Factors to Be Considered in Conducting Plasmid Purification - Term Paper Example

The paper "Factors to Be Considered in Conducting Plasmid Purification" is an excellent example of a term paper on biology. There are various ways through which DNA purification can be done and plasmid purification happens to be an example of these. In most cases what happens when conducting plasmid purification is that there are some kits involved in the process.

These kits are what will help an individual to isolate the plasmid DNA on the basis of the scale and the purity that is needed. (Horak, 1998) In this regard, there are some things that are to be put into consideration when conducting plasmid purification. In this case, it will be of the essence to consider some of the things that are of importance when carrying out this process. Possession of a plasmid number This determines the volume of culture that will be used in the purification process.

Again in this same regard, it should be noted that manipulation of some of the properties of these plasmid patterns may influence the final outcome. Such properties include the size, origin of replication among other things. These will influence the outcome in terms of the quality and the DNA yield. The number attached to this will influence the binding capacity, for example, QUIGEN-tip 100 and QUIGEN-tip 500. The nature of the host bacteria The strain of the bacteria used will go a long way in influencing the quality and the quantity.

It has been seen that when high amounts of carbohydrates have released the quality of the DNA that is released will be comparatively lower. At the same time, high endonuclease levels during lysis will bring about low DNA quality. These are exhibited in HB101 and the JM100 series among others. The Background of the chosen bacteria There should be ambiotic selection in all aspects of growth. This ensures that the cells not receiving plasmid fails to continue to grow and thrive even under the pressures of ambiotic.

The sensitivity of these ambiotics to temperature changes is also another fact that should be taken into account. Application of Plasmid Purification Gene Therapy There are some gene therapies whose success is greatly dependent on the insertion of some genes which prove to be therapeutic and in this case without causing mutations, injury or an immune response.