Extract of sample "ISOLATION AND CHARACTERISATION OF LYMPHOCYTES"
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This is because this results in an inaccurate count. The same is true if the cover slip is moved after the sample is loaded. To make randomized counting only the 4 squares at each end were picked and then the 16 small squares within each large square, that is, in an organized zigzag along the rows. To make the counting standardized only cells lying on the bottom and right hand boundary were counted in and a cell lying on the top or left hand boundary were not counted in. This was in order to avoid counting cells twice. Normally, at least two squares should be counted, comprising more than 100 cells within each central counting area of every square. For higher accuracy, additional cells can be counted and the mean used to compute cell concentration. The result of cells counts were in the table 1 below.
The other cells in the splenocyte preparation are red cells 3.82%, Granulocytes 6.1% as shown in the table 4 above. The abundance of small lymphocytes shows that the cell suspension has more immature cells which are maturing in the spleen to become large lymphocytes and T-lymphocytes. With time the population of small lymphocytes decreases as they develop to large lymphocytes, medium lymphocytes and T-lymphocytes. The spleen is made up of immune lymphocytes and that why in this experiment there were low number of other types of cells. Time was very important in this lab because long exposure could lead to lack of the reliability. This is because the population of splenocytes keep on changing since some cell has not matured fully and other are multiplying. For this lab the time limit was hour since
In this lab exercise, Nigrosin was used to stain any dead cells. Nigrosin is an acidic stain. This means the stain readily gives up a hydrogen ion and becomes negatively charged. Since the surface of dead cells is negatively charged, the cell surface repels the stain. Thus slide glass will stain, but the dead cell will not. The dead
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The polyphenols in tea are known to have antioxidant properties which can quench free radical species, and also pro-oxidant activities that appear to be responsible for the induction of apoptosis in tumor cells. The bioavailability of these natural compounds is an important factor that determines their efficacy.
Apoptosis is a morphologically distinct form of cell death which is under genetic control. It is also well recognized that apoptosis and the genes that control it have a strong effect on the malignant phenotype. The genes found mutated most frequently in colon cancer are adenomatous polyposis coli (APC), deleted in colon cancer (DCC), K-Ras, and p53 (Bhatnagar et al., 2009).
The author analyzes the importance of accurate identification of bacteria and the necessary materials and methods. He shows newer procedures of rapid identification of bacteria like mass spectrometry and computional analysis, microarray-based identification and other that allow faster and affective method of identifying bacteria and other microorganisms.
al. 2007], melanomas [Fang et. al. 2005], prostate [Patrawala et. al. 2006], and pancreas [Gou et. al. 2007]. This characteristic property of purified CSCs is being exploited for their enrichment from tumor cultures. The culturing of cells from
In our experiment we will have to identify the presence of bacteria that may cause a food born disease in lettuce. Lettuce can often be contaminated with different bacteria that can cause food born disease. Lettuce can be contaminated with dirt, fecal
In addition, the inclusion of these values was rather important to help in the calculation of the total and specific activities for AChE in the result as well as the total \protein content in the results.
Ceramic Raschig ring has high surface area and moderate surface area as a result of its structure that is cylindrical hollow, therefore it is applicable as microorganism’s solid support, in which greater rate of mass transfer is attainable. In this report, the characteristics of hydrodynamic which include: material density, bulk density, etc.