StudentShare
Contact Us
Sign In / Sign Up for FREE
Search
Go to advanced search...

Rate of Glucose Uptake by Yeast Cells - Lab Report Example

Cite this document
Summary
When yeast cells are added to a glucose solution, the glucose molecules are absorbed across the cell membranes. As time progresses, the concentration of glucose falls. When the surrounding temperature of the yeast cells is increased drastically, the yeast cells die and further uptake of glucose is stopped…
Download full paper File format: .doc, available for editing
GRAB THE BEST PAPER92.4% of users find it useful
Rate of Glucose Uptake by Yeast Cells
Read Text Preview

Extract of sample "Rate of Glucose Uptake by Yeast Cells"

Download file to see previous pages

From the equation, it can be concluded that one mole of glucose reacts with two moles Cu2+ to give one mole of Cu2O. Thus, one can weigh the mass of Cu2O formed and relate it to the amount of glucose present in a solution. This method can be used to study the rate of glucose uptake by yeast cells. 2. Start the timer and add 1.00 mL of yeast suspension with a micropipette into one flask containing the glucose solution. Repeat this until 7 replicates are obtained. Please note that this has to be done very fast (within 1 minute if possible).

To the remaining flasks, add 1.00 mL of distilled water into each, and label them as blank. 4. After 30 minutes have elapsed, pour the contents of one flask labelled blank sample and one containing the yeast suspension into two separate beakers containing around 150 mL of boiling distilled water. Cool the mixtures to room temperature and transfer them into two separate 250-mL volumetric flasks. Add distilled water to the flasks to make a final volume of 250.00 mL. Shake the flasks well and let them stand until clear supernatants are obtained (the blank solution should not have a sediment if the experiment has been done properly).

Label the one obtained from the blank sample as B60 and the other one as Y60 5. Pipette 25.00 mL of supernatant from B60 and pour it in a 100 mL beaker. Add 25 mL of 0.2500 M Benedict's solution (it is in excess) to the beaker containing the supernatant from B60. Heat the resulting mixture to boiling until a red precipitate is formed. Cool this mixture to room temperature. Repeat to get a replicate.6. Filter the mixture with a Gooch crucible tared with filter paper using the set up shown in Figure 1.

Wash the precipitate several times with cold distilled water.Figure 1. Set up for filtration apparatus7. Use a cloth (this would avoid fingerprints on the surface) to remove the crucible containing the precipitate from the filtration apparatus.8. Dry the sample to constant mass. Ensure that tongs are used to handle the crucible and that the sample is kept/cooled in a dessicator before weighing.9. Repeat steps 5-8 with Y60.10. Repeat steps 4-9 to get data at t =120,180,240,300,360,420 mins and label the samples accordingly.

CalculationsAs per Equation 1, one mole of glucose reacts with two moles of Cu2+ to give one mole of Cu2O. In the above experiment, glucose is the limiting reagent and Cu2+ is in excess. Hence the amount (mole) of Cu2O formed of is directly proportional to the amount of glucose in the solution.Molar mass of Cu2O = (63.55 x 2) + 16 = 143.10Molar mass of glucose, C6H12O6 = (12.01 x 6) + (1.01 x 12) + (16.00 x 6) = 180.18No. of moles of Cu2O formed =

...Download file to see next pages Read More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Rate of Glucose Uptake by Yeast Cells Lab Report”, n.d.)
Rate of Glucose Uptake by Yeast Cells Lab Report. Retrieved from https://studentshare.org/science/1518050-rate-of-glucose-uptake-by-yeast-cells
(Rate of Glucose Uptake by Yeast Cells Lab Report)
Rate of Glucose Uptake by Yeast Cells Lab Report. https://studentshare.org/science/1518050-rate-of-glucose-uptake-by-yeast-cells.
“Rate of Glucose Uptake by Yeast Cells Lab Report”, n.d. https://studentshare.org/science/1518050-rate-of-glucose-uptake-by-yeast-cells.
  • Cited: 0 times

CHECK THESE SAMPLES OF Rate of Glucose Uptake by Yeast Cells

Role of GLUT4 Glucose Transporter

This family of glucose transporters were first discovered in yeast where 18 genes have been identified.... Activation of the insulin receptor triggers a large increase in the rate of GLUT4 vesicle exocytosis and a concomitant decrease in the rate of endocytosis.... The generalised form of this reaction is written as follows There are three types of sugar transporters that carry sugars across the plasma membrane in to the cells....
19 Pages (4750 words) Essay

Effects of Glyconutrients on the Individuals Well Being

These glycocongugates, found in surface of cells play an important role in biological activities and any imbalance of these glyconutrients would then result to disease.... N-acetylneuraminic acidBreast milkImportant for brain development and learninglowers the LDL and inhibits strains of influenza A and B, bacteria and other pathogensGlyconutrientsFood sourcesMain featureEffects of individuals well being as claimed by drug companiesXyloseGrains- wheat, rye and barleyCarrot, beet, cauliflower, broccoli, kale, lettuce, parsley, rhubarb, Brussels sprouts, red cabbage and asparagusUsed as a substitute for sucrose and corn sweetenersAntibacterial, antifungal and prevent cancerGalactoseDairy products, legumes, algae, many fruits and vegetablesFound in combination with glucose to form a disaccharide called lactoseInhibit tumour growth, enhances healing of wounds , decrease inflammation and triggers long-term memory formationGlucoseMilk, mushrooms, various fruits and vegetables and grainsUbiquitous saccharide Memory enhancement, proper calcium absorptionTable 2....
12 Pages (3000 words) Essay

Review of the N314D Point Mutation on the GALT Gene and its Relationship to Galactosemia Type I

The deficiency of this enzyme leads to the accumulation of Galactose-1-phosphate inside the cells, and that accumulation of galactose- The UDP- galactose, found in the liver and erythrocytes, is required for the major cell functions like chemical signaling, building cellular structures, transporting molecules and producing energy.... Since, galactose-1-phosphate is toxic to the parenchymal cells present prominently in liver, kidney and brain, accumulation of it in those organs leads to severe damage to them....
17 Pages (4250 words) Term Paper

Difference between PM19-Containing and PM19-Absent Cell Lines

In plants, since ABA, a hormone released in response to water desiccation, increases PM19 expression, the overproduction of the membrane protein allow the cells to imbibe the important K+ ion despite the low concentration in the environment.... The DNA sequence of an organism remains constant throughout its life despite the multitude of processes it undergoes by preventing DNA mutations that might alter genetic information, and by ensuring the structural integrity of chromosomes and their orderly transmission to progeny cells....
19 Pages (4750 words) Essay

The Effect of Barley and Oat in Blood Glucose Level in Diabetic Patients

People suffering from diabetes have a condition whereby the level of glucose in their blood is much higher than normal, scientifically known as hyperglycemia.... Typically, they had higher blood sugar levels than normal, although not high enough to warrant a diabetes diagnosis but rendering body cells resistant to insulin.... The condition is as a result of either insufficient production of insulin by the pancreas or the improper response to the insulin by the body cells, with the occurrence of both circumstances not being a rare phenomenon (Jensen et al 2004)....
10 Pages (2500 words) Assignment

Control & Co-ordination

The nervous system is subdivided into two major parts, the Central nervous system and… The peripheral nervous system greatly consists of nerve cells which are the receptors in the body.... The nervous system comprises of nerves and neurons which transmit and transfer information and signals to distinguished and different parts of the body....
7 Pages (1750 words) Assignment

Roles of Enzymes

The process of glucose breakdown is called glycolysisiii.... Stages of glycolysisStep 1 - In the first step, there is conversion of glucose to fructo-1, 6-biophosphate using ATP.... At the same time, there is trapping of glucose inside the cell which is then converted to an unstable form that can easily be cleaved to 3 – carbon units.... Six carbon of glucose enters the krebs cycle.... Organisms respire without oxygen through incomplete breakdown of glucose in the absence of Oxygen....
7 Pages (1750 words) Assignment

Homeostasis: Physiology and Pharmacology for Nursing Practice

The cells may be entirely different in terms of their functions, but similarities can be pointed out in their metabolic requirements.... A constant… ternal environment with elements such as oxygen, glucose, mineral ions, and waste removal is crucial for the cells' survival and the overall well-being of individuals (Lenford and Johnson, 2015).... An increase in blood glucose higher than the homeostatic range triggers the processes that reduce it....
9 Pages (2250 words) Essay
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Contact Us