StudentShare
Contact Us
Sign In / Sign Up for FREE
Search
Go to advanced search...

Screening a genome wide deletion library for mutants sensitive to gemcitabine - Essay Example

Cite this document
Summary
Gene mutant: Name: Institution: DNA Repair Genes The human genome is made up of the genetic information in individual DNAs. DNAs characterize the proteins formed. Damage to the DNAs might arise from exposure to radiations which ionize or even from ultraviolet rays…
Download full paper File format: .doc, available for editing
GRAB THE BEST PAPER95.4% of users find it useful
Screening a genome wide deletion library for mutants sensitive to gemcitabine
Read Text Preview

Extract of sample "Screening a genome wide deletion library for mutants sensitive to gemcitabine"

Download file to see previous pages

The other type of repair is nucleotide excision. In the repair, the genes are linked to DNA glycosylases (Wei & Chen, 2006). Some of these genes are listed thus: UNG OGG1 MPG NEIL MGMT (Wei & Chen, 2006). The above image shows highlighted DNA genes Checkpoint Genes Damage can occur on the DNA during cell division causing it to cease dividing. This is what is referred to as checkpoint damage. Causes of the damage might be due to radiations such as from ultra violet rays. Checkpoint genes help in repair of DNA at the points of breaks through a response in the body.

In the case of humans, these genes are defined as the G2 checkpoint genes. They include RAD17 as well as RAD1. The other genes include HUS1 (Stern, 2002). Rad13 Rad13 is a human gene. Its product is involved in DNA repair and it gives rise to a nuclease which is christened Rad13 nuclease. The gene is about 80mm. the systematic identification of the gene is SPBC3E7.08c. Its characterization is also known. The following is an image of the gene (Caspari, 1985). The modern society has been taken through environmental changes that necessitate the adoption of genetic science.

The results in this experiment shows the findings from a genetic mutation test carried out in the laboratory. What needs to be understood is the process that facilitates this type of reaction. Some factors need to remain constant during the laboratory experiment so that there is high accuracy and efficiency in the findings. It should be noted that the required temperature for tests and subsequent analysis and interpretation should remain around 250c – 30oc for the specified time period. The two strains under question must also be cultured on a solid medium which will provide the best medium for a desirable outcome.

In this test, the point of focus is the strain that grows on the EMM plus NAT and EMM plus Hygromycin, but not on the EMM plus CYH.This project touches partially on pharmacogenetics which majorly undertakes the studies on the role of inheritance in the variation in phenotypic response to drug. Such phenotypes ranges from serious inadequacy of therapeutic efficacy at one end to life-threatening unfavourable reactions of drugs the other end (PFEIFER, 2006). This test would be very applicable in genome-wide techniques in the clinical pharmacogenomic and its model systems which vary from yeast gene deletion libraries to cell-line based model.

The validation of the candidate genes plus the application of genome-wide technology is essential for following up the identification of the candidate genes. In the strategy for genetic model specification during the screening of the geno-wide, an easy-to-use Bonferroni-corrected method which is multipurpose in the sense that it fits both recessive and multiplicative model is found to be reliable if used. In the context of this experiment, it is better to have in-depth understanding of what the classification of mutation is all about (SPENCER, 1997).

On the basis of effects on structure, deletion is one of the most explored in this project. In so doing; these mutations change the gene reading frame just like in the case of insertion. It is however imperative that people understand the unfitting opposite aspect of the two. Deletion is fairly random while insertion constitutes a given sequence that doesn’t necessarily take a random order. The results obtained from the experiments and tabulated were formatted in four distinctive columns for easy analysis.

In this table there are theoretical short explanations and

...Download file to see next pages Read More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Screening a genome wide deletion library for mutants sensitive to Essay”, n.d.)
Screening a genome wide deletion library for mutants sensitive to Essay. Retrieved from https://studentshare.org/biology/1458457-screening-a-genome-wide-deletion-library-for
(Screening a Genome Wide Deletion Library for Mutants Sensitive to Essay)
Screening a Genome Wide Deletion Library for Mutants Sensitive to Essay. https://studentshare.org/biology/1458457-screening-a-genome-wide-deletion-library-for.
“Screening a Genome Wide Deletion Library for Mutants Sensitive to Essay”, n.d. https://studentshare.org/biology/1458457-screening-a-genome-wide-deletion-library-for.
  • Cited: 0 times

CHECK THESE SAMPLES OF Screening a genome wide deletion library for mutants sensitive to gemcitabine

Carcinogenesis and Cancer Treatment Resistance

MMR is the pathway repairing deletion, mismatch or insertion mutations that happen in the DNA and TLS pathways makes the polymerases bypass modified bases which can disrupt the replication forks.... 1.... Carcinogenesis and Cancer Treatment Resistance Cellular DNA was considered to be an inert substance....
19 Pages (4750 words) Assignment

Genome-Wide Screen for Salmonella Genes

The pathogenic Salmonella species are a group of intracellular gram-negative bacteria that are known to be the cause of a wide range of diseases across the globe.... The subspecies enterica in particular is alleged to cause about 99 percent of these pathological conditions.... hellip; Figures from the World Health Organisation report of 1997 suggested that it is a species responsible for over 600, 000 deaths per annum (WHO 1997)....
9 Pages (2250 words) Essay

GFP Mutants with Altered Mass Spectra

The aims of this study “GFP mutants with Altered Mass Spectra” are to introduce mutations by site-directed mutagenesis to recombinant GFPuv DNA sequence, express the mutated protein and compare the mass spectrometric properties and fluorescence intensity of the mutant GFPuv with the wild type GFPuv....
9 Pages (2250 words) Research Proposal

DNA repair mechanisms role in survival to nucleoside analogues treatment in S.pombe

Edgar Hartsuiker for his support and Andrea for her invaluable advice and supervision.... I am grateful to the Government of the Kingdom of Saudi Arabia for support me to finished this course. The human is exposed to a large number of agents each day that… Repair mechanisms play a crucial role in correcting the damage that has occurred, and allowing the cell to continue to replicate....
28 Pages (7000 words) Thesis

Difference between PM19-Containing and PM19-Absent Cell Lines

In this… After 3 and 6 days after culture, it was found that trk1 mutants had poorer growth compared to the WT and tok1 mutants.... Because of its structure, its role in transcellular transport is likely.... S.... cerevisiae cell lines with mutated K+ channels is a good study model to determine whether PM19 has a role in K+ transport or not....
19 Pages (4750 words) Essay

Discuss the use of directed evolution in enzyme technology

As highlighted above, the process of rational design that comprised of screening produced DNA libraries and subsequently engineering them did not address most of these setbacks (Arnold & Georgiou 2003, p.... Nature designed enzymes as molecules that ensure that processes occur at the appropriate speeds....
8 Pages (2000 words) Essay

Applications in Gene Selection and Cloning

This report "Applications in Gene Selection and Cloning" focuses on the basic process of inserting known gene sequences into vectors and cloning these using a variety of host cells has many applications that have a wide range of uses, from medicine to agriculture.... The basic process of inserting known gene sequences into vectors and cloning these using a variety of host cells has many applications that have a wide range of uses, from medicine to agriculture and even in the identification of relationships among organisms....
11 Pages (2750 words) Report

The Effect of Amyloid Prions and Second-Site Suppressor Mutations

The first notable phenotypic character trait is that vpr 1 mutant cells are temperature sensitive.... According to research, temperature-sensitive mutant genes are observable in different cultures.... In this study, the AMY89 vrp1Δ mutants #1 showed significant growth at 24oC.... This exemplifies the fact that the mutants behave differently when exposed to various temperature conditions....
7 Pages (1750 words) Term Paper
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Contact Us