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Light Microscopy - Essay Example

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Microscopes that use visible light as a source of illumination are called light microscopes. Today’s light microscopes are compound microscopes. They provide greater magnification and are much easier to use…
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Light Microscopy
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Light Microscopy

Download file to see previous pages... Compound microscopes have two lenses, an objective lens and an ocular lens, both of which are corrected lenses that compensate for the aberrations inherent in ordinary lenses. Uncorrected aberrations produce images that are surrounded by colored rings (chromatic aberration), and not all parts of the field of observation are simultaneously in focus.
Most modern compound microscopes have a built-in source of light. First this light passes through a series of lenses called the condenser, which concentrates the light onto the specimen, where some of it is absorbed. Then the transmitted light enters the objective lens, which forms an image of the specimen within the body tube of the microscope.
Most compound microscopes offer a choice of several objective lenses mounted on a rotating turret; each lens has a different magnifying power. High power oil-immersion lenses cover smaller fields but reveal great detail within the specimen.
Resolution is another requirement for obtaining a useful image. It is the ability to distinguish in detail within an image. For example, a television set with high resolution has a clear picture; one with poor resolution has a fuzzy picture.
Light microscopes are for everyday use. Specimen preparation and operation are relatively rapid and simple. Light microscopy provides valuable information about the size, shape and general appearance of cells. However, resolution is limited and therefore so is useful magnification. ...Download file to see next pagesRead More
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