, Barr bodies identification and cell staining (Tadrous 2011). Usefulness in the Modern Diagnostic Histopathology Laboratory Pathology texts on liver insists on the value of having a variety of special strains as being a routine and not special during the evaluation process. They include; eosin, hamatoxylin, reticulin, trichome, periodic, iron stain and basement membrane. Most consider the trichrome helpful in the identification of the early lesions of perisinusoidal fibrosis and pericentral found in NASH (Simmers 2008). The other types of stains assist in the exclusion of the rest of the diagnostic possibilities. Stains for copper-associated protein and copper itself are also regarded as useful in situations where there are concerns of cholestatic injuries, chronic bile duct or Wilson disease. Often used in special stains, it adds nothing to the overall liver biopsy cost as it is not expensive and if done on time, there will be no tissues wasted through block resurfacing. Luna (1999) asserts that of equal significance in the studies and diagnosis of NAFLD is the histologic criterion. The results acquired from the study of biopsies together with correlations of clinic-pathology highlights the importance of using careful criteria in distinguishing NASH and NAFLED. International hepatopathologists have come up with their own way of diagnosing NASH. The criteria used by this international group illustrate the varying opinions that are common in the field today. However, they both share a common theme which is; any modern diagnosis carried out in a laboratory depends on lesions combination as it were a pattern of injuries and not a specific single lesion. The lesions that are mostly included in histopathology laboratory include, hepatocyte ballooning, steatosis, perisinusoidal fibrosis and lobular inflammation. In pathophysiology and Clinico-pathologic correlations while considering the findings from ...Download file to see next pages Read More
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