Potential Causes of Blackout Syndrome - Essay Example

The potential causes of the disease are the existence of bacteria in their bodies that is brought about by food poisoning. The bacterium most probably is a gram-negative bacterium. A study/test is conducted on two types of milk: milk A which causes bleeding in people and milk B which has no effect on people. This test is carried out to determine the contents of the milk that cause this kind of bleeding known as the blackout syndrome.

There were various tests that were done on the two kinds of milk that were provided. They all have different objectives that will help in finding the cause of the black syndrome. The filter test is used to show the different types of microns that are contained in the middle; the Petri dish test is used to culture the milk for more studies; the atomic absorption flame photometer detects the presence and concentration of certain ions; the microscope test helps in the identification of the various shapes of the pathogens.

The tests have different procedures, to culture the milk pathogens it is required to sterilize a nichrome loop, dip it into the liquid being tested and smear it across the agar in the petri dish. Repeat the same procedure on what is to be compared with and wait for the pathogens to culture. The atomic absorption flame photometer takes a certain procedure which as follows: prepare a solution of the substance to be tested, evaporate the solution to leave fine solid particles, and burn the solid particles in the machine to produce a black strip with several distinct colors. For the microscope test take the following procedure: take a small sample of the substance to be tested and add crystal violet to stain the cell, add iodine to fix the purple stain in some types of bacteria, and wash everything in alcohol to decolorize the non-fixed bacteria, add a red counterstain and prepare the sample for viewing. The filtration test requires one to pour the substance to be tested into the machine and turn it on and wait for the machine to categorize the components of the test substance.

The filter divides the components of the milk into three categories which shows the presence of different types of microns in the milk. Burning samples of the test product in the atomic absorption flame photometer show a black strip with several distinct colors. The petri dish test shows culturing in the milk sample in the dish. The microscope test helps to identify the shapes of the different types of bacteria in the milk.

The filtration test shows the presence of fungi indicated by a part of the milk that did not go into the 10 microns ending up in the F-100 bottle, the presence of bacteria indicated by the part of milk that has not gone through the half-micron filter ending up in the F-five bottle. The culturing of pathogens in the petri dish indicates the presence of the bacterium in the milk sample as indicated by the white pathogen growing in sample milk A. The different shapes of bacteria shown through the microscope show the types of bacterium in the milk (gram-positive or gram-negative) and their various colors. The results from the atomic absorption photometer indicate no poison in the milk since the readouts do not indicate anything out of normal.

The filtration process indicates the different types of pathogens in the milk with regard to their sizes. The presence of the sample milk in bottle F100 indicates the presence of fungi while the presence of part of the sample milk in bottle F5 indicates the presence of bacteria and the presence of sample milk in bottle F0 indicates the presence of viruses and chemicals in the milk. The identical coloration of the black strip in the flame photometer shows the normal concentration of salts in the milk thus no poisoning while the growth of pathogens in the petri dish indicates the presence of fungi and bacterium. The textbook indicated that fungi culture in Petri dishes to a lesser extent as compared to bacterium and this helped to rule out the existence of fungi in the milk. The observation of the shapes and colors of the bacteria in the milk under the microscope helps in identifying the disease-causing bacteria in the milk. Research indicates that the bacterium is a gram-negative bacterium since it cultures in the presence of an antibiotic meant to kill the gram-positive bacterium.