Nobody downloaded yet

Microtubules - Essay Example

Comments (0) Cite this document
Author has reviewed extensively the available data for developing a rationale to carry out this research work. This literature review has not only been quantitatively but also qualitatively well chosen to support the current research work. …
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER98.8% of users find it useful
Read TextPreview

Extract of sample

Download file to see previous pages Microtubules

Again, these mechanisms need further exploration but the existing understanding is that reorganization of F-actin causes destabilization of transinteraction between TJ and AJ proteins of the adjacent epithelial cells and in turn initiate AJC internalization.

Microtubules, which are also an important element of intracellular cytoskeleton have also been found to be closely related to the cell movement as a whole or any intracellular structure formation like, vesiscles and their movement. Data support the abundance of microtubules in differentiated renal and intestinal cells and depolymerization of microtubules leading to disruption of the integrity of TJs and Ajs in some tissues like lungs. This evidence led the author to explore the role of microtubules depolymerization in the disruptions of apical junction complex.

Existing evidence strongly support the role of microtubules depolymerization in the disruption of intercellular connections like tight junctions and adherens junction in various tissues and helps in the pathogenesis of various disease processes. In this regard it has been shown that the endothelial intercellular junctions are also disassembled as well as others like in the brain. Building a rationale on the basis of these data, author formulated a hypothesis: ‘microtubules are involved in the disruption of apical junctions in simple epithelial cells’....
'Disassembly of the AJC in calcium depleted SK-CO-15 cells is dependent on microtubule integrity;'
'Inhibition or attenuation of AJC disassembly by microtubule stabilization would indicate the role for dynamic microtubules in the process;'
'Polarity of perijunctional microtubules would dictate the type of microtubule motor which is involved in disassembly and internalization of the AJC.'

To test these hypotheses, the author carried out a research study where a number of experimental activities were carried out, like: use of antibodies for the detection of intercellular junctions, while microtubules were detected by immunoflurescence labeling and Western Blotting. Cell culture was used was SK-CO-15, which is a transformed human colonic epithelial cell line. To make the medium free from Ca++, the monolayers of epithelium were washed with calcium free-Eagle's minimum essential medium for suspension culture. Pharmacological agents utilized were; nocodazole; docetaxel and pacitaxel; adenylimidodiphosphate (AMP-PNP) and aurintriccarboxyylic acid (ATA).

Major findings
Through this experiment, the author has been able to demonstrate the role of microtubules depolymerization in the disassembly of junctional complexes through a variety of experimental evidence.

Disassembly of the AJC in calcium depleted SK-CO-15 cells

To answer the research question related to first sub-hypothesis, the findings have been:
At normal Ca++ concentrations, depolymerized microtubules with nocodazole TJ and AJ proteins were found to be located at the cell-cell contacts, reflecting chicken-wire staining pattern while in the environment with depletion of Ca++ for one hour the result was disruption of AJs and TJs with accumulation of e-cadherin, occluding and ZO-1 in ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“Microtubules Essay Example | Topics and Well Written Essays - 1500 words”, n.d.)
Microtubules Essay Example | Topics and Well Written Essays - 1500 words. Retrieved from https://studentshare.org/biology/1534501-microtubules
(Microtubules Essay Example | Topics and Well Written Essays - 1500 Words)
Microtubules Essay Example | Topics and Well Written Essays - 1500 Words. https://studentshare.org/biology/1534501-microtubules.
“Microtubules Essay Example | Topics and Well Written Essays - 1500 Words”, n.d. https://studentshare.org/biology/1534501-microtubules.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
Immunolocalization of the microtubule cytoskeleton
Determining the location of the already known proteins may also be helpful in understanding disease mechanisms and developing new drugs. The importance of knowing the location of a specific protein led to the creation of several methods in detecting proteins.
5 Pages(1250 words)Essay
No topic
The cytoskeleton and its network in particular have integral roles in maintaining the cell’s shape, facilitating in cytoplasmic streaming, as well as in the movement and arrangement of the organelles within the cell especially during cell division (Galjart, 2010).
4 Pages(1000 words)Essay
In byline, the author of the article, Daniel Needleman, has said that experimentation has been able to understand the difference that exists in this cell division phenomenon among the animals. This difference is found out to reside on the chromosomal and molecular level rather on higher level view.
4 Pages(1000 words)Essay
By using indirect immunofluorescent labeling transient transfection of EGFP-Tubline/EGFP-CLIP-170 plasmid to epithelial cells lines and time-lapse microscop,to show that EB1 and CLIP-170 doesn’t bind only to
7 Pages(1750 words)Essay
Organisation of the body
Ribosomes read mRNA code from the DNA in the nucleus and synthesize new corresponding proteins. Its main function is, thus, to synthesize proteins. This is a complex of
7 Pages(1750 words)Essay
Chemically, microtubules are the class of fibrous proteins formed within a cell. These long, linear, and stiff polymers abound throughout the cytoplasm and are the governing structures that control the organization and function of membrane-bound organelles and other relevant components within the cell.
6 Pages(1500 words)Essay
Theory of Endosymbiosis
This theory is most efficient in being able to explain the origin of chloroplasts (in this case a photosynthetic prokaryote such as a cyanobacterium would be the endosymbiont) as well as mitochondria (perhaps an aerobic heterotroph). The progenitor of the mitochondria or chloroplast could have gained entry into a host prokaryote as undigested prey or as an internal parasite after which the "arrangement" quickly became mutually beneficial to both partners (the "host" cell and the endosymbiont).
4 Pages(1000 words)Essay
Cell Structure
The macromolecular components of the prokaryotic cell are relatively basic compared to their eukaryotic counterparts. Eukaryotic cells in contrast are much more complex and take a longer time to replicate during cellular division. One defining feature of the eukaryotic cell type is the presence of abundant organelles, microscopic structures which serve as partitioned areas of specific activity within a cell (Prokaryotic and Eukaryotic Cells, 2000).
2 Pages(500 words)Essay
ASSIGNMENTbiologychemical and cellular foundations of life
In the diagram the glycoproteins and the glycolipids are on the X side, which is the cell surface side since, glycoproteins and glycolipids are cell surface receptors and also plays a role in cell adhesion. Thus, the Y side is cytoplasm of the cell. Integralproteins or transmembrane proteins- Span the membrane and have a hydrophilic cytosolic domain which interacts with internal molecules, a hydrophobic membrane-spanning domain which anchors it within the cell membrane, and a hydrophilic extracellular domain which interacts with external molecules.
6 Pages(1500 words)Essay
The two processes define the mitotic phase of the cell cycle that divides the mother cell into two daughter cells that are identical (Snedden, 1-48). Mitosis is a complex process that takes place only in
1 Pages(250 words)Essay
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Essay on topic Microtubules for FREE!
logo footer
Contact us:
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us