Cell Biology ADHESION WRITE UP - Essay Example

Comments (0) Cite this document
Summary
Cell Adhesion Practical Introduction Cell adhesion assays can be used to explore cell-matrix interactions in several different types of cell, including epithelial and tumour cells, and thus assays are very important in all areas of biology and biomedical research…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER92.1% of users find it useful
Cell Biology ADHESION WRITE UP
Read TextPreview

Extract of sample "Cell Biology ADHESION WRITE UP"

Download file to see previous pages Cadherins are found as trans-membrane proteins (Rastogi, 2007). There are four main types of classical cadherins, with the epithelial cadherin playing an important role in the growth of tumours (Asano et al, 2004). Cells possessing cadherins generally adhere to other cells possessing cadherins. By exploring these cell adhesion mechanisms with an assay, it is possible to understand more fully the role of the intracellular matrix and the reactions which occur within it. The assay described below relies on the interesting effects that trypsin has on these cadherin molecules. In the presence of calcium and trypsin, the cadherins are protected from cleavage, but in the presence of a trypsin and EDTA mixture, the cadherins are removed from the cell surface and thus the cells stop aggregation. Results Trypsin is commonly used to detach the adhering cells (Humphries, 2001). Prior to the addition of trypsin, the cells were adhering to each other forming a suspended clump of cells within the wells. However, after the trypsin was added, there was less evidence of adhesion and the cells were in the matrix singularly. After fixing, only the adhering cells were found in the well. This again gave a ‘clumped’ look to the cells. After viewing this, the wells were stained with methylene blue, and this the wells had a typical blue appearance in the top two rows, suggesting normal adhering cells were present. ...
Raw Data 1 2 3 4 5 6 7 8 9 10 11 12 A 0.935 0.685 0.798 0.857 2.35 2.323 2.253 2.523 2.427 2.809 2.58 2.416 B 0.445 0.488 0.632 0.401 2.165 2.13 2.308 1.708 0.774 0.851 1.072 0.814 C 0.028 0.023 0.037 0.028 0.025 0.025 0.025 0.025 0.026 0.025 0.026 0.027 D 0.029 0.031 0.027 0.029 0.028 0.023 0.029 0.026 0.027 0.026 0.035 0.03 E 0.027 0.033 0.028 0.03 0.022 0.013 0.029 0.028 0.032 0.022 0.032 0.03 F 0.028 0.024 0.026 0.028 0.027 0.028 0.022 0.028 0.017 0.014 0.021 0.027 G 0.028 0.032 0.024 0.026 0.037 0.021 0.029 0.017 0.018 0.016 0.024 0.034 H 0.032 0.019 0.025 0.023 0.025 0.014 0.028 0.014 0.018 0.013 0.022 0.025 Graph 1 A1-4 A5-8 A9-12 B1-4 B5-8 B9-12 Mean 0.819 2.362 2.558 0.492 2.078 0.878 Standard Dev. 0.105 0.115 0.183 0.100 0.258 0.133 Graph 2 A1-4 A5-8 A9-12 B1-4 B5-8 B9-12 Mean 0.201 0.370 0.346 0.158 0.325 0.407 Standard Dev. 0.009 0.023 0.067 0.020 0.031 0.021 Discussion ECM Component Preference As was previously discussed, cadhesins are of various different types and thus may have different preferences for the extra-cellular matrix in which they react. In the data above, the cells A1-4 represent matrix 1, A5-8 represent matrix 2, and A9-12 represent matrix 3. The cells B1-4 represent the BSA and B5-8 and B9-12 represent tube 1 and 2 respectively. In this case, the data obtained experimentally shows that there is a definite component preference for matrices 2 and 3, with the first one having a very low absorbance value and thus a very low amount of adhering cells. The sample data also show a similar trend, although the amount of adhering cells in matrix 1 are not so low as in the experimental data. There is another small difference in ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Cell Biology ADHESION WRITE UP Essay Example | Topics and Well Written Essays - 1500 words”, n.d.)
Retrieved from https://studentshare.org/environmental-studies/1422659-cell-biology-adhesion-write-up
(Cell Biology ADHESION WRITE UP Essay Example | Topics and Well Written Essays - 1500 Words)
https://studentshare.org/environmental-studies/1422659-cell-biology-adhesion-write-up.
“Cell Biology ADHESION WRITE UP Essay Example | Topics and Well Written Essays - 1500 Words”, n.d. https://studentshare.org/environmental-studies/1422659-cell-biology-adhesion-write-up.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document

CHECK THESE SAMPLES OF Cell Biology ADHESION WRITE UP

Cell biology

...metalloproteinase inhibitors reduce collagen gel contraction and ?-smooth muscle actin expression by periodontal ligament cells. Journal of Periodontal Research, 44(2), p.266-274. Available at: http://doi.wiley.com/10.1111/j.1600-0765.2008.01127.x [Accessed April 3, 2011]. Han, M. & Steinman, L, 2009. Systems biology for identification of molecular networks in multiple sclerosis. Multiple Sclerosis, 15(5), p.529 -530. Available at: http://msj.sagepub.com/content/15/5/529.short [Accessed April 4, 2011]. van Horssen, J., Dijkstra, C.D. & de Vries, H.E., 2007. The extracellular matrix in multiple sclerosis pathology. Journal of Neurochemistry, 103(4), p.1293-1301. Available at:...
7 Pages(1750 words)Essay

Cell Biology

...., and Harrison, S. C. 1993. Recognition of a high-affinity phosphotyrosyl peptide by the Src homology-2 domain of p56lck. Nature 362, 87–91 Eckhart, A. 1979. Cell, 18, 925-933. Fantl, W. J., Johnson, D. E. and Williams, L. T. (1993) Annu. Rev. Biochem. 62, 453-481 Huang, H., et al. 2008. Defining the Specificity Space of the Human Src Homology 2 Domain. Molecular & Cellular Proteomics 7.4. The American Society for Biochemistry and Molecular Biology, Inc. Hunter, T. 1980. Proc. Natl. Acad. Sci. USA., 77, 1311-1315. Instructor’s Manual Post-Lab Activity. Analyzing and Interpreting student Results. Kazlauskas, A. and Cooper, J. A. (1989) Cell 58, 1121-1133 Koch, A.; Anderson, D.; Moran, M....
7 Pages(1750 words)Essay

Cell Biology

... Submitted] Article: KinsethMA; AnjardC; FullerD; GuizzuntiG; LoomisWF; MalhotraV."The Golgi Associated Protein GRASP is Required for Unconventional Secretion during Development". Cell. 2007; 130(3):524-34. 1.0 Background There have been significant interests regarding the physiological function of the Golgi ReAssembly Stacking Proteins (GRASPs). For example, In Vitro Assay studies conducted by Barr et al (1997) and Shorter et al (1999) have shown that GRASPs have an important role to play in the proper stacking of newly created cisternae. Stacking of cisternae membranes are thought to reflect the mechanism which must be present for the production of enzymes and proteins. Other examinations conducted by scientists... , such as that of...
3 Pages(750 words)Essay

Cell Biology

... 30 October 2006 In Eukaryotes, RNA polymerase I II makes RNAs that become mRNAs. RNA polymerase II 2. The start signal for RNA synthesis is the same / different as the start signal for protein synthesis. same 3. Ribosomes have 3 / 4 specialized binding sites for RNA (other than rRNA). 4 4. In Eukaryotes, the initiator tRNA (MettRNA) is loaded onto the small ribosome before / after the ribosome binds to the promoter. after 5. Eukaryotic /Prokaryotic primary transcript RNA is modified to create mRNA by capping, polyadenylation, and splicing. Eukaryotic 6. Prokaryotic mRNA is monocistronic / polycistronic. polycistronic 7. Genes are found on only one / both strand(s) of the DNA. only one 8. Genes on DNA do / do... 30 October ...
2 Pages(500 words)Essay

Cell Biology

...to downregulate the expression of the histidine biosynthetic enzymes in the presence of ample histidine, since the cell does not need to synthesis histidine under those conditions. B. If HisP functions as a gene activator, would you expect that HisP would bind more tightly or less tightly to the regulatory sequences when histidine levels are low? Explain answer. If HisP functions as a gene activator, I would expect it to bind more tightly to the regulatory sequences under conditions with low levels of histidine. As a gene activator, HisP would function to increase the expression of genes necessary for the biosynthesis of histidine. This would be more necessary and beneficial to the cell under...
2 Pages(500 words)Essay

CELL BIOLOGY

...1. A. The protein takes the default pathway: through the ER, to the Golgi apparatus, to the cell surface. B. The protein fails to enter the ER and remains in the cytoplasm. C. Same as A; the protein enters the default pathway through the ER, Golgi to the cell surface. D. The protein is likely to end up as a single-pass transmembrane protein in the ER. If the hydrophobic core in the signal is preceded by more positively charged amino acids than following it on the carboxyl end, then the orientation of the transmembrane protein will be NCytosol/CER Lumen, otherwise the orientation will be NER Lumen/CCytosol. 2. The hybrid proteins in the experiment belong to two sets. In the first, the amino-terminal end of the thiolase is removed... in many...
2 Pages(500 words)Essay

CELL BIOLOGY

... are required to potentiate the norepinephrine signal, then the effects of norepinephrine would be enhanced. 2. Receipt of extracellular signals can cause changes in cellular physiology. Some changes occur on a very fast time scale (i.e., in less than a second) whereas other changes occur long after the receipt of a signal (i.e., hours). A. Name an intracellular change that could result in a quick change in cell physiology. ⇒phosphorylation/dephosphorylation B. Name an intracellular change that could lead to a slow change in cellular physiology. ⇒Gene expression/protein synthesis C. Explain why the response you named in A results in a quick change while the response you named in B results in a slow change. Phosphorylation... and...
2 Pages(500 words)Essay

CELL BIOLOGY

...A diploid sexually reproducing organism contains five pairs of chromosomes that do not undergo homologous recombination during meiosis. If the chromosomes are distributed independently during meiosis, how many different types of sperm or egg cells can a single individual of this species produce? There are five different types of eggs or sperms. 2 Indicate whether the following are involved in meiosis, mitosis, both, or neither. A. DNA replication ____ both in mitosis & meiosis ___________________ B. Spindle formation ____ both ___________________ C. Bivalent _____ in meiosis __________________ D. Chromosome condensation _____ both __________________ E. Maternal chromosome segregation to one...
2 Pages(500 words)Essay

Cell Biology

...Cell Biology ID Lecturer Cells are the most basic and fundamental units of life for any living organism. Every single function in the bodies of humans, animals and plants depends upon the formation and mobilization of these cells. Scientists and researchers estimate that our bodies comprise of somewhere around 75 to 100 trillion cells. The mechanisms of structuring, energy circulation and reproduction are all based on the formulation of various cells in our bodies. The study of cells is called Cell Biology. The organic cells are of such a small size that they cannot be viewed without a scientific microscope. Their sizes range from 1 to 100 micrometers. Thanks to modern day technology, detailed images of the most complex cells can... be...
2 Pages(500 words)Essay

Cell biology

...in the cell and the process of transfection introduces into a cell an entirely new gene. To discover which cells are expressing a certain transcript of RNA, cell biologists use in situ hybridization. During cell biology, purification of cells can be accomplished using techniques such as flow cytometry, cell fractionation, immunoprecipitation, and so on (Pollard and William, 418). Some of the processes of the cell commonly studied by cell biologists include active and passive transport, adhesion, cell movement, DNA repair...
1 Pages(250 words)Research Paper
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Essay on topic Cell Biology ADHESION WRITE UP for FREE!

Contact Us