Cell Adhesion Practical - Term Paper Example

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The author describes the assay which relies on the interesting effects that trypsin has on these cadherin molecules. In the presence of calcium and trypsin, the cadherins are protected from cleavage, but in the presence of a trypsin and EDTA mixture, the cadherins are removed from the cell surface. …
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Cell Adhesion Practical
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Download file to see previous pages Cell adhesion assays can be used to explore cell-matrix interactions in several different types of cell, including epithelial and tumor cells, and thus assays are very important in all areas of biology and biomedical research. One of the most important areas of recent research using assays has been the investigation of cadherins, which are cell-cell adhesion molecules, dependent on Ca2+, which play an important role in ‘tissue construction and morphogenesis’ (Asano et al, 2004). Cadherins are found as trans-membrane proteins (Rastogi, 2007). There are four main types of classical cadherins, with the epithelial cadherin playing an important role in the growth of tumors (Asano et al, 2004). Cells possessing cadherins generally adhere to other cells possessing cadherins. By exploring these cell adhesion mechanisms with an assay, it is possible to understand more fully the role of the intracellular matrix and the reactions which occur within it. Results Trypsin is commonly used to detach the adhering cells (Humphries, 2001). Prior to the addition of trypsin, the cells were adhering to each other forming a suspended clump of cells within the wells. However, after the trypsin was added, there was less evidence of adhesion and the cells were in the matrix singularly. After fixing, only the adhering cells were found in the well. This again gave a ‘clumped’ look to the cells. ECM Component Preference As was previously discussed, cadherins are of various different types and thus may have different preferences for the extracellular matrix in which they react. In the data above, the cells A1-4 represent matrix 1, A5-8 represent matrix 2, and A9-12 represent matrix 3. The cells B1-4 represent the BSA and B5-8 and B9-12 represent tube 1 and 2 respectively. In this case, the data obtained experimentally shows that there is a definite component preference for matrices 2 and 3, with the first one having a very low absorbance value and thus a very low amount of adhering cells.  ...Download file to see next pagesRead More
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