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A comparison between two different proteomic approaches used to investigate cancer system - Essay Example

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This fatal disease is being diagnosed and treated with latest developed methods in medical sector. ‘Proteomic’ and ‘Proteome’ are keywords intensely in diagnose sector of…
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A comparison between two different proteomic approaches used to investigate cancer system
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Download file to see previous pages Also contacts and communication between the proteins that make up a specific proteome under investigation is significant in proteomics. Nowadays doctors adopt proteomic approaches in the diagnosis of cancers avoiding the pre existing diagnose techniques like Immunohistochemistry, ELISA (Enzyme-Linked ImmunoSorbant Assays), Immunoblots etc.
The proteomic approaches adopted for cancer diagnosis by medical field are mainly based on electrophoresis and spectrometry. Proteomes exhibit varied characters at various instances of simulations especially due to hormones. This is due to the variations in proteins composing those proteomes. Both approaches employed in proteomics try to dig out those characteristics making up variations in human cells. There are many approaches using both electrophoresis as well as spectrometry techniques. 2D-PAGE, MALDI-MS, SELDI-TOF-MS, Antibody arrays etc. are some specific approaches of proteomics. In this paper, the comparative proteomic approaches of 2D- PAGE and MALDI-MS in Human Lung Squamous Carcinoma and functional proteomic approach in Breast cancer.
The detailing on diagnostic functional proteomic approach used in breast cancer can be initiated with identifying basic facts on it. “Functional proteomics approaches that comprehensively evaluate the biological activities of human cDNAs may provide novel insights into disease pathogenesis.” (Witt, et al., 2005, p.1). The analysis on cDNAs is taken up through thorough investigation about their response to various stimulations especially those expected during initial stages of breast cancer. To analytically examine the practical response of cDNAs especially those involved in breast carcinogenesis, first a collection of such cDNAs are made named as Breast Cancer 1000 (BC1000). Again stimulations similar to tumour instigation and development are imposed on those cDNAs create response, but it is clear that each response corresponds to a rearrangement of proteins composing the ...Download file to see next pagesRead More
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