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Thin Layer Chromatography of lipids - Assignment Example

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The first category is non-phosphorylated lipids and includes cholesterol esters, cholesterol and triglycerides (Nollet & Toldra, 2012). The second category is phospholipids. Phospholipids contain an extremely polar phosphate ester group and…
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Thin Layer Chromatography of lipids
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Download file to see previous pages The material will dissolve in the solvent and move up the solvent front.
(a) A Rf of 1 implies that the compound moved the same distance as the hexane solvent. Therefore, the analyzed compound was highly non-polar because it travelled the same distance as hexane, which is a non-polar solvent.
(b) Ethyl acetate is a polar solvent. Mixing it with hexane will reduce the hydrophobic nature of hexane (Nollet & Toldra, 2012). Consequently, the Rf value of the same compound will reduce since it will not fully dissolve in the mixture of hexane and ethyl acetate.
The TLC system for phospholipase D reaction will include the enzyme itself, phosphatidylcholine and ethyl acetate as the polar solvent. Phospholipase D will hydrolyze the phosphatidylcholine to phosphatidic acid and choline. Choline is more polar compared to phosphatidic acid; hence, it will move further than the acid. Molybdenum spray will be used to monitor the movement of the separated material. At 2 minute, there is little hydrolysis, which then increases to the sixth minute. Standard phosphatidic will be used as a control in the ...Download file to see next pagesRead More
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