StudentShare
Contact Us
Sign In / Sign Up for FREE
Search
Go to advanced search...

Microtubules - Essay Example

Comments (0) Cite this document
Summary
Author has reviewed extensively the available data for developing a rationale to carry out this research work. This literature review has not only been quantitatively but also qualitatively well chosen to support the current research work. …
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER98.2% of users find it useful
Microtubules
Read TextPreview

Extract of sample "Microtubules"

Download file to see previous pages Microtubules

Again, these mechanisms need further exploration but the existing understanding is that reorganization of F-actin causes destabilization of transinteraction between TJ and AJ proteins of the adjacent epithelial cells and in turn initiate AJC internalization.

Microtubules, which are also an important element of intracellular cytoskeleton have also been found to be closely related to the cell movement as a whole or any intracellular structure formation like, vesiscles and their movement. Data support the abundance of microtubules in differentiated renal and intestinal cells and depolymerization of microtubules leading to disruption of the integrity of TJs and Ajs in some tissues like lungs. This evidence led the author to explore the role of microtubules depolymerization in the disruptions of apical junction complex.

Existing evidence strongly support the role of microtubules depolymerization in the disruption of intercellular connections like tight junctions and adherens junction in various tissues and helps in the pathogenesis of various disease processes. In this regard it has been shown that the endothelial intercellular junctions are also disassembled as well as others like in the brain. Building a rationale on the basis of these data, author formulated a hypothesis: ‘microtubules are involved in the disruption of apical junctions in simple epithelial cells’....
'Disassembly of the AJC in calcium depleted SK-CO-15 cells is dependent on microtubule integrity;'
'Inhibition or attenuation of AJC disassembly by microtubule stabilization would indicate the role for dynamic microtubules in the process;'
'Polarity of perijunctional microtubules would dictate the type of microtubule motor which is involved in disassembly and internalization of the AJC.'
To test these hypotheses, the author carried out a research study where a number of experimental activities were carried out, like: use of antibodies for the detection of intercellular junctions, while microtubules were detected by immunoflurescence labeling and Western Blotting. Cell culture was used was SK-CO-15, which is a transformed human colonic epithelial cell line. To make the medium free from Ca++, the monolayers of epithelium were washed with calcium free-Eagle's minimum essential medium for suspension culture. Pharmacological agents utilized were; nocodazole; docetaxel and pacitaxel; adenylimidodiphosphate (AMP-PNP) and aurintriccarboxyylic acid (ATA).
Major findings
Through this experiment, the author has been able to demonstrate the role of microtubules depolymerization in the disassembly of junctional complexes through a variety of experimental evidence.
Disassembly of the AJC in calcium depleted SK-CO-15 cells
To answer the research question related to first sub-hypothesis, the findings have been:
At normal Ca++ concentrations, depolymerized microtubules with nocodazole TJ and AJ proteins were found to be located at the cell-cell contacts, reflecting chicken-wire staining pattern while in the environment with depletion of Ca++ for one hour the result was disruption of AJs and TJs with accumulation of e-cadherin, occluding and ZO-1 in ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Microtubules Essay Example | Topics and Well Written Essays - 1500 words”, n.d.)
Microtubules Essay Example | Topics and Well Written Essays - 1500 words. Retrieved from https://studentshare.org/biology/1534501-microtubules
(Microtubules Essay Example | Topics and Well Written Essays - 1500 Words)
Microtubules Essay Example | Topics and Well Written Essays - 1500 Words. https://studentshare.org/biology/1534501-microtubules.
“Microtubules Essay Example | Topics and Well Written Essays - 1500 Words”, n.d. https://studentshare.org/biology/1534501-microtubules.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document

CHECK THESE SAMPLES OF Microtubules

Community Corrections and Re-entry

3 Pages(750 words)Article

Molecular biology of the neuronal cytoskeleton

...? [Teacher’s Biology Molecular biology of the neuronal cytoskeleton. The cytoskeleton is a key defining component in eukaryotic cells. Most people refer to the cytoskeleton as a single component; however, it is comprised of three fundamental structures (Wayne and Morris, n.d). These include the microtubules, microfilaments and neurofilaments, they have different functions, but maintain interaction with one another (Brady and Kirkpatrick, 1). These fibrous subcomponents are synthesized from the polymerization of certain protein subunits. They each have different protein subunit compositions which are determined by the cell type or sub domain (Brady and Kirkpatrick, 1). These structures play a fundamental role in...
3 Pages(750 words)Article

Immuno-labelling, fluorescence microscopy and live time-lapse imaging

...? Immuno-Labelling, Fluorescence and Live Time-Lapse Imaging This paper seeks to explore the cellular changes in the tracheal and lung cell. The changes are found to be influenced by the presence of proteins in the cell sub-tissues and organelles. The detection of the antibody reaction with the specific antigens is examined and all the physiological process behind it. The experimentation processes of immune-imaging of the cells on the photon of the fluorescent microscopy are also explored in the paper. The background of the alterations in the cell behaviour to include shape and movement is examined in the paper. The paper seeks to explain the significance of the microtubules in the cell, which is to increase the motility...
8 Pages(2000 words)Lab Report

Immunolocalization of the microtubule cytoskeleton

...Immunolocalization of the microtubule cytoskeleton - Introduction Proteins are probably the most diverse substances found in the cells found in both the single and multicellular organisms. Estimated around 10 000 different proteins, each protein has its specific function. Cellular functions are often localized in specific compartments of the cell, and being able to localize the unknown proteins may provide important information in determining the function of the proteins. Determining the location of the already known proteins may also be helpful in understanding disease mechanisms and developing new drugs. The importance of knowing the location of a specific protein led to the creation of several methods in detecting...
5 Pages(1250 words)Essay

Microtubules

...Using specific examples, describe the structure and function of the accessory proteins which interact with microtubules Introduction Chemically microtubules are class of fibrous proteins formed within a cell. These long, linear, and stiff polymers abound throughout the cytoplasm and are the governing structures that control the organization and function of membrane-bound organelles and other relevant components within the cell. At the molecular level, these comprise of tubulin molecules, which in turn are a heterodimer comprising of globular polypeptides, alpha and beta tubulin which are closely related and tightly linked. Many cellular functions are controlled by these microtubules which...
6 Pages(1500 words)Essay

The Elderly and Medications

6 Pages(1500 words)Essay

A functional DyneinMicrotubule Network Is Required for NGF signaling through the Rap1MAPK pathway

... Submitted] A Functional Dynein-Microtubule Network Is Required for NGF Signaling Through the Rap1/MAPK Pathway: A Review 1.0 Background Neurons are known to be the receptors and conveyors of signals throughout our body. Such an important role gives rise to the interest in the processes present in that particular kind of cell. One of the processes that have gotten much attention is the mechanism which governs the transport of neurotrophin (NT) to the neurons. NTs have been proven to be essential to the survival and proper function of the neurons by the works of many studies such as that of Campenot and MacInnis (2004) which have identified that the absence of NTs results to destruction of the cell body. Establishing... the...
3 Pages(750 words)Lab Report

Mitosis

...). Interphase: here the cell prepares for cell division by manufacturing proteins and cytoplasmic organelles. This phase is divided into three stages, first gap (G1), synthesis(S) and second gap (G2). The last second phase is where chromosomes replicate (McAinsh, 100-330).. Prophase: the chromatin shortens into a chromosome. Since at the S phase the genetic material duplicated, the replicated chromosomes have two sister chromatids that are bound together at the center. At this stage the centrosomes (coordinating center for a cell microtubules) are pushed to the opposite sides of the cell (McAinsh, 100-330). Prometaphase: the nuclei envelop disassembles and microtubules come in the nuclei space. Each...
1 Pages(250 words)Essay

Microtubules

...Table of Contents 3 Introduction 4 The structure of Microtubule 4 Dynamics of the Microtubule 4 Functions of the Microtubule 6 Materials 7 Methods 8 Quantitative analysis 9 Results and discussion 9 Microtubule dynamics 10 Function of EB1 and CLIP 11 As shown by the video recorded, the accumulation of the CLIP was high at MT plus ends in the control cell as compared to cells with depleted EB1. This insinuates that Clip accumulation is dependent on EB1 presence, especially with the acidic tail in question. The binding action of EB1 can be contributed to its structure, which consists of four-helix bundle and ta dimeric parallel coiled coil (Nakamura et al, 2014, 1060). This...
11 Pages(2750 words)Lab Report

Microtubules

...December 4 Microtubules are made up of monomer chains that have a helical shape. They arecomposed of subunits. They have a propensity for dynamic instability. Hence, they have a characteristic random growth nature. Various aspects spoil microtubules such as the rate of dehydrolisation. They play a key role in mitotic cell division and in the cell itself. By using indirect immunofluorescent labeling transient transfection of EGFP-Tubline/EGFP-CLIP-170 plasmid to epithelial cells lines and time-lapse microscop,to show that EB1 and CLIP-170 doesn’t bind only to plus end microtubules but also important in the MT organization and structural instability. Introduction The structure of...
7 Pages(1750 words)Essay
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Essay on topic Microtubules for FREE!

Contact Us