Advantages and disadvantages of western blot and DNA/RNA microarray - Essay Example

The use of microarray technology involves the construction of microarrays in four steps.... The use of microarray technology to study gene-expressions in yeast S....  In line with this step, a microarray-referencing sample should be chosen in order to ensure that there is a collection of the adequate sample during the experimental procedure.... Following the selection of the sample, the next step involves the isolation of rna and probe generation....

The varying magnitudes of methylation in the cells are associated with different stages of cancer development, and dna methylation technique can be used to determine the exact stage (Costello et al, 2000).... Scientists and researchers point out that gene expression anomalies mostly involve the Messenger rna (mRNA).... This limitation necessitated the introduction of rna sequencing in which unknown genes expression can be studied (Cloonan et al, 2008)....

For instance, it has been utilized for multiple purposes – to quantify mRNA levels (RT-PCR); produce DNA fragments for the purposes of cloning and subcloning; label and identify… 2005). Through PCR, the detection of RNA and dna has fully evolved.... In summary, the following are the marked disadvantages on the use of traditional PCR: poor precision, low sensitivity, short dynamic range ...

Domestic dogs are considered the perfect models for the study.... This is because dogs form spontaneous tumors and at the same time they have a tendency towards… Further the field of animal veterinary and the discipline of human medicine applies the same tools for diagnosis and therapy.... Additionally, the response of the canines to chemotherapies has more similarity to that of humans, when Therefore, dogs become the best models for indentifying the genetic underpinnings that are associated with cancer in humans (Mooney, et al....

In western blot the method involves the separation of the proteins of an individual in accordance to the sizes through the use of a polyacrylamide gel (Wikipedia, 2013).... DNA and rna microarrays have been used in the determination of the infection with HIV since they are the key ways in the understanding of the expression of the genes and the method also allow for the rapid quantification of many of the genes once in the given cell that is focused....

This results to latent infection in cellular reservoirs (asymptomatic) (Kassutto & Rosenberg HIV DETECTION BY western blot and dna/RNA MICROARRAY due: Introduction The HIV virus The human immunodeficiency virus type 1 abbreviated as HIV-1 is a retrovirus, which is the cause of AIDS.... NA/RNA MicroarraysThis technique uses DNA/rna microarray chips to detect gene expression of thousands of specific genes (Sealfon & Chu 2011, p.... RNA and dna microarrays....

The paper discusses the main advantages and disadvantages of the methods used, including conclusions.... The paper "HIV-1 Detection by western blot" discusses the experiment, in which the two microarrays are used where two samples are prepared and the results that are obtained and compared....

In microarray data testing two approaches are used in the analysis for different types of genes.... microarray data quality control is a critical area whereby the problems of implication, outlier, and unnecessary conditions need to be understood and eliminated.... ackgroundAcademic literature teems with articles discussing microarray data quality control as it relates to gene expression differences among patients in control and treatment groups of drug testing....