Nobody downloaded yet

The Mechanism of Dis2 Phosphorylation by Chk1 and Cell Cycle Regulation - Dissertation Example

Comments (0) Cite this document
Study on the Mechanism of Dis2 Phosphorylation by Chk1 and Cell Cycle Regulation Contents PAGE Declaration:…………………………………………………………………………i Acknowledgment v Hypothesis vii 1. Abstract ix 2. Abbreviations: xii 3…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER92.6% of users find it useful
The Mechanism of Dis2 Phosphorylation by Chk1 and Cell Cycle Regulation
Read TextPreview

Extract of sample
"The Mechanism of Dis2 Phosphorylation by Chk1 and Cell Cycle Regulation"

Download file to see previous pages PP1 and its role as a mitotic checkpoint xxxii 1.6. PP1 and cell cycle control xxxiv 1.7. Importance of regulatory subunits and their role in diseases xxxv 1.8. Human paralogues of Dis2 xli 1.9. Conclusion xliv Chapter 2 xlvii Materials and Methods xlvii 2.1. Preparation of media xlvii 2.2. Preparation of buffers xlviii 2.3. Preparation of stain l 2.4. Preparation of normal SDS-PAGE buffer and gels l 2.5. Preparation of PEMS solutions liii 2.6. Preparation of protein extracts for use in SDS-PAGE liv 2.7. Running of SDS-PAGE: lvii 2.8. Construction of yeast strains lviii 2.9. Preparation of membrane lx 2.10. Chk1-HA shift experiment lx 2.11. TCA protein extraction lxii 2.12. Immune localization of proteins in yeast cells lxiv 2.13. Drop test lxviii 2.14. Preparation of cells for imaging lxix 2.15. Acute cell survival lxxi Chapter 3 lxxiv Results lxxiv 3.1. Dephosphorylation of Chk1 at 40°C is not affected by Dis2 phosphatase lxxiv 3.2. Dephosphorylation of Hus1 at 40° lxxviii 3.3. Hypersensitivity to DNA damaging agent lxxxi 3.4. Structural changes to cells lxxxiv 3.5. Study on cell survival lxxxvi 3.6. Identification of hus1 isoforms xcii 3.7. Comparison of Dis2 with other proteins xciv Chapter 4 xcix Discussion xcix 4.1. Conclusion civ 5. Appendix cvi 5.1. Appendix – 1 Multiple sequence alignment of dis2 protein cvi 5.2. Appendix – 2 Alignment of dis2 from S. pombe with human protein serine/threonine phosphatase cviii 5.3. Appendix - 3 Significance sequence alignment of protein serine/threonine phosphatase-1 cxi Acknowledgment This thesis was made possible by the unrelenting support of my supervisors and peers. I thank the university and the department for providing me with the technical as well as educational support apart from laboratory facilities for carrying out this research. It has been a great pleasure to complete this thesis under the support and guidance of my professors. Hypothesis Chk1 kinase is phosphorylated at serine 345 in response to DNA damage. Dis2 dephosphorylated this residue slowly when cells recover from a DNA damage-induced cell cycle arrest. A rise in temperature from 30°C to 40°C results in the rapid dephosphorylation of S345 by a yet unknown phosphatase. The purpose of this experiment is to investigate the requirement of Dis2 for the heat-induced phosphorylation and to investigate the cell cycle roles of this enzyme. Other phosphor-proteins such as Hus1 and Rad9 are also investigated. 1. Abstract Protein phosphatases are a group of enzymes which have very specific role in biological cell activities. Dis2 is a PP1 enzyme (serine-threonine phosphatase-1) which plays a key role in regulation of DNA damage signaling. Fission yeast Dis2 regulates the DNA damage respons by dephosphorylation of chk1 kinase at Ser 345. In eukaryotic cells, phosphorylation mainly occurs on three hydroxyl-containing amino acids, namely – serine, threonine, and tyrosine, of which serine is the predominant target. Dis2 dephosphorylates the DNA damage checkpoint kinase Chk1 (at Ser-345) to switch off the checkpoint signal. Interestingly, heat stress results in very rapid removal of the phosphate from Ser 345 by a yet unknown phosphatase. Given the requirement of Dis2 for the dephosphorylation of Ser 345 at the normal growth temperature of 30°C, this study was conducted to investigate the role of this phosphatase under heat stress condition modification of Ser 345 is easily detected as a band shift of the protein which changes from a closed, low activity conformation ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“The Mechanism of Dis2 Phosphorylation by Chk1 and Cell Cycle Dissertation”, n.d.)
The Mechanism of Dis2 Phosphorylation by Chk1 and Cell Cycle Dissertation. Retrieved from https://studentshare.org/biology/1401154-the-role-of-the-phosphatase
(The Mechanism of Dis2 Phosphorylation by Chk1 and Cell Cycle Dissertation)
The Mechanism of Dis2 Phosphorylation by Chk1 and Cell Cycle Dissertation. https://studentshare.org/biology/1401154-the-role-of-the-phosphatase.
“The Mechanism of Dis2 Phosphorylation by Chk1 and Cell Cycle Dissertation”, n.d. https://studentshare.org/biology/1401154-the-role-of-the-phosphatase.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
Does Audit Regulation Ensure Auditor Independence
This research paper shows that independence has been examined as "an intensely felt professional philosophy". It is supposed that part of the solution in deciphering ethical impasses regarding potential and identified risks to assessor independence is to put more stress on professional beliefs.
41 Pages(10250 words)Dissertation
Analysis of Small Molecule Analogues of the helminth immunomodulator ES-62 for inhibitory effects on mast cell function
) 4, Discussion 5, Bibliography Acknowledgement Abstract Atopic allergy occurs due to an increase in IgE antibodies that elicits an immune response by binding to the high affinity receptor, Fc?RI, found on the surface of mast cells. In the Western world where parasitic infections are rare, type 2 immunity frequently causes major inflammatory diseases and allergies.
4 Pages(1000 words)Dissertation
Table of Content List of Abbreviations: 3 List of Abbreviations 3 Acknowledgement 4 Abstract 5 1.0 Introduction 6 2 Materials and Methods 14 2.1 Laser Scanning Confocal Microscopy 14 2.2 Investigation of Madin-Darby Canine Kidney (MDCK) cells in infection assay with GFP-tagged Cj 15
29 Pages(7250 words)Dissertation
Mechanism Of Trade Dispute Settlement In The Light Of International Trading System
With the onset of globalization, the world has steadily drifted towards an integrated international market. The result is an unprecedented increase in international trade. For some economists this development is both crucial and imperative in the universal pursuit for prosperity and the age-old quest for human development.
3 Pages(750 words)Dissertation
Mutual Fund Regulation
The Appellant Yu on behalf of himself and others, appeals the judgment given by the United States District Court Southern District of New York on 31st March 2011 in favor of the Respondent State Street Corporation. On appeal, the Appellant argues that the court erred in denying the Appellant’s claims against the Respondent pursuant to Sections 11 and 12 of the Securities Act of 1933 in that the Appellants had failed to prove loss causation within the meaning of Section 10(b) of the Securities Act 1933.
25 Pages(6250 words)Dissertation
The Regulation of International Capital Doctrine
In recent years there have been thousands of bilateral investment treaties (BITs). A parallel development is the perception that BITs have or will create a special limb of international customary law. It has been argued for the most part that BITs have evolved to such an extent that they have their own unique regulatory laws.
5 Pages(1250 words)Dissertation
The Cell Cycle
Mitosis, on the other hand, involves division of nuclear chromosomes. It has four phases namely the prophase, metaphase, anaphase, and telophase. In prophase, chromatin material is condensed into distinct chromosomes that migrate to the center of the cell. At the opposite poles of the cell, there is the formation of spindle fibers.
2 Pages(500 words)Essay
A study into the effectiveness of existing cycle lane: Effect of cycle lanes at priority junction
Any section, part or phrasing of more than twenty consecutive words that is copied from any other work or publication has been clearly referenced at the point of use and also fully described in the reference section of this dissertation.” Signed……………………………………………………… Acknowledgments During the period of this research many people have helped by being next to me.
5 Pages(1250 words)Dissertation
Is the EU merger control regulation necessary Is it a good system
This paper shall present the advantages and infirmities of the law and based on this dissection, this writer will make a conclusion on whether the Merger Law is good as it is or is it prejudicial warranting amendments to achieve the goals it set out at its inception.
16 Pages(4000 words)Dissertation
Embryonic stem cell
The proposed qualitative case study has the purpose to explore bioethical factors and political/social controversy on stem cell research with the aim to identify and explore the potential impact stem cell research has on behavior and attitudes of health care professionals with the focus on three medical facilities.
14 Pages(3500 words)Dissertation
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Dissertation on topic The Mechanism of Dis2 Phosphorylation by Chk1 and Cell Cycle Regulation for FREE!
logo footer
Contact us:
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us