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Biotransport - Research Paper Example

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Oxygen is one of the important nutrients. The bypass to inefficient glycolytic metabolism is because of cellular hypoxia that is not direct resulting in a reduction of…
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Download file to see previous pages The model foresaw deadly values of pH (less than 6.5) in EHTs consisted of 106 cells per square centimeter. Predictions of pH slightly relied on oxygen concentration and strongly on carbon dioxide concentration and the length of diffusion path. In study conclusion, acidosis induced by hypoxia are an important factor in the mass transport problem.
The limitations of Transport of critical nutrients obstruct cardiac tissue engineering. The available EHT models comprise of high-density neonatal cardiomyocytes in biopolymer hydrogel or scaffold of synthetic porous polymer. After cultivation by a bioreactor, the cardiac tissue constructs make tiny synchronous contractions like that of differentiated cardiac myofibrils. The problem that prevailed was of scaling the tissue constructs to clinical size and dead cells inside the construct. Reports emphasize on the importance of oxygen as a metabolite in cardiac tissue engineering and engineered cartilage. Reports has it that oxygen concentrations of less than 14 micro molar exist in 1mm EHT model solution and the rate of consumption of oxygen by cardiomyocyte finishes it to above zero.
Laboratory data suggest that reduced oxygen does not cause cell death due to hypoxia. Acidosis initiate apoptosis. Lactate-induced acidosis of culture medium at pH 6 stimulates apoptosis in cultured cardiomyocytes. There is expectation that pH and oxygen have functions to play in tissue engineering mass transport problem.
Generally, the work done was to analyze the pH gradients resulting from mass transport limitations in engineered heart tissue (EHT). Brown obtained neonatal rat ventricular myocytes (NRVM) for experiment. He noticed variations in the hypoxia response in acidification of culture medium in relation to the source and procurement method. Development of EHTs constructs succeeded Eschenhagen lab method plus some modifications. He neutralized 3.2mg/mL ...Download file to see next pagesRead More
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