quora
Nobody downloaded yet

Southern blotting application - Assignment Example

Comments (0) Cite this document
Summary
Restriction Fragment Length Polymorphism (RFLP) is a technique that involves the detection of differences in homologous DNA sequences by the presence of different lengths of fragments after digestion of the DNA samples in question with specific restriction endonucleases. RFLP is…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER93.6% of users find it useful
Southern blotting application
Read TextPreview

Extract of sample
"Southern blotting application"

Download file to see previous pages A labeled DNA sequence is usually an RFLP probe that hybridizes with either a single or multiple fragments of the digested DNA sample after separation by gel electrophoresis resulting to showing a unique blotting pattern attributed to a genotype that is specific to a locus. RFLP probes are usually short, single or low copy genomic DNA or cDNA clones. Application of RFLP probes are usually used in variation analysis such as in paternity tests, ping and diagnosis of hereditary diseases. RFLP probes are also used in genome mapping.
RFLPs are detected by using restriction enzyme that can recognize and fragment a sample of DNA where there is a specific short sequence. This step is known as restriction enzyme digestion. Single nucleotide polymorphisms can either create or destroy restriction endonuclease recognition sites therefore affecting the length and the quantity of DNA fragments resulting from restriction enzyme digestion.
Agarose gel electrophoresis separates the resulting DNA fragments and transferred to a membrane through a Southern blot procedure. The membrane is a paper like matrix made up of nitrocellulose and usually has a slight positive charge. The fragment lengths that are complementary to the RFLP probes and are considered as alleles in genetic analysis is determined by the hybridization of the membrane to the labeled DNA probe (National Center for Biotechnology Information ).
In developing RFLP probes, a methylation sensitive enzyme digests the total DNA thus enriching the library for sequences that are single or low copy expressed. The digested DNA is size fractionated on preparative agarose gel and excision, elusion and cloning into plasmid vector of is done. The digested plasmids are consequently screened for inserts. Total sheared DNA can be used to probe southern blots of the inserts to select clones that can hybridise to single and low copy sequences. Genomic DNA of different genotypes ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Southern blotting application Assignment Example | Topics and Well Written Essays - 750 words”, n.d.)
Retrieved from https://studentshare.org/biology/1680041-southern-blotting-application
(Southern Blotting Application Assignment Example | Topics and Well Written Essays - 750 Words)
https://studentshare.org/biology/1680041-southern-blotting-application.
“Southern Blotting Application Assignment Example | Topics and Well Written Essays - 750 Words”, n.d. https://studentshare.org/biology/1680041-southern-blotting-application.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Application Assignment
In the text, the main variable throughout the study focus on the perceived non verbal responses of students in a classroom setting. On the other hand, the research examines on the variables of teacher’s level of communication apprehension, nonverbal immediacy and nonverbal confirmation.
5 Pages(1250 words)Assignment
Southern Culture Class What direction is the South heading in Politically
However, the territory is drifting towards been a blue state due to influence of immigrants. The conservative aspect associated with southern politics has its root in the era of slave trade. Since much of southern states wealth was inform of slaves,
1 Pages(250 words)Assignment
Application 6.2
The key programs targeted by the institution are the executive MBA (EMBA), Masters in Organizational Development (MSOD) and key / presidential Executive MBA (PKE). With specific reference to a cost-cutting initiative to be adopted at
2 Pages(500 words)Assignment
Application 5.1
The Steinway brand is highly priced than the Boston brand. Using New York and Germany as the main centers of operation for handcrafting the grand pianos, the company uses a cumulative amount of more than 200 dealers who are independent
2 Pages(500 words)Assignment
IS-LM model Application
In this case, the influence of Chief Executive Officer of RBC is surrounded and also swayed by the reputation the editors want the organization to perceive. Nevertheless, the response is very concrete when addressing moral materials and situations
1 Pages(250 words)Assignment
Mobile application
will include popular games and other mobile applications such as Apple iOS 4.1, Sky + Remote Record Android, Facebook for Android 1.3, Skype for iOS4, etc. Only the application that is supported by common devices such as, Windows Mobile, Blackberry, Java, etc, will be added to
1 Pages(250 words)Assignment
Application Case 3-1
d incidents of sexual harassment; but the case was silent with regards to how many of the employees actually filed a legal complaint against the president. If the company’s progressive discipline policies indicate that after a determined number of reportedly filed and verified
1 Pages(250 words)Assignment
Application Assignment
The training program was prepared for a group of eight unit managers for the Nursing Division who would be in charge of governing the operations of different nursing units. These managers do not have any health care or nursing background but are graduates of masteral
3 Pages(750 words)Assignment
Job Application
Moreover in my possession are the key strengths for this position, and they include: Currently, I am studying a Bachelor’s degree in Business
2 Pages(500 words)Assignment
Business Application
s.1 The focus of this assignment is to examine the application of ERP in mining and manufacturing companies taking consideration of the advantages, disadvantages and limitations posed by the ERP in those companies.2 The analysis will be based in the case study of Oracle
8 Pages(2000 words)Assignment
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Assignment on topic Southern blotting application for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us