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Course work - Admission/Application Essay Example

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Immunohistochemistry (IHC) is a process in which the proteins in the biological tissue sections are localized by using the characteristic of the antibodies that they can bind specifically to the antigens. IHC staining is used to locate the abnormal cells, such as the cells of…
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Download file to see previous pages This fever is also referred to as infectious mononucleosis. Symptomatically it may cause two rare cancers Burkitt’s and Lymphoma nasopharangeal Carcinoma. If a person becomes infected with Epstein Barr virus in the life, antibodies against this virus develop in a great number and the reinfection can not make that person sick again. (S David & Tiansheng pp519–527)
Laboratory diagnosis is carried on a single acute-phase serum sample by testing for antibodies to many Epstein Barr virus associated antigens simultaneously. These laboratory tests can detect the presence as well as the concentration of specific EBV antibodies. By observing the result of these different tests the stage of EBV infection can be identified. The antigens immunohistochemically be detected in tissues by two methods. The direct method is a one step staining method. In this method only one labeled anti body such as FITC conjugated anti serum is used that directly reacts with the antigen in the tissue sections of the tonsils. The indirect method involves an unlabeled primary antibody and a labeled secondary antibody which had already been produced against the IGg of animals. In this method, the labeled secondary antibody reacts with the unlabeled primary antibody. Usually a biotinylated secondary antibody coupled with streptaviden-horseradish peroxidase is reacted with 3-D Diaminobenzidine (PAV) to produce a brown staining. The primary and secondary antibodies attach with each other. This process is known as DAB staining. The indirect method is more commonly used as compared to the direct one.
For tissue culture preparation, the tissue is kept on ice in phosphate-buffered saline and is processed for at least two to six hours after their removal from the patient. The cell pellets are fixed in paraffin wax in sectioned form. The sections are then dewaxed and are washed with ...Download file to see next pagesRead More
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