Choose a pathogen - Assignment Example

The disease burden caused by rotavirus is so huge that intervention needs to be taken. Each year rotavirus causes 114 million episodes of gastroenteritis patients, necessitating primary health care and hospitalization. Studies done recently indicative that 41 % of diarrhea in children is mostly caused by rotavirus. If it is controlled, then a bigger portion of hospitalization cases on diarrhea will be reduced drastically, and subsequent deaths cabbed. To this effect, studies have been done on how the virus can be put into control in causing all this epidemiological diseases.

The vaccines that were introduced have played an integral role in handling the havoc and the capability of this virus to human population diseases. Rotavirus vaccine has been one of the major breakthroughs in the field of medicine especially pediatric medicine (Bhojwani, 2009). The microscopy of rotavirus is usually done under electron microscope staining procedure. The integrity and the result largely depend on the type, pH and the time of the stain. The three aspects must be put into perspective for the positive finding during observation.

Prior to microscopy, the virus must first be isolated, cultured and stained before it can be viewed on the electron microscope. The cultivation of the virus sound technical but also interesting in doing it. The isolation of the virus is done on the stool taken from the human being who is suffering or suspected to have the virus. For example, avian rotavirus strain, CH2, is cultivated in embryonated eggs. It can be done on the yolk sac, amniotic, allantois in combination with membrane of inoculation.

After cultivation, the virus will multiply in huge numbers given the multiplying capability. It is from this inoculated culture of the chicken rotavirus virus that a sample undergoes examination via the electron microscope (Parashar et al., 2009). The virus appears as a wheel where the spikes seen are the virulence factors that the virus uses to