quora

Microbial physiology and culture (Practical 1 Antibiotics and Agar Diffusion) - Coursework Example

Comments (1) Cite this document
Summary
Practical 1: Antibiotics and Agar Diffusion Insert name (s) Course Abstract Agar plate diffusion methods are increasingly being used in the study of the antibiotic effects on various microorganisms. The methods primarily involve the quantification of the ability of a particular antibiotic to inhibit bacterial growth…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER92.2% of users find it useful
Microbial physiology and culture (Practical 1 Antibiotics and Agar Diffusion)
Read TextPreview

Extract of sample
"Microbial physiology and culture (Practical 1 Antibiotics and Agar Diffusion)"

Download file to see previous pages On the other hand, experiment 2 attempts to investigate the repeatability of the essays using two different methods of antibiotic application to the discs. Lastly, experiment 3 uses gradient plates to qualitatively compare the response of new isolates to a particular antibiotic against a response of a standard strain. Practical 1: Antibiotics and Agar Diffusion Introduction Agar plate diffusion is currently one of the widely used experimental methods to determine the susceptibility of various microorganisms to antimicrobial agents. Generally the principle employed in this method is dependent on the inhibition of the microorganism reproduction on the surface of agar medium by an antimicrobial agent which gradually diffuses into the medium through a filter paper disc. In this regard, the level of susceptibility of the organisms is indicated by the size of the zone of inhibition. Typically when an antibiotic is applied to the agar medium, it moves from its region of high concentration to the surrounding areas of lower antimicrobial concentration (Cooper, 1993, p.24). Inhibition zone is the clear area of the agar plate where the growth of the microorganisms is prevented by the activities of the microbial agent. The size of inhibition is usually measured and then compared to the standardized measurements in order to determine the level of antibiotic activity on the particular medium. This is primarily because the diameter of the inhibition zone is always a function of the susceptibility of the microorganism and the amount of antibiotics on the medium. Generally there are a number of factors that may affect the antimicrobial activity and consequently determine the size of inhibition zones. Some of the likely factors include agar depth, size of the inoculums and the presence of cations on the medium. The objective of this experiment is to investigate the factors that are responsible for the variation of microbial activity of various antimicrobial drugs on different organisms. Methods To investigate the effects of variables such as agar depth, presences of salt and inoculums size on the size of inhibition zones, lawn and seeding methods were used to inoculate the plates. In determining the effect of agar depth on the result of agar plate diffusion, four plates containing 10 cm3, 20 cm3, 20 cm3 and 30 cm3 agar were first swabbed with Eschericia coli 10418 (1:20 dilution). All plates were oriented horizontally except for the third one, which was slanted. Meanwhile, 2 plates each containing 20 cm3 DSTA were inoculated with 100 µl of either 1:20 E.coli dilution or undiluted broth culture. 4 discs each containing 20 µl 0.5 mg/ml ampicillin were placed in each of the plates. Finally the plates were then incubated overnight at 37°C and the variations in the inhibition zone size were recorded. To determine the effect of inoculum size on the antibiotic activity, 0.5mg/ml ampillicin solution and the E.coli dilution were used.2 plates with 20 cm3 DSTA were poured into the solution. The first inoculate was then spread uniformly on the entire surface of the plate. On the second plate was also inoculated with a containing broth culture of E.coli was also inoculated. Finally 4 amplicin impregnated papers were placed on each plate before they were incubated. To investigate the effects of potassium or calcium ions on zone sizes, 0.1 or 1.0 ml of 2M KCl or CaCl2 ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Microbial physiology and culture (Practical 1 Antibiotics and Agar Coursework”, n.d.)
Retrieved from https://studentshare.org/biology/1460543-microbial-physiology-and-culture-practical
(Microbial Physiology and Culture (Practical 1 Antibiotics and Agar Coursework)
https://studentshare.org/biology/1460543-microbial-physiology-and-culture-practical.
“Microbial Physiology and Culture (Practical 1 Antibiotics and Agar Coursework”, n.d. https://studentshare.org/biology/1460543-microbial-physiology-and-culture-practical.
  • Cited: 0 times
Comments (1)
Click to create a comment or rate a document
pr
princess70 added comment 1 month ago
Student rated this paper as
I never thought 1500 words essay could be written in such a free manner. I loved the research of this essay. Will definitely use it for my own work!
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Human and Plant Physiology
Leaves are thin, and this makes the diffusion of gases (oxygen and carbon dioxide) during gaseous exchange short. The midrib and the wide network of veins offer good support for the blade or the thin lamina. The broad network of veins facilitates efficient transportation of materials to and from the photosynthesizing cells.
4 Pages(1000 words)Coursework
Culture intelligence
It is very important to understand how business communication is impacted by culture and how employees and businesses interact globally. This can be done only by training workforce on Cultural Intelligence. Teams and organisations that are at odds with one another and co-operate well across the cultures will prosper in an increasingly global economy.
1 Pages(250 words)Coursework
Effects of Drugs Misuse on the Human Anatomy and Physiology
It is therefore paramount the body is treated well and all body systems kept in good working condition just as any other system in order to function normally. Substance abuse is one of the many factors or issues that would alter the normal functioning of the body.
6 Pages(1500 words)Coursework
Organisation Culture
Grameen Bank is an organization focusing on the support of people of low income; more specifically, the bank aims to help people of low income to enter the business field. The Bank, which was
14 Pages(3500 words)Coursework
Practical 5
Intramuscular injection will have a high graph but slightly lower than the intravenous injection. Oral injection will have the lowest level and, hence the lowest graph due to the many lining which restrict direct entry of drug into the blood stream. Lining in the
2 Pages(500 words)Coursework
Chapter 1 disscussion 1
In the United States, Chinese food has been Americanised in order to appear as if it belongs to Americans. This assimilation has led to a lot of American people accepting Chinese food as their own rather than being Chinese. Chinese food is taken to be family
1 Pages(250 words)Coursework
Indigenous Culture
The Naga people inhabit North Western Burma and North East India. The people speak a number of languages including Lotha, Sumi, Ao, Pochuri, and Mao among several others. The languages
2 Pages(500 words)Coursework
Culture outline
It is difficult to meet he values of a given culture. Symbols and language – Members of a given community may speak a given language or use common symbols. Symbols such as gestures and body movements help members of a community to understand what goes on in the
2 Pages(500 words)Coursework
Practical report
Timing was considered to be very critical in the overall experiment. Basing on the results, it is clearly evident that heart rate and Vo2 increased with increase in work load or exercise. The main objective of the practical was to assess and monitor the heart
4 Pages(1000 words)Coursework
Energy in food lab practical
The high standard deviation of the results could be due to the size of the samples and also errors in measurement of the heats of combustion arising from the arrangement of instruments or human
4 Pages(1000 words)Coursework
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Coursework on topic Microbial physiology and culture (Practical 1 Antibiotics and Agar Diffusion) for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us