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The main objective of this laboratory exercise was to examine the principles of isolation and quantitation of antigens. In particular, the study aimed at harvesting and…
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Download file to see previous pages This is because this results in an inaccurate count. The same is true if the cover slip is moved after the sample is loaded. To make randomized counting only the 4 squares at each end were picked and then the 16 small squares within each large square, that is, in an organized zigzag along the rows. To make the counting standardized only cells lying on the bottom and right hand boundary were counted in and a cell lying on the top or left hand boundary were not counted in. This was in order to avoid counting cells twice. Normally, at least two squares should be counted, comprising more than 100 cells within each central counting area of every square. For higher accuracy, additional cells can be counted and the mean used to compute cell concentration. The result of cells counts were in the table 1 below.
The other cells in the splenocyte preparation are red cells 3.82%, Granulocytes 6.1% as shown in the table 4 above. The abundance of small lymphocytes shows that the cell suspension has more immature cells which are maturing in the spleen to become large lymphocytes and T-lymphocytes. With time the population of small lymphocytes decreases as they develop to large lymphocytes, medium lymphocytes and T-lymphocytes. The spleen is made up of immune lymphocytes and that why in this experiment there were low number of other types of cells. Time was very important in this lab because long exposure could lead to lack of the reliability. This is because the population of splenocytes keep on changing since some cell has not matured fully and other are multiplying. For this lab the time limit was hour since
In this lab exercise, Nigrosin was used to stain any dead cells. Nigrosin is an acidic stain. This means the stain readily gives up a hydrogen ion and becomes negatively charged. Since the surface of dead cells is negatively charged, the cell surface repels the stain. Thus slide glass will stain, but the dead cell will not. The dead ...Download file to see next pagesRead More
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