Thin Layer Chromatography of lipids - Assignment Example

Thin Layer Chromatography of Lipids Thin Layer Chromatography of Lipids Q G (a) Egg contains two types of lipids. The first category is non-phosphorylated lipids and includes cholesterol esters, cholesterol and triglycerides (Nollet & Toldra, 2012). The second category is phospholipids. Phospholipids contain an extremely polar phosphate ester group and thus it is polar. Therefore, the material that remained at the origin of the egg lanes is likely to be the phospholipid component since it does not dissolve in non-polar solvent. (b) Investigating the nature of the material that remained at the origin in the egg lanes follows the same experimental procedure but with a polar solvent.

The material will dissolve in the solvent and move up the solvent front. Q H (a) A Rf of 1 implies that the compound moved the same distance as the hexane solvent. Therefore, the analyzed compound was highly non-polar because it travelled the same distance as hexane, which is a non-polar solvent. (b) Ethyl acetate is a polar solvent. Mixing it with hexane will reduce the hydrophobic nature of hexane (Nollet & Toldra, 2012). Consequently, the Rf value of the same compound will reduce since it will not fully dissolve in the mixture of hexane and ethyl acetate.

Q I The TLC system for phospholipase D reaction will include the enzyme itself, phosphatidylcholine and ethyl acetate as the polar solvent. Phospholipase D will hydrolyze the phosphatidylcholine to phosphatidic acid and choline. Choline is more polar compared to phosphatidic acid; hence, it will move further than the acid. Molybdenum spray will be used to monitor the movement of the separated material. At 2 minute, there is little hydrolysis, which then increases to the sixth minute. Standard phosphatidic will be used as a control in the experiment.

ReferenceNollet, L. M. L., &Toldra, F. (2012). Handbook of Analysis of Active Compounds in Functional Foods. Boca Raton, Florida: CRC Press.