Nobody downloaded yet

PV92 practical - Assignment Example

Comments (0) Cite this document
Summary
Practical Techniques in Genomics and Proteomics Name Institution Respond to Question 1. It is importance to Chelate metal ions from solution in the process of boiling step at 100 degrees Celsius because chelating agents are chemicals that often form complex, and soluble, molecules with specific metal ions (Ashmead & DeWayne, 1993)…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER93% of users find it useful
PV92 practical
Read TextPreview

Extract of sample "PV92 practical"

Download file to see previous pages This would, therefore, mean that the genomic DNA is not protected from degradation by the cellular enzymes released during the isolation protocol of the DNA. Response to Question 2. In order to carry out a PCR, one requires DNA. This means a cell is important since it is the source of the DNA. The cell can also serve as a source for base pairs, enzymes and primers. Response to Question 3. There are various structures that are often broken in order to release the DNA from a cell. These include the cells, the cheek tissues, the phosholids, the nucleus, and proteinase K. this is done so that proteins along with other organically-soluble compounds are removed from chromosomes and cells. PCR Amplication. Response to question 1. It is important to have a primer on either side of the segment of a DNA that is going to be amplified because these form the four basic bases. Uracil exists in RNA, despite being a special case. Primers are vital and essential components of the synthesis. They synthesize an RNA short piece which is complementary to the strant of the template DNA forming the bonds of hydrogen with it. This provides a DNA polymer with a starting poing which that is required for the synthesis. After the completion of the DNA synthesis, the segment of RNA is removed and a DNA replaces it. Response to Question 2. Taq DNA polymerase is the thermostable DNA polymerase that derived its name from the thermophilic bacterium called Thermus aquaticus. The T.aquaticus is a specific bacterium found in hydrothermal and hot springs vents. Response to Question 3. It is because they are the only four basic bases with uracil being is present in the RNA, although it is a special case. Components of Master mix include water, oug template, the buffer, deoxynucleotides, aum primers, template DNA and ampliTaq polymerase. The buffer is used for keeping the master mix at a suitable PH in order to make sure the reaction of the PCR occurs. Deoxynu-cleotides, in this case help in providing the energy and the nucleosides used for synthesizing the DNA. As widely cited, it is vital adding equal amounts of each of the nucleotide (, dTTP, dCTP, dATP and dGTP) onto the master matrix for purposes of preventing the mismatch of bases. The ouM primers are short pieces of the DNA that often bind onto the DNA template to allow the Taq DNA polymerase enzyme to start the incorporation of deoxynucleotides. The AmpliTaq polymerase is used, in this case, to add the deoxynucleotides onto the DNA template. Last but not least, there is presence of oug template that is often amplified by a PCR reaction. Response to Question 4. The DNA PCR amplification takes place in repeated cycles of the three temperature-dependent steps. In the first step, a double-stranded DNA or (dsDNA) template becomes denatured. In the second stage, the oligonucleotide primers become annealed to a single-stranded DNA also called (ssDNA) template. In this case one primer become designed specifically to anneal onto a certain specific region found on the left side of the strands of the DNA with the other primer being designed specifically to anneal onto a specific region of the complementary DNA strand right side. In the third stage, the DNA polymerase is meant to extend on each primer in 3' to 5' direction, while duplicating the fragment of the DNA between the primers. While ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“PV92 practical Assignment Example | Topics and Well Written Essays - 1750 words”, n.d.)
PV92 practical Assignment Example | Topics and Well Written Essays - 1750 words. Retrieved from https://studentshare.org/biology/1468260-pv92-practical
(PV92 Practical Assignment Example | Topics and Well Written Essays - 1750 Words)
PV92 Practical Assignment Example | Topics and Well Written Essays - 1750 Words. https://studentshare.org/biology/1468260-pv92-practical.
“PV92 Practical Assignment Example | Topics and Well Written Essays - 1750 Words”, n.d. https://studentshare.org/biology/1468260-pv92-practical.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document

CHECK THESE SAMPLES OF PV92 practical

Lab Report Practical Fluorescence Microscopy Practical

...?Laboratory report on Practical Fluorescence Microscopy Practical a & b Use and imaging of cultured animal cells Submitted By Bearing student no Your student number On Date For the Course (CELB40100) Summary: A laboratory work was done on fluorescence microscopy with an aim to get practical knowledge on the use and imaging of cultured animal cells. For this study, pre-cultured HeLa cells were counted and then plated on coverslips placed in culture dishes. The cells, attached to the coverslips, were washed with phosphate buffered saline (PBS) and fixed using chilled methanol or paraformaldehyde (PFA), depending upon the protein to be stained. The PFA fixed cells were permeabilised using...
7 Pages(1750 words)Essay

Practical

...? Practical Report on Children and Young People’s Self Identity Children and young people exhibit differences in the way they behave and how they relate with other people, which constitute their personality and individual character traits. This largely involves how children develop a sense of identity in the way they see themselves and how other people view them. This sense of identity develops and changes through a period of time along their childhood. Every person has a unique gradual development of a sense of autonomous and individuality which is relatively different from other people. This concerns the child’s social behavior and his own view about how people relate with him or her depending on the expectations of...
7 Pages(1750 words)Essay

Practical report

...hybrids with complementary single strand template DNA strands resulting into short double-strand regions that extend by a DNA polymerase in 5’ to 3’ direction. This results in an exponential buildup of the definite target section, approximately 2n where n is the amount of cycles of magnification performed. Figure 2 below shows a pictorial expression of PCR. Fig 2 3.0 Aim and Hypothesis The aim of this project was to investigate purification of genomic DNA from buccal cells by use of Agarose Gel Electrophoresis. Practical 1: Purification of Genomic DNA from Buccal Cells 4.1 Methods and Materials 4.1.1 Materials 10 ml of 0.9% saline, sterile 50 ml Falcon Tube, centrifuge, stop watch, pipette, Chelex beads, heater (Bunsen...
8 Pages(2000 words)Essay

Practical

...? Practical: Examining 6 to 12 Year Old Children’s Understanding of Why Some Objects Float and Others Sink This study tried to explore how children of different ages explain why some objects float while others sink. Data was collected from two female participants between the ages of 6 - 12 years who studied in the same school via a video-recorded session with a researcher. Each child was shown a number of items and asked to predict whether it would float or sink and to provide explanations. They were also challenged on inconsistencies. It was found that children did indeed try to develop a consistent theory for why some objects float and others sink, and that maturity was associated with more complex explanations. The involvement... Practical:...
10 Pages(2500 words)Essay

DNA Practical

...Aim To isolate the DNA from the tissues of kiwi fruit and to study how to visualize DNA using gel electrophoresis. Introduction DNA is the genetic material responsible for the development of all forms of life. It is found in the nucleus, mitochondria and chloroplast (in plant cells). In nucleus, DNA is associated with the histone proteins and is linear in shape whereas in organelles like mitochondria and chloroplasts, DNA is circular and is not associated with histone protein. The DNA from mitochondria and chloroplast is responsible for cytoplasmic inheritance or maternal inheritance. DNA is a double stranded nucleic acid and both the strands run antiparallel. It is made up of pentose sugar (de-oxy ribose), phosphate group... To isolate the...
4 Pages(1000 words)Lab Report

Practical 5

...Practical 5 Practical Report Two Question According to Weaving (2007, p.27), the graph for intravenous injection of antibiotic will the highest from the start, until all the drug content has been thoroughly utilized because, the drug will be in direct contact with blood, with no restricting membranes such as the stomach linings. Intramuscular injection will have a high graph but slightly lower than the intravenous injection. Oral injection will have the lowest level and, hence the lowest graph due to the many lining which restrict direct entry of drug into the blood stream. Lining in the stomach and the dilution of drug content by the saliva and enzymes found in the saliva such as amylase will also lower the content. Question 2... infection...
2 Pages(500 words)Coursework

Pharmacology Practical

...PANCREATIC ASSAY INHIBITION ASSAY ID Pharmacology Practical 09 December Pancreatic Lipase Inhibition Assay Results Table 1: Absorbance values and final Orlistat concentration after incubation Tube no. Final conc. Orlistat (µg/mL) Absorbance at 410 nm (A1) Absorbance at 500 nm (A2) Absorbance A1 – A2 = A3 1 100 0 0 0 2 80 0.098 0.010 0.088 3 60 0.078 0.008 0.007 4 40 0.121 0.061 0.006 5 20 0.104 0.031 0.073 6 10 0.123 0.019 0.104 7 5 0.190 0.110 0.08 8 1 0.123 0.040 0.083 9 0.5 0.156 0.060 0.096 10 0 0.361 0.188 0.173 Figure 1: Plot of absorbance values and final Orlistat concentration after incubation Estimating IC50 value One of the data points in the series was clicked to add a trendline...
2 Pages(500 words)Essay

Practical 1

... Annealing This process was performed on test pieces of copper at the temperature of 500ºC. The results obtained from the experiment give an approximated behavioral pattern of the various cold worked samples of copper. During the process, it was observed that the specimen have increasing roughened surfaces as the degree of rolling is increased. This is due to dislocation pile up at the grain boundaries. The hardness of the sample during cold rolling increases with increase in reduction in the cross-sectional area or degree of deformation. This is attributed to strain hardening. The maximum value of hardness was obtained at 70% reduction in area. (a) What property must be exceeded for rolling to successfully change a materials... Annealing...
1 Pages(250 words)Assignment

Practical Life

...Montessori: Practical life Insert Insert Montessori became one of the most influential shapers of the school systems. She came up with innovative classroom practices that have since shaped the education of young children. She graduated as a medical doctor from Rome medical school. Her passion in education drove her to help young children with special needs. Through her practices, she concluded that children learn best by doing. In addition, happy self-motivations assisted the children develop better. The discovery led her to write articles and journey across the world establishing schools to serve children. For example, coming up with a Montessori classroom and environment oriented to...
4 Pages(1000 words)Research Paper

Practical

................................................................................................................................................10 Task 3.............................................................................................................................................11 Syntax used to create Data.............................................................................................................12 References......................................................................................................................................14 Introduction The project is the practical steps that lead to the design and implementation of an oracle database for a company called The Concert...
10 Pages(2500 words)Assignment
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Assignment on topic PV92 practical for FREE!

Contact Us