Nobody downloaded yet

Biology BSc third year biotech project; Manipulating Biomass and PHA Production in batch and continuous cultures of Paracoccus denitrificans - Lab Report Example

Comments (0) Cite this document
In addition if we take the square root of the regression, we find that the correlation coefficient is almost 1, indicating that as 1/S increases 1/V also increases.
From the Graph,…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER97.4% of users find it useful
Biology BSc third year biotech project; Manipulating Biomass and PHA Production in batch and continuous cultures of Paracoccus denitrificans
Read TextPreview

Extract of sample "Biology BSc third year biotech project; Manipulating Biomass and PHA Production in batch and continuous cultures of Paracoccus denitrificans"

Download file to see previous pages This proves that the product formation is dependent on the substrate concentration.
The concentration of the protein present in the given culture was determined using the Bradford assay at an absorbance of 595 nm. These readings are plotted against the standard protein data curve to find the actual concentration of the protein formed at various time intervals. It was found that the concentration of the protein was maximum after 69 hours.
The biomass concentration was found after the 96 hours of culture for the different concentrations of the substrate succinate. It is found that the biomass concentration was maximum for the 50mM concentration. As the PHB are found inside the bacteria, if the biomass is higher then the PHB concentration is also found to be higher. Thus indicating that at a succinate substrate concentration of 50mM, the biomass and the PHB concentration was found to be higher.
The biomass concentration was determined after the 96 hours culture. The concentration of the acetate varied from 10mM to 50mM. It is found that as the concentration of the acetate was increased, the biomass concentration was also found to increase. If the biomass concentration is higher then the PHB concentration must be also very high.
From the graph, it was found that the biomass concentration was maximum after 60 hours and the concentration of the protein was maximum during the third day of the culture. From this graph we can find that the concentration of the protein was maximum after the cells have crossed the log phase.
When the growth curve of P.denitrificans was observed in both continuous and anaerobic culture, it was found that the growth was higher in continuous culture. Comparatively the growth was 10 fold higher in the continuous culture
When the growth of the bacteria was compared in different concentrations of succinate at aerobic and anaerobic cultures, it was observed that the ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“Biology BSc third year biotech project; Manipulating Biomass and PHA Lab Report”, n.d.)
Retrieved from
(Biology BSc Third Year Biotech Project; Manipulating Biomass and PHA Lab Report)
“Biology BSc Third Year Biotech Project; Manipulating Biomass and PHA Lab Report”, n.d.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document

CHECK THESE SAMPLES OF Biology BSc third year biotech project; Manipulating Biomass and PHA Production in batch and continuous cultures of Paracoccus denitrificans

Biomass Energy

...that every year, one can spend 33% less on heating his/her home, which amounts to a substantial saving in a period of 10 or 15 years. Moreover, biomass is readily accessible in large quantities all over the globe – there is overabundance of agricultural and organic waste generated daily and biomass energy is generated from these wastes, thereby making large pipelines across oceans and country borders unnecessary (Ghosh, 4 &, 5). Disadvantages of Biomass Energy The extraction of biomasses can be quite expensive. In some regions, biomass projects may not be worth the price and...
3 Pages(750 words)Essay

Biomass Questions

...Biomass Questions BIOMASS QUESTIONS Titus Rock Manickam Order No. 326496 13 October 2009 BIOMASS QUESTIONS Lecture 1 - Answer 1 Disposal of garbage wastes is a challenge to any municipal corporation in the world. There is the possibility to use these wastes for energy production. Food wastes and green wastes emit methane gas on decomposition and methane gas can be utilized for energy production. Municipal Solid Wastes (MSW) have the potential for energy production but the capital cost for such projects may be very high and the revenues from energy generation may be quite low, thus rendering such...
3 Pages(750 words)Essay

Biology project

...Here Biology Project What is Cancer and its Causes How does cancer begin and spreads Chose a specific cancer. United States National Cancer Institute (1: par 1-2) defines cancer as a term used to describe diseases by which cells divide uncontrollably and are able to reach and invade other tissues. According to the institute, several studies discovered that there are more than 100 distinct types of cancer and mostly are identified based on the organ or type of cell that they start; it can migrate to other body parts via the blood and lymph systems. Obviously, The body is composed of several cell types; when these cells become old, worn or damaged, they naturally die and new cells are formed to replace the...
3 Pages(750 words)Essay

Final year project

...7.0 Bibliography 0 Qatar Guide: Introduction, Employment prospects Qatar allows plenty... 2.0 News Week, Qatar at the cross roads of change, 3.0 OBG, Oxford Business School, Qatar: Infrastructure Investments, 4.0 Investment Promotion Department, 5.0 Qatar law, Legal System in Qatar, 6.0 Doing Business in Qatar, 7.0 AME Info fn, Qatar Focus, Qatar's $65bn non-energy mega projects start to emerge, Net Group...
22 Pages(5500 words)Essay

Manipulating Statistics

...benefits. There is a sampling bias in the statement above because it does not consider unemployment under the categories of sickness, government trainees, and discouraged workers. Because the sample was biased, the result and analysis of unemployment may be incorrect. Another way of manipulating statistics is through spurious correlation. This usually happens when two variables have no direct and causal linked to each other, but both are linked to a third unreported variable. In the article mentioned above, this unauthentic correlation can be observed through the misuse of vacancy data. The job vacancy data may have no direct link with unemployment rate because other variables affect job vacancy...
1 Pages(250 words)Essay

BSc Dentistry Oral Biology

...developed for 192 children with congenitally missing teeth included clinical and radiographic tests. Exclusion criteria were presented by the anamnestic data about the possibility of teeth loosing due to trauma or extraction. Missing teeth were assessed accordingly to their types, localisations in dental arches. Results. After conducting appropriate statistical analysis the following important findings of the research were reported: 1. There are no statistically significant differences in the prevalence of hypodontia dependently on gender or localisation of lesion. Nevertheless bilateral hypodontia (73.2%) was more common than unilateral hypodontia (see Fig. 1). 2. The third molar was most frequently affected (38,4%)...
2 Pages(500 words)Essay

Mini-Project (Third)

...and contracts, and this at time when a cash inflow was most needed. Changes in project planning led to costs becoming uncontrollable and some of these were major. For example, software for the production train had to be rewritten and the building could not house the production train. The lack of schedule planning not only wasted time and caused delays but also resulted in the loss of income. For example, the several weeks that were lost in production bore little income but an excessive rise in costs. Also, “the costs of delays in completion were charged to Woody’s account.” Stocks of finished goods inventories were not monitored and allowed to be depleted. The expensive...
4 Pages(1000 words)Essay

Manipulating Data

...Manipulating Data Introduction A programming paradigm is a form of computer programming in which the elements and structureof computer programs are built. The programming languages are determined by the programming paradigms that they support. In the past 60 years, there have been many different ways through which programming has evolved in collective grouping and manipulating data by spaghetti code, structured programming, modular programming, and object-oriented programming. Spaghetti code is a particular programming code which was initially used in programming. It uses branching from one code to another. It is the result of an old code that is modified over the years...
2 Pages(500 words)Essay

Portfolio for Continuous Professional Development (CPD) for third year radiography students

...PORTFOLIO FOR CONTINUOUS PROFESSIONAL DEVELOPMENT (CPD) FOR THIRD YEAR RADIOGRAPHY As a in Radiography, I am eager to start my professional career. Radiography and imaging has a stress on building an information base of the ideas and standards of radiography, and restorative phrasing, to create key thoughts, and recognizing sources. The course additionally supports me in advancing exactness, discerning contention and presenting models of inquiry and the proof base that underpins radiography. The course has provided for me the parts for consideration and fundamental nursing abilities, creating radiographies aptitudes and creating capacity to consider hone (Eraut 2000). Furthermore, I have been furnished with aptitudes of creating... FOR...
4 Pages(1000 words)Essay

Windows Batch

...Windows Batch Background information In windows, a batch file is a script file that contains a series of commands that are interpreted with the use of the command line interpreter. It may contain any command that the interpreter accepts at the command prompt and they get interpreted one after the other. Windows batch is applicable in all the Windows versions such as XP, Vista and Windows 7. Importance It is a powerful tool for carrying out repetitive tasks, if you are creating volumes of files, you do not need much workforce to realize your goals. Network and system administration is much easier for both individuals and organizations applying Windows batch in their...
2 Pages(500 words)Assignment
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Lab Report on topic Biology BSc third year biotech project; Manipulating Biomass and PHA Production in batch and continuous cultures of Paracoccus denitrificans for FREE!

Contact Us