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(54), based on the results shown in a and b figures, Gα12/13-induced MMP-2 upregulation, invasion/migration needs Ras- and Rac. How were the researchers able to arrive at such conclusion? First, in figure a, it is shown that Gα12/13QL activation is…
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Download file to see previous pages Possibly, the decrease in MMP-2 in the presence of interfering mutated Ras resulted to the observed decrease in the amount of invaded cells.
B) Based on figure b, MMP-2 signal is very apparent when the mutated Ras, N17Ras, is absent. On the other hand, MMP-2 signal is decreased in the presence of N17Ras. And since in the next figure the absence of N17Ras was shown to increase the number of invaded cells per field, then it is highly likely that invasion can be attributed to the presence of MMP-2, which is Ras-dependent. Again, as mentioned in part A, N17Ras competes with the wild-type Ras in the latter’s interaction with the factors that lead to the upregulation of MMP-2 (Kin et al. 55)
These findings were determined using gelatin zymogram assay, as well as in vitro invasion and transwell migration assays. Particularly, gelatin zymogram assay determines the presence or absence of gelatinolytic activity of the conditioned media. This assay takes advantage of the inherent gelatinolytic activity of MMP-2 to determine the molecules presence or absence. Thus, liquidation of gelatin predicts the presence of MMP-2 (Kin et al. ...Download file to see next pagesRead More
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