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Analytical Method - Essay Example

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The major purpose of the article is to develop a quick and efficient method based on liquid chromatography–quadrupole time-offlight mass spectrometry (LC–QTOF) in order to detect and identify elemental profile of gunshot residues (GSR). In which case, the…
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Analytical Method
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Download file to see previous pages This formed the problem statement for the article thereby triggering the researchers to seek for the most appropriate tools and techniques for achieving their objectives. In which case, the researchers provide the theoretical background asserting that the chemical analysis of GSR can either focus on inorganic or organic components of the analyte therein. Intuitively, flame ionization detector (FID), thermal energy analysis (TEA), electron capture detector (ECD) and mass spectrometry (MS) together with Gas chromatography are important aspects which could be considered in this OGSR analysis.
For the methods and materials, the use of MS/MS mode guided in explicit identification of target analyte. The researchers majorly focused on liquid chromatography tandem mass spectrometry (LC–MS/MS) coupled with GC; as evident in the use of LC–MS grade methanol used in the mobile phase and in Chromatographic analysis was performed on an Agilent 1200 Series HPLC system coupled with a hybrid quadrupole time-of-flight mass spectrometer. The QTOF system was core to facilitating analysis in MS or MS/MS mode. The researchers carried out accurate mass measurement of the precursor ion, within MS spectra, mass measurement of the most specific product ion, and the retention time of the analyte compared with the retention time of the standard. Swabs were compared with home-modified tape lift supports covered with a PTFE layer to determine the better sampling technique. The modified tape lift provided better extraction recoveries and enabled the analysis of inorganic and organic GSR simultaneously.
In Optimization of LC–QTOF in MS/MS mode parameters, different columns were tested to ensure optimization of the composition of the mobile phase through unequivocally separating and ionizing the analytes. In which case, chances for getting false positive and false negative results ...Download file to see next pagesRead More
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