Nobody downloaded yet

Mycobacteria Phagosome Maturation - Book Report/Review Example

Comments (0) Cite this document
The purpose of this research paper is to explore the relationship between the maturation of a phagosome to a phagolysosome and markers (specifically rab5 and rab7, small GTP-binding proteins) acquired during the maturation process from the endosomal compartment (Via et al,…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER93.2% of users find it useful
Mycobacteria Phagosome Maturation
Read TextPreview

Extract of sample
"Mycobacteria Phagosome Maturation"

Download file to see previous pages This research uses a culture of macrophage-like cells (J774) cultured and kept in Cellgro, a medium with appropriate additives. These cells were then inoculated by exposure to the pathogen, M. bovis, incubated, lysed and then prepared for the investigation. After latex bead compartment isolation, flow cytometry and analysis of β-hexosaminidase activity, epiflourescence, laser scanning confocal and electron microscopy, the cells were then analysed for markers and phagosome maturation stage. This was done by gel electrophoresis, GTP-binding overlay assay and Western Blot analysis (Via et al, 1997).
The findings found that initially, the phagosomal cells containing M. bovis BCG had a very similar GTP-binding process as those containing the latex beads (Via et al, 1997). The presence of M. bovis BCG caused an increase in the number of rab5 proteins accumulated by the cell, whereas the presence of the latex beads caused the reverse. Additionally, rab7 cannot be detected in those cells with MPC whereas LPC cells have fluctuating levels of rab 7 throughout the incubation period (Via et al, 1997). This suggests that MPC gains the characteristics of the early endosome (in this case, the presence of rab5 proteins) but can remain isolated from the late endosomal cell characteristics (such as the lack of rab 7 proteins). These results illustrate not only the pattern that phagosome maturation takes but how M. bovis manipulates these maturation events to prevent lysis or expulsion from the host cell (Via et al, 1997).
A study by Kitano, Nakaya, Nakamura, Nagata, & Matsuda. (2008) reinforces the importance of rab5 proteins in the endocytic pathway and the involvement of GTP-binding proteins in the maturation of the phagosome. This research used newly developed imaging techniques to track the rab5 activity in a cell during engulfment of apoptopic thymocytes, finding ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“Mycobacteria Phagosome Maturation Book Report/Review”, n.d.)
Mycobacteria Phagosome Maturation Book Report/Review. Retrieved from https://studentshare.org/biology/1584776-mycobacteria-phagosome-maturation
(Mycobacteria Phagosome Maturation Book Report/Review)
Mycobacteria Phagosome Maturation Book Report/Review. https://studentshare.org/biology/1584776-mycobacteria-phagosome-maturation.
“Mycobacteria Phagosome Maturation Book Report/Review”, n.d. https://studentshare.org/biology/1584776-mycobacteria-phagosome-maturation.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
The Maturation of Emma in Jane Austen's Emma
Emma’s childlike attitudes and behaviours are the products of her upbringing and situation in life. Growing up without a mother, Emma has no boundaries and accountability. The only person who could provide that authority, Mrs. Weston, is so mild that she hardly restrained Emma.
4 Pages(1000 words)Book Report/Review
Novel Discussions-A Farewell to Arms
When Catherine and Frederic meet, Frederic is looking for distraction, adventure, and fun. He is American, yet his sense of adventure has led him to Italy to fight a war. He does not seem opposed to the war he is fighting in Italy’s army, but rather bored by it.
3 Pages(750 words)Book Report/Review
Latin American History
Born in California, in the 1950s, Anderson began reporting for Lima Times in 1979 and throughout the 1980s covered many Central America hot spots (Birnbaum, 2004). His long-time interest in Latin America’s turbulent past and present found its natural expression in the biography of one of the sign figures of this volatile continent, who, being either glorified or repudiated, rose to world fame.
4 Pages(1000 words)Book Report/Review
Anne Moodys Coming of age in Mississippi
Anne Moody has been effective in providing a firsthand version of the experiences by a Black girl's struggles in the background of all-embracing racism that existed in the South in the middle of the twentieth century. The work has been highly acclaimed by the critics and the account by Moody has immense significance as this firsthand account throws ample lights on the general experiences of the African Americans, especially the Black women in the 1950s and 1960s.
4 Pages(1000 words)Book Report/Review
Blood formation and maturation
The synthesis process instigates on the initial day with the development of the proerythroblast that is followed on the second day with the formation of the hemopoietic as the nucleus condense. The third day the polychromatophilic erythroblast
1 Pages(250 words)Assignment
Alice's Adventures in Wonderland
The novel is also popular for its thematic concerns and it incorporates beautiful allusions to the novelist's friends and enemies. The major themes of the novel such as the growth into adulthood and finding of one's identity reflect the situation in the Victorian England.
3 Pages(750 words)Book Report/Review
Under the ribs of death and what it means to be a Canadian
Set in the immigrant community of Winnipeg's North End, Under the Ribs of Death follows the progress of young Sandor Hunyadi as he struggles to cast off his Hungarian background and become a "real Canadian."
7 Pages(1750 words)Book Report/Review
Social Convention
Social convention has always demanded the periods of childhood and adolescence be a time of innocence and as such most parents attempt to shield their children from the harsher realities of life. A child who thinks or behaves like an adult, worries teachers and parents alike.
8 Pages(2000 words)Book Report/Review
Aldous Huxleys Brave New World
Even human beings are produced en masse and conditioned - in neo-Pavlovian style - to emotionless social norms in the Central London Hatchery and Conditioning Center. Among the two main characters, Bernard Marx is the nervous, perplexed and critical Alpha-Plus but John "The Savage", the outsider whose moral disdain, revulsion and fright for the "New World" society leads him to commit suicide.
4 Pages(1000 words)Book Report/Review
Elements of Literature
Not much emphasis is given to the childhood and adolescent years of Amory Blaine. However, the few years of his young adult life that is depicted is a process of maturation and coming of age. Through his romantic
1 Pages(250 words)Book Report/Review
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Book Report/Review on topic Mycobacteria Phagosome Maturation for FREE!
logo footer
Contact us:
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us