Nobody downloaded yet

OXIDATION OF METHIONINE BY SINGLET OXYGEN - Essay Example

Comments (1) Cite this document
Summary
Table of Contents
Abstract 2
Introduction 3
Singlet and triplet oxygen 4
Formation of singlet oxygen 6
Type I and Type II oxidation pathways 8
Photosensitizers 10
Aromatic hydrocarbons and organic dyes 10
Phthalocyanines, porphyrins and other tetrapyroles 14
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER91.1% of users find it useful

Extract of sample
"OXIDATION OF METHIONINE BY SINGLET OXYGEN"

Download file to see previous pages Transition metal complexes as photosensitizers 16
Semiconductors 17
Immobilized photosensitizers 17
Methionine oxidation 18
Determination of singlet oxygen 21
Aims 24
Materials and methods 25
General Information 25
Oxygen measurements 25
Singlet oxygen consumption at different methionine concentrations 26
Singlet oxygen consumption induced by laser. 27
Results and discussion 27
Conclusions 47
References: 49
Abstract
The methionine oxidation reaction using singlet oxygen was studied. Initially this report is focused on presenting the overview of works associated with the topic. Singlet oxygen is described along with the ways it is formed. It was highlighted that singlet oxygen was a more powerful oxidant then triplet oxygen and the types of oxidation pathways are described. The practical aspect of this research is focused on using a photosensitizer, Rose Bengal, to generate singlet oxygen. This is the reason for giving a short overview of photosensitizers is given making focus on the behaviour of Rose Bengal and compounds similar to it in structure. Methionine oxidation mechanism is also described as well as function of oxygen electrode.
In order to research the topic of methionine oxidation by singlet oxygen two experiments were carried out. In the first experiment a series of methionine solutions with different concentrations were prepared. In each case Rose Bengal was added as a photosensitizer. As it was established, the rate of oxygen consumption depends on methionine concentration in the solution being the highest at the lowest concentration of methionine. The process was followed by Michaelis-Menten kinetics therefore the corresponding equations were used to construct Lineweaver-Burke plot and determine the maximum rate of the oxidation reaction as well as the line slope. The second experiment utilised a more vigorous approach. A laser pulse was employed to produce singlet oxygen molecules in the dye containing methionine solution. And by employing various concentrations of methionine and Rose Bengal as photosensitizer construct oxygen consumption plots along with determination of variation of centre-of mass distance and potential energy of the reacting molecules. Methionine oxidation was also described using SPARTAN calculations and the reaction mechanism was presented. This report is initially focused on describing the key literature associated with oxidation of methionine by singlet oxygen, highlighting relevant for the project issues and arguments, then moving on to presenting the research that has been done so far, making accent on the methods used and results achieved, before finally identifying the existing gaps in the study and setting up a plan for future work. Introduction Oxygen was discovered by Joseph Priestley in 1775 (Priestley, 1775). Later, Avagadro described the diatomic nature of oxygen, and the paramagnetic properties of this diatomic gas were studied by Faraday in 1811. Differences between oxygen and other gases such as helium or nitrogen were also investigated (Parkes, 1967). Later in 1928, using molecular orbital theory, it was established that oxygen is paramagnetic due to the presence of the parallel spins of two electrons occupying the outer shell. This form with uncoupled electron pair was named triplet oxygen. Spectroscopy was used to prove the existence of higher energy state, which later was called singlet oxygen (Herzberg, 1934). In the singlet form of this molecule outer-shell electrons are paired in antiparallel spins. Initially, the importance of singlet oxygen was not recognised. It was rediscovered in 1964 in photooxidation experiments and since then became intensively studied. Over the past twenty five years significant increase in data regarding singlet oxygen has led to the ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“OXIDATION OF METHIONINE BY SINGLET OXYGEN Essay - 1”, n.d.)
Retrieved from https://studentshare.org/chemistry/1398394-oxidation-of-methionine-by-singlet-oxygen
(OXIDATION OF METHIONINE BY SINGLET OXYGEN Essay - 1)
https://studentshare.org/chemistry/1398394-oxidation-of-methionine-by-singlet-oxygen.
“OXIDATION OF METHIONINE BY SINGLET OXYGEN Essay - 1”, n.d. https://studentshare.org/chemistry/1398394-oxidation-of-methionine-by-singlet-oxygen.
  • Cited: 0 times
Comments (1)
Click to create a comment or rate a document
wd
wdietrich added comment 6 months ago
Student rated this paper as
This essay was always my weak point. I could never complete it successfully. Still, after I found this particular document, I understood how it should be done. So, I performed my research afterward and completed the essay in several hours, instead of weeks as it was previously.
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Administration of Oxygen Therapy
...therapies are under tremendous criticism though they presume a recommendable job. However, medical doctors in the U.S and Canada who use oxidative therapies have often been persecuted by state medical authorities and medical societies. (Nathaniel, 2007, pg. 1) The effectiveness of the therapies to addressing health issues is not determined. Ozone therapy is not effective or its effectiveness has not been determined regarding these health problems. (Nathaniel, 2007, pg. 89) The qualitative methods have shown that the symptomatic benefits of oxygen in patients with cancer who have nonhypoxic dyspnea are not well defined. (Ann et al, 2006). Address of these gaps from the scientific mode of design research...
13 Pages(3250 words)Research Paper
OXIDATION OF METHIONINE BY SINGLET OXYGEN
...oxygen with the assist of photosensitizers, like Rose Bengal. Such compounds use light to convert triplet into singlet oxygen. Due to low activation energy and its electron configuration singlet oxygen is a highly reactive molecule and can readily form bonds with a number of organic molecules. It is less stable than the normal form of oxygen. However, depending on the environment singlet oxygen has the possibility to exist for more than one hour at room temperature. (Schweitzer, et al., 2003) One of the reactions that singlet oxygen can take part in, is the...
12 Pages(3000 words)Essay
Mice oxygen consumption rate
.... The metabolic rate remains closely related to the rate of ATP production measured by cellular respiration (oxygen consumption or carbon dioxide production. Metabolism represents the set of life-sustaining chemical transformations in the cells of living organisms. Metabolism also refers to all chemical reactions that manifest within living organisms including transport of substances (Cannon and Nedergaard 242). # 3 Factors that affect metabolic rate The metabolic rates can be essentially determined by relatively few factors, namely: temperature, phylogenetic affinities, and mass. The body temperature possesses pervasive impacts on biological processes via the influence of temperature on the activity of metabolically...
4 Pages(1000 words)Essay
Oxygen Saturation
...To investigate the number of fresh water shrimps in relation to oxygen concentration in the river Riber Brook Planning: The aim ofthis study was to see the effect of oxygen saturation on the number of shrimps (gammarus pulex) present within a selected test area. My hypothesis concerns the habitations of aerobic fresh-water species. I believed that as the concentration of dissolved oxygen increases, the number of shrimps present will also increase. My hypothesis was proved by the experiment. Results indicate that a higher oxygen content attracted larger numbers of gammarus pulex. For this investigation my independent variable was the number of shrimps present and the...
20 Pages(5000 words)Lab Report
Oxygen Cascade
...times. Physical activity and other physiological processes, including those affected by disease, may cause oxygen demand to peak well over the average level. The partial pressure of oxygen, on the other hand, may fall off steeply, as at high altitudes. Changes in oxygen availability and demand do not necessarily move in tandem. High altitude sickness is a common example of oxygen demand peaking even as its partial pressure falls steeply. Disease may also affect the supply/demand balance of oxygen at the tissue or cellular levels. There is a complicated and inter-related delivery system for oxygen from the atmosphere external to a...
13 Pages(3250 words)Essay
Animal Oxygen Consumption
...H2O + ATP + heat (Foodstuff) (respiration) (heat) (work) Oxygen consumption was measured by estimating the energy metabolism, since the rate of oxygen consumption as well as energy utilization is generally directly related. In this exercise, the oxygen consumption of Tilapia was determined by using LaMotte Dissolved Oxygen kit in small volumes of water. This technique is titration-based on the oxidizing characteristics of dissolved oxygen (DO). Manganese solution is also added to the tested water samples followed by a strong alkali. Later, the...
8 Pages(2000 words)Lab Report
Biological Oxygen Transport
...Biological Oxygen Transport Oxygen is one of the most important elements to sustain life on this planet. Almost every life form, be it plants, invertebrates and vertebrate animals, require oxygen to carry out essential biological processes. It is crucial for the generation of energy through the oxidation of carbohydrates and other fuel forms. The generated energy is used to carry out important functions including growth and movement in animals. Life of every structural and functional unit of any life form depends upon the availability and utilization of oxygen. Keeping in view the importance of oxygen for life, its transfer in animals and...
6 Pages(1500 words)Essay
Biological Oxygen Transport
.... This positive cooperative binding is because of the conformational changes of the hemoglobin complex; when one unit of hemoglobin molecule binds with oxygen, it induces conformational changes in other subunits of the protein complex and enables other subunits to gain increased affinity for oxygen. Major part of body’s iron is contained in the red blood cells. It is contained in the protein hemoglobin. There are large numbers of molecules in a living body that contain heme. They all function mainly by changing the oxidation states of iron between oxidation state II and III. Iron gives up and takes up electrons when it switched between different...
6 Pages(1500 words)Essay
Diel Oxygen Measurements
... Productivty/respiration ratios on the surface and bottom parts of the San Joaquin Marsh were obtained to determine the gross productivity of the lake. This study was able to find out that certain areas of the marsh have different dissolved oxygen concentrations. Introduction The productivity of aquatic systems is needed in the sustainability of ecosystem. It is habitat of aquatic plants that serve as food for fishes. These fishes, in turn, serve as food for several terrestrial and aerial plants. The aquatic plants, in addition, give off oxygen needed by aquatic animals in their respiration. The water may be used in anthropogenic activity such as cooking and laundry. Measurement of diel changes in dissolved oxygen concentrations... of...
3 Pages(750 words)Lab Report
Oxygen cycle
...Oxygen Cycle Oxygen Cycle Every living being on the planet is a common consumer of oxygen. Oxygen is consumed as the result of respiration process that enables a living being to produce energy by breaking the food for its energy content. This energy is further utilized to perform every day tasks. As, the number of living organism are increasing oxygen would have depleted if there is no constant supply of oxygen. It is constantly produced to provide the demand of oxygen. However, the percentage of oxygen within the atmosphere reduced in the past few decades due to its massive consumption and lacked...
3 Pages(750 words)Essay
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Essay on topic OXIDATION OF METHIONINE BY SINGLET OXYGEN for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us