We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Nobody downloaded yet

Fast protein liquid chromatography (FPLC) - Term Paper Example

Comments (0) Cite this document
Summary
Fast Protein Liquid Chromatography Background Chromatography is a method of separation utilized in laboratories for separation of chemicals dissolved within the same solvent. This is otherwise known as a mixture. It was developed by a Russian-Italian scientist (Mikhail Tsvet) in 1900s…
Download full paperFile format: .doc, available for editing
Polish This Essay91.3% of users find it useful
Fast protein liquid chromatography (FPLC)
Read TextPreview

Extract of sample
"Fast protein liquid chromatography (FPLC)"

Download file to see previous pages Furthermore, it supposes that the chromatographic column contains an infinite number of separate layers (theoretical plates). Separate equilibrations of the sample between the stationary and mobile phase occur in these layers. The analyte moves down the column by transfer of equilibrated mobile phase from one ‘plate’ to the next. There is a more convincing theory, ‘the rate theory.’ This theory depends on the speed of elution and thus speeds of diffusion of the dissolved particles. The analysis and application of this theory leads to the Van Deemter equation. This equation relates the variance per unit length of a separation column to the linear mobile phase velocity by considering several factors. They are physical, kinetic, and thermodynamic properties of a separation. The physical factors are such as; A) Eddy diffusion. B) Longitudinal diffusion C) Resistance to mass transfer It (chromatography) is thus seen to exploit the differences in partitioning behavior between a mobile phase and a stationary phase to separate the components in a mixture. These components contained within a mixture may interact with the stationary phase based on charge, differing solubility or adsorption capability. Several terminologies are associated with the process of chromatography; a) The analyte- this is the substance to be separated during chromatography. b) Bonded phase- this is a stationary phase that is covalently bonded to the support particles or to the inside wall of the tube being utilized.. c) A chromatogram is the visual output of the chromatograph. d) The eluate is the mobile phase that is leaving the separation column. e) The eluent is the solvent that carries/dissolves the analyte. f) The immobilized phase is a stationary phase that is immobilized on the support particles, or on the inner wall of the column tubing. It is similar to the bonded phase g) The mobile phase is the phase that moves in a definite direction. h) The solute refers to the sample components in a solvent. i) The solvent refers to any substance capable of solubilizing another substance. This is important especially in the liquid mobile phase in liquid chromatography. Several methods of chromatography exist as well (singh). They include; 1) Chiral chromatography 2) Countercurrent chromatography 3) Pyrolysis gas chromatography 4) Simulated moving bed chromatography 5) Reversed phase chromatography 6) Two dimensional chromatography 7) Expanded bed adsorption chromatography 8) Size exclusion chromatography 9) Ion exchange chromatography 10) Supercritical fluid chromatography 11) FPLC The FPLC is the method of interest in this case. The FPLC method was developed and marketed in Sweden by the Pharmacia Company in 1982. It was originally called fast performance liquid chromatography. Principle of functioning The purpose of purifying proteins with FPLC is to deliver quantities of the target protein at sufficient purity. This is done in a way that ensures the protein is in a biologically active state to suit its further use. Furthermore this can mean pure enough that the biological activity of the target is retained. This high level of purity requires preliminary preparation of the sample. This is mostly by IEC. In most FPLC systems, there are two solvents/ buffers (A, B). There is also a resin that is chosen so that the protein of interest will bind to it by a charge interaction. When the sample and mix of buffer (100% A) and protein is introduced, the protein will bind to ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Fast protein liquid chromatography (FPLC) Term Paper”, n.d.)
Retrieved de https://studentshare.org/biology/1473424-fast-protein-liquid-chromatography-fplc
(Fast Protein Liquid Chromatography (FPLC) Term Paper)
https://studentshare.org/biology/1473424-fast-protein-liquid-chromatography-fplc.
“Fast Protein Liquid Chromatography (FPLC) Term Paper”, n.d. https://studentshare.org/biology/1473424-fast-protein-liquid-chromatography-fplc.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Fast Food Restaurant Business Plan
Marketing is a full time dedicated task which involves complicated processes depending upon the nature of business. Along with dedication, it requires preparation, money and time to execute. In order to comprehend the problems with instant solution, one must go to make an effective marketing plan because it will enable him/her to use its time, money, and related assets in productive way.
14 Pages(3500 words)Term Paper
Protein purification using anion exchange membranes
The bound proteins can then be eluted from the stationary phase by changing the pH of the eluted buffer, thereby weakening the electrostatic forces. More specifically, in the case of cation exchange chromatography, raising the pH of the mobile phase will cause the protein molecule to become less protonated and hence less positively charged.
4 Pages(1000 words)Term Paper
KFC and the global fast food industry
It comprises of strengths, weaknesses, opportunities and threats – both internal and external to the corporation. SWOT analysis gives a bird eye view of industry, market and company’s competitors. It helps organizations in formulating a market strategy, which will in long run be beneficial for the respective company (Bohm, 2009).
9 Pages(2250 words)Term Paper
Why Is China Growing So Fast
Why is China growing so fast? Economies play a vital role in the prosperity and development of any country. It refers to a system or network of manufacture and consumption of resources by the labor in any country. The exchange of goods between supplier, consumer, and services provided to the people make basic infrastructure of a country’s economy.
5 Pages(1250 words)Term Paper
Fast Paced Financial
The Light Weight Directory Access Protocol is a communication standard that provides a communication channel for the clients within a directory service. A comprehensive definition of LDAP is defined as “Lightweight Directory Access Protocol, a protocol which helps find people, computers and other resources on a network.
8 Pages(2000 words)Term Paper
Gas Chromatography
The carrier gases can be helium which is an inert gas and nitrogen which is an un-reactive gas. The stationary phase is characterized by a column whose layer of liquid is microscopic or polymer found inside a metal tube or glass. Gas chromatograph is an instrument used to perform gas chromatography.
7 Pages(1750 words)Term Paper
Fast food: Are we taking it too far by blaming fast food restaurants for obesity
This essay focuses on the role of fast food in obesity in the United States. The discussion begins with statistics that demonstrate obesity is increasing in the Untied States. Then consideration of the link between fast food and obesity is examined. Statistics indicates that the obesity rate amongst children and young adults is dangerously high.
4 Pages(1000 words)Term Paper
Drug Development and Toxicology (High-performance liquid chromatography)
g the effects of epoxide hydrolase outside the body environment, it is observed that epoxide hydrolase is able to conevert benzene oxide to hydroquinone and phenol metabolites. Epoxide hydrolase also catalyzes the hydrolysis of xenobiotic epoxides forming vicinal dihydrodiol. It
7 Pages(1750 words)Essay
Drug Development and Toxicology (High-performance liquid chromatography)
The retention times used were of the same range, but the resulting absorbance differ with varying analyte concentrations and peak areas. Relating the Standard samples
7 Pages(1750 words)Essay
Fast cat
These objectives include reduced labor costs, talent retention and complying with the labor laws. This report is aimed to investigate how job structures set to meet the FastCat’s
2 Pages(500 words)Term Paper
Let us find you another Term Paper on topic Fast protein liquid chromatography (FPLC) for FREE!
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us